Maltose­binding protein switches programmed cell death in Nicotiana glutinosa leaf cells

• Видавець: Інститут клітинної біології та генетичної інженерії НАН України
• Дата: 2014
• Автор: Gholizadeh, A.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут клітинної біології та генетичної інженерії НАН України 2014
• Назва видання: Цитология и генетика
• Теми: Оригинальные работы
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/126622
• Цитувати: Maltose­binding protein switches programmed cell death in Nicotiana glutinosa leaf cells / A. Gholizadeh // Цитология и генетика. — 2014. — Т. 48, № 2. — С. 20-27. — Бібліогр.: 36 назв. — англ.

Maltose-binding protein (MBP) is a part of the complex regulatory and transport maltose system of Escherichia coli that is responsible for the uptake and efficient catabolism of maltodextrins through the trans-membrane signaling at the expense of ATP. In the present work, this bacterial periplasmic protein was identified as a cell death inducer in Nicotiana glutinosa plant. Upon exogenous application at the concentrations more than 50 μg/mL, purified MBP protein induced wilting and localized cell death on the leaves of test plant. DNA fragmentation assay and antioxidant enzymes activity test showed that the induced cell death might be programmed. It was predicted that maltose-binding protein signals programmed cell death (PCD) upstream of reactive oxygen species (ROS) and DNA fragmentation processes in the test plant leaves. However, it needs to be clarified that how MBP switches and signals PCD in plant tissues.