P element temperature-specific transposition: a model for possible regulation of mobile elements activity by pre-mRNA secondary structure

• Дата: 2014
• Автори: Gultyaev, A., Redchuk, T., Korolova, A., Kozeretska, I.P
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут клітинної біології та генетичної інженерії НАН України 2014
• Назва видання: Цитология и генетика
• Теми: Оригинальные работы
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/126673
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: P element temperature-specific transposition: a model for possible regulation of mobile elements activity by pre-mRNA secondary structure / A. Gultyaev, T. Redchuk, A. Korolova, I.P Kozeretska // Цитология и генетика. — 2014. — Т. 48, № 6. — С. 40-44. — Бібліогр.: 30 назв. — англ.

P element is a DNA transposon, known to spread in genome using transposase activity. Its activity is tissue-specific and normally observed at high temperatures within 24°C to 29°C. Here, we present a predicted RNA secondary structure domain of P element pre-mRNA which could potentially regulate the temperature sensitivity of the P element activity. In canonical P elements, the structure is a small hairpin with double-helical part interrupted by a symmetric loop and a mismatch. In M type P elements, the A.A mismatch is substituted by an A-U base pair, stabilizing the structure. The hairpin structure covers the region involving the IVS-3 5′ splice site and both pseudo-splice sites F1 and F2. While the IVS-3 and F1 binding sites of U1 snRNA are located in the double-stranded part of the structure, the F2 site is exposed in the hairpin loop. The formation of this structure may interfere with landing of U1 snRNA on IVS-3 site, while F2 is positioned for the interaction. Alignment of P element sequences supports the proposed existence of the hairpin, showing high similarity for this region. The hairpin structure, stable at low temperatures, may prevent correct IVS-3 splicing. Conversely, temperature-induced destabilization of the hairpin structure may result in the splicing at the proper IVS-3 splice site. Taking into account the increasing amount of data demonstrating the important influence of RNA folding on phenotypes determined by alternative splicing a model for possible regulation of the activity of mobile elements by pre-mRNA secondary structure seems intriguing.