The possible involvement of D-amino acids or their metabolites in Arabidopsis cysteine proteinase/cystatin-dependent proteolytic pathway
Cysteine proteinases and their inhibitors ‘cystatins’ play essential roles in plant growth and development. They are involved in various signaling pathways and in the response to wide ranges of biotic and abiotic environmental stresses. To investigate their possible influence from D-amino acids or t...
Збережено в:
| Дата: | 2015 |
|---|---|
| Автор: | |
| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
Інститут клітинної біології та генетичної інженерії НАН України
2015
|
| Назва видання: | Цитология и генетика |
| Теми: | |
| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/126707 |
| Теги: |
Додати тег
Немає тегів, Будьте першим, хто поставить тег для цього запису!
|
| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | The possible involvement of D-amino acids or their metabolites in Arabidopsis cysteine proteinase/cystatin-dependent proteolytic pathway / A. Gholizadeh // Цитология и генетика. — 2015. — Т. 49, № 2. — С. 3-10. — Бібліогр.: 30 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| id |
irk-123456789-126707 |
|---|---|
| record_format |
dspace |
| spelling |
irk-123456789-1267072017-12-02T03:03:25Z The possible involvement of D-amino acids or their metabolites in Arabidopsis cysteine proteinase/cystatin-dependent proteolytic pathway Gholizadeh, A. Оригинальные работы Cysteine proteinases and their inhibitors ‘cystatins’ play essential roles in plant growth and development. They are involved in various signaling pathways and in the response to wide ranges of biotic and abiotic environmental stresses. To investigate their possible influence from D-amino acids or their metabolism in vivo, Arabidopsis seedlings were allowed to grow under four physico-chemically different D-amino acids including D-aspartate, D-serine, D-alanine and D-phenylalanine containing media. The reverse transcription polymerase chain reaction (RT-PCR) analysis of cysteine proteinase and cystatin gene expressions showed that the addition of D-amino acid to the plant growth media considerably induce the expression of proteinase transcript while decrease the expression level of inhibitor gene in the leaf and root tissues of the test plant in overall. Based on the obtained results the potential impact of D-amino acids or their metabolism on the activity of cysteine proteinase/cystatin-dependent proteolytic apparatus as well as their possible cooperation were predicted and discussed in the plant system. Цистеинпротеиназы и их ингибиторы цистатины играют существенную роль в росте и развитии ратений. Они вовлечены в различные сигнальные пути и в ответ на широкий спектр биотических и абиотических стрессов. Для изучения возможного влияния на них D-аминокислот или их метаболизма in vivo проростки Arabidopsis выращивали на средах в присутствии четырех физико-химически различных аминокислот, включая D-аспартат, D-серин, D-аланин и D-фенилаланин. RT-PCR анализ цистеин-протеиназы и экспрессии гена цистатина показал, что добавление D-аминокислот в среду для выращивания растений значительно индуцировало экспрессию транскриптов протеиназ, в то же время снижая уровень экспрессии гена-ингибитора в тканях листьев и корней исследуемых растений. На основании полученных результатов обсуждается потенциальное влияние D-аминокислот или их метаболизма на активность в цистеинпротеиназа/цистатин-зависимом протеолитическом пути, а также ихвозможное взаимодействие в растительной системе. The present work was financially supported by Research Institute for Fundamental Sciences (RIFS), University of Tabriz, Iran. The authors of this paper are thanks for their supporting. 2015 Article The possible involvement of D-amino acids or their metabolites in Arabidopsis cysteine proteinase/cystatin-dependent proteolytic pathway / A. Gholizadeh // Цитология и генетика. — 2015. — Т. 49, № 2. — С. 3-10. — Бібліогр.: 30 назв. — англ. 0564-3783 DOI: 10.3103/S0095452715020036 http://dspace.nbuv.gov.ua/handle/123456789/126707 en Цитология и генетика Інститут клітинної біології та генетичної інженерії НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Оригинальные работы Оригинальные работы |
| spellingShingle |
Оригинальные работы Оригинальные работы Gholizadeh, A. The possible involvement of D-amino acids or their metabolites in Arabidopsis cysteine proteinase/cystatin-dependent proteolytic pathway Цитология и генетика |
| description |
Cysteine proteinases and their inhibitors ‘cystatins’ play essential roles in plant growth and development. They are involved in various signaling pathways and in the response to wide ranges of biotic and abiotic environmental stresses. To investigate their possible influence from D-amino acids or their metabolism in vivo, Arabidopsis seedlings were allowed to grow under four physico-chemically different D-amino acids including D-aspartate, D-serine, D-alanine and D-phenylalanine containing media. The reverse transcription polymerase chain reaction (RT-PCR) analysis of cysteine proteinase and cystatin gene expressions showed that the addition of D-amino acid to the plant growth media considerably induce the expression of proteinase transcript while decrease the expression level of inhibitor gene in the leaf and root tissues of the test plant in overall. Based on the obtained results the potential impact of D-amino acids or their metabolism on the activity of cysteine proteinase/cystatin-dependent proteolytic apparatus as well as their possible cooperation were predicted and discussed in the plant system. |
| format |
Article |
| author |
Gholizadeh, A. |
| author_facet |
Gholizadeh, A. |
| author_sort |
Gholizadeh, A. |
| title |
The possible involvement of D-amino acids or their metabolites in Arabidopsis cysteine proteinase/cystatin-dependent proteolytic pathway |
| title_short |
The possible involvement of D-amino acids or their metabolites in Arabidopsis cysteine proteinase/cystatin-dependent proteolytic pathway |
| title_full |
The possible involvement of D-amino acids or their metabolites in Arabidopsis cysteine proteinase/cystatin-dependent proteolytic pathway |
| title_fullStr |
The possible involvement of D-amino acids or their metabolites in Arabidopsis cysteine proteinase/cystatin-dependent proteolytic pathway |
| title_full_unstemmed |
The possible involvement of D-amino acids or their metabolites in Arabidopsis cysteine proteinase/cystatin-dependent proteolytic pathway |
| title_sort |
possible involvement of d-amino acids or their metabolites in arabidopsis cysteine proteinase/cystatin-dependent proteolytic pathway |
| publisher |
Інститут клітинної біології та генетичної інженерії НАН України |
| publishDate |
2015 |
| topic_facet |
Оригинальные работы |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/126707 |
| citation_txt |
The possible involvement of D-amino acids or their metabolites in Arabidopsis cysteine proteinase/cystatin-dependent proteolytic pathway / A. Gholizadeh // Цитология и генетика. — 2015. — Т. 49, № 2. — С. 3-10. — Бібліогр.: 30 назв. — англ. |
| series |
Цитология и генетика |
| work_keys_str_mv |
AT gholizadeha thepossibleinvolvementofdaminoacidsortheirmetabolitesinarabidopsiscysteineproteinasecystatindependentproteolyticpathway AT gholizadeha possibleinvolvementofdaminoacidsortheirmetabolitesinarabidopsiscysteineproteinasecystatindependentproteolyticpathway |
| first_indexed |
2023-10-18T20:51:26Z |
| last_indexed |
2023-10-18T20:51:26Z |
| _version_ |
1796151287909187584 |