Holographic interferometry as a method to study conformational changes in macromolecules
This paper concerns with our measurements of reaction center (RC) volume changes in the course of photoactivation using the method of holographic interferometry. As a principal value, we studied changes of a solution refraction index (Δn) influenced by the sample volume expansion due to temperature...
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| Дата: | 2002 |
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| Автори: | , , , |
| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
Інститут фізики напівпровідників імені В.Є. Лашкарьова НАН України
2002
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| Назва видання: | Semiconductor Physics Quantum Electronics & Optoelectronics |
| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/121293 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Holographic interferometry as a method to study conformational changes in macromolecules / Y. Barabash, V. Kharkyanen, M. Zabolotny, N. Sokolov // Semiconductor Physics Quantum Electronics & Optoelectronics. — 2002. — Т. 5, № 3. — С. 307-311. — Бібліогр.: 5 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| id |
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irk-123456789-1212932017-06-14T13:40:32Z Holographic interferometry as a method to study conformational changes in macromolecules Barabash, Y. Kharkyanen, V. Zabolotny, M. Sokolov, N. This paper concerns with our measurements of reaction center (RC) volume changes in the course of photoactivation using the method of holographic interferometry. As a principal value, we studied changes of a solution refraction index (Δn) influenced by the sample volume expansion due to temperature changes (ΔnT), light absorption (Δnabs) and changes in the volume of photoactive molecules (DnV). Using the scheme of temperature compensation we could reduce the task to the case ΔnT <<Δnabs, ΔnV. At RC concentrations close to 10¹⁵ cm-³, our experimental setup enabled to register the shift of an interference pattern up to 0.1 of the bandwidth. Our estimations for the case of a protein solution yields in relative changes in the molecular volume within 10-³...10-². 2002 Article Holographic interferometry as a method to study conformational changes in macromolecules / Y. Barabash, V. Kharkyanen, M. Zabolotny, N. Sokolov // Semiconductor Physics Quantum Electronics & Optoelectronics. — 2002. — Т. 5, № 3. — С. 307-311. — Бібліогр.: 5 назв. — англ. 1560-8034 PACS: 42.40.K http://dspace.nbuv.gov.ua/handle/123456789/121293 en Semiconductor Physics Quantum Electronics & Optoelectronics Інститут фізики напівпровідників імені В.Є. Лашкарьова НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| description |
This paper concerns with our measurements of reaction center (RC) volume changes in the course of photoactivation using the method of holographic interferometry. As a principal value, we studied changes of a solution refraction index (Δn) influenced by the sample volume expansion due to temperature changes (ΔnT), light absorption (Δnabs) and changes in the volume of photoactive molecules (DnV). Using the scheme of temperature compensation we could reduce the task to the case ΔnT <<Δnabs, ΔnV. At RC concentrations close to 10¹⁵ cm-³, our experimental setup enabled to register the shift of an interference pattern up to 0.1 of the bandwidth. Our estimations for the case of a protein solution yields in relative changes in the molecular volume within 10-³...10-². |
| format |
Article |
| author |
Barabash, Y. Kharkyanen, V. Zabolotny, M. Sokolov, N. |
| spellingShingle |
Barabash, Y. Kharkyanen, V. Zabolotny, M. Sokolov, N. Holographic interferometry as a method to study conformational changes in macromolecules Semiconductor Physics Quantum Electronics & Optoelectronics |
| author_facet |
Barabash, Y. Kharkyanen, V. Zabolotny, M. Sokolov, N. |
| author_sort |
Barabash, Y. |
| title |
Holographic interferometry as a method to study conformational changes in macromolecules |
| title_short |
Holographic interferometry as a method to study conformational changes in macromolecules |
| title_full |
Holographic interferometry as a method to study conformational changes in macromolecules |
| title_fullStr |
Holographic interferometry as a method to study conformational changes in macromolecules |
| title_full_unstemmed |
Holographic interferometry as a method to study conformational changes in macromolecules |
| title_sort |
holographic interferometry as a method to study conformational changes in macromolecules |
| publisher |
Інститут фізики напівпровідників імені В.Є. Лашкарьова НАН України |
| publishDate |
2002 |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/121293 |
| citation_txt |
Holographic interferometry as a method to study conformational changes in macromolecules / Y. Barabash, V. Kharkyanen, M. Zabolotny, N. Sokolov // Semiconductor Physics Quantum Electronics & Optoelectronics. — 2002. — Т. 5, № 3. — С. 307-311. — Бібліогр.: 5 назв. — англ. |
| series |
Semiconductor Physics Quantum Electronics & Optoelectronics |
| work_keys_str_mv |
AT barabashy holographicinterferometryasamethodtostudyconformationalchangesinmacromolecules AT kharkyanenv holographicinterferometryasamethodtostudyconformationalchangesinmacromolecules AT zabolotnym holographicinterferometryasamethodtostudyconformationalchangesinmacromolecules AT sokolovn holographicinterferometryasamethodtostudyconformationalchangesinmacromolecules |
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2023-10-18T20:39:06Z |
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2023-10-18T20:39:06Z |
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