Circadian rhythms of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma
Aim: To study circadian rhythms (CR) of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma were compared with those in healthy men. Methods: The NK-cell and phagocyte cytotoxic activity in five patients with malignant melanoma stage I or II and 12 healthy do...
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| Дата: | 2006 |
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| Автори: | , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2006
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/134156 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Circadian rhythms of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma / N.F. Gamaleia, L.M. Skivka, A.G. Fedorchuk, E.D. Shishko // Experimental Oncology. — 2006. — Т. 28, № 1. — С. 54-590. — Бібліогр.: 36 назв. — англ. |
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irk-123456789-1341562018-06-13T03:03:11Z Circadian rhythms of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma Gamaleia, N.F. Skivka, L.M. Fedorchuk, A.G. Shishko, E.D. Original contributions Aim: To study circadian rhythms (CR) of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma were compared with those in healthy men. Methods: The NK-cell and phagocyte cytotoxic activity in five patients with malignant melanoma stage I or II and 12 healthy donors has been assessed by radioimmune assay and NBT-test. Results: The circadian rhythmicity in NK-cells and phagocyte activity in all cancer patients under study has been disrupted. The extent of such disruption tended to increase in patients with more advanced cancer. The most typical alterations were discoordination between the cytotoxicity rhythms of NK-cells and phagocytes (synchronized in healthy persons) and alterations in basic rhythm parameters: phase shifts and amplitude damping. Conclusion: In melanoma patients the significant alteration of CR in NK-cells and phagocytes cytotoxic activity was revealed. In spite of individual variations, the degree of the rhythm disruption basically depended on a disease stage. The alteration of CR phase and amplitude and discoordination between the rhythms of NK- cells and phagocyte were registered in all cases studied. Цель: изучить циркадианные ритмы (ЦР) цитотоксической активности мононуклеарных клеток периферической кро- ви (МКПК) больных злокачественной меланомой по сравнению с таковыми здоровых доноров. Методы: активность НК-клеток и МКПК, выделенных из крови 5 больных со злокачественной меланомой I и II стадий и 12 здоровых доноров, оценивали при помощи радиоиммунного метода и NBT-теста. Результаты: установлено, что у онкологических больных нарушена нормальная циркадианная ритмичность активности НК-клеток и МКПК, причем степень выраженности таких нарушений возрастает параллельно с прогрессией заболевания. К наиболее типичным изменениям относятся дискоорди- нация между цитотоксическими ритмами НК-клеток и мононуклеарными фагоцитами (синхронизированными у здоровых доноров) и изменения основных параметров ритма: сдвиг фаз и смещения амплитуды. Выводы: у больных меланомой выяв- лены значительные изменения ЦР НК-клеток и МКПК, причем степень нарушения ритма зависит от стадии заболевания. Во всех изученных случаях выявлены изменения фаз и амплитуды ЦР, декоординация ритмов НК-клеток и МКПК. Ключевые слова: злокачественная меланома, циркадианный ритм, активность НК-клеток, цитотоксическая активность мононуклеарных клеток. 2006 Article Circadian rhythms of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma / N.F. Gamaleia, L.M. Skivka, A.G. Fedorchuk, E.D. Shishko // Experimental Oncology. — 2006. — Т. 28, № 1. — С. 54-590. — Бібліогр.: 36 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/134156 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Gamaleia, N.F. Skivka, L.M. Fedorchuk, A.G. Shishko, E.D. Circadian rhythms of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma Experimental Oncology |
| description |
Aim: To study circadian rhythms (CR) of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma were compared with those in healthy men. Methods: The NK-cell and phagocyte cytotoxic activity in five patients with malignant melanoma stage I or II and 12 healthy donors has been assessed by radioimmune assay and NBT-test. Results: The circadian rhythmicity in NK-cells and phagocyte activity in all cancer patients under study has been disrupted. The extent of such disruption tended to increase in patients with more advanced cancer. The most typical alterations were discoordination between the cytotoxicity rhythms of NK-cells and phagocytes (synchronized in healthy persons) and alterations in basic rhythm parameters: phase shifts and amplitude damping. Conclusion: In melanoma patients the significant alteration of CR in NK-cells and phagocytes cytotoxic activity was revealed. In spite of individual variations, the degree of the rhythm disruption basically depended on a disease stage. The alteration of CR phase and amplitude and discoordination between the rhythms of NK- cells and phagocyte were registered in all cases studied. |
| format |
Article |
| author |
Gamaleia, N.F. Skivka, L.M. Fedorchuk, A.G. Shishko, E.D. |
| author_facet |
Gamaleia, N.F. Skivka, L.M. Fedorchuk, A.G. Shishko, E.D. |
| author_sort |
Gamaleia, N.F. |
| title |
Circadian rhythms of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma |
| title_short |
Circadian rhythms of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma |
| title_full |
Circadian rhythms of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma |
| title_fullStr |
Circadian rhythms of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma |
| title_full_unstemmed |
Circadian rhythms of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma |
| title_sort |
circadian rhythms of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2006 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/134156 |
| citation_txt |
Circadian rhythms of cytotoxic activity in peripheral blood mononuclear cells of patients with malignant melanoma / N.F. Gamaleia, L.M. Skivka, A.G. Fedorchuk, E.D. Shishko // Experimental Oncology. — 2006. — Т. 28, № 1. — С. 54-590. — Бібліогр.: 36 назв. — англ. |
| series |
Experimental Oncology |
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AT gamaleianf circadianrhythmsofcytotoxicactivityinperipheralbloodmononuclearcellsofpatientswithmalignantmelanoma AT skivkalm circadianrhythmsofcytotoxicactivityinperipheralbloodmononuclearcellsofpatientswithmalignantmelanoma AT fedorchukag circadianrhythmsofcytotoxicactivityinperipheralbloodmononuclearcellsofpatientswithmalignantmelanoma AT shishkoed circadianrhythmsofcytotoxicactivityinperipheralbloodmononuclearcellsofpatientswithmalignantmelanoma |
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2023-10-18T21:07:31Z |
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2023-10-18T21:07:31Z |
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