Expression profiling of cyclin B1 and D1 in cervical carcinoma
Aim: Cyclins are a family of regulatory proteins that play a key role in controlling the cell cycle. Abnormalities of cell cycle regulators, including cyclins and cyclin dependent kinases, have been reported in various malignant tumors. This study was undertaken to quantitatively detect cyclin B1 an...
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| Дата: | 2006 |
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| Автори: | , , , , , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2006
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/134157 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Expression profiling of cyclin B1 and D1 in cervical carcinoma / M. Zhao, Y.T. Kim, // Experimental Oncology. — 2006. — Т. 28, № 1. — С. 44-48. — Бібліогр.: 23 назв. — англ. |
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irk-123456789-1341572018-06-13T03:03:35Z Expression profiling of cyclin B1 and D1 in cervical carcinoma Zhao, M. Kim, Y.T. Yoon, B.S. Kim, S.W. Kang, M.H. Kim, S.H. Kim, J.H. Kim, J.W. Park, Y.W. Original contributions Aim: Cyclins are a family of regulatory proteins that play a key role in controlling the cell cycle. Abnormalities of cell cycle regulators, including cyclins and cyclin dependent kinases, have been reported in various malignant tumors. This study was undertaken to quantitatively detect cyclin B1 and D1 in cervical cancer. Methods: A quantitative real-time reverse transcription polymerase chain reaction and Western blot assay were used to analyze the expression of cyclin B1/D1 mRNA and proteins, respectively, in fresh invasive cervical cancer (n = 41) and normal cervical tissues (n = 10). Results: There was significantly greater cyclin B1 expression in invasive cervical cancer than in normal cervical tissue (P = 0.019). However, cyclin D1 expression was not significantly different. A Western blot assay yielded similar results. Conclusion: Our results were consistent with the concept that up-regulation of cyclin B1 expression occurred in cervical cancer and an aberrant expression of cyclin B1 might play an important role in cervical carcinogenesis. Цель: циклины представляют собой семейство регуляторных белков, контролирующих клеточный цикл. Наличие функциональных и структурных нарушений регуляторов клеточного цикла (циклинов и циклинзависимых киназ) было отмечено в клетках различных злокачественных новообразований. Целью данного исследования было проведение количественного определения циклинов B1 и D1 в клетках рака шейки матки. Методы: определение уровня экспрессии циклинов B1/D1 (mRNA и белков соответственно) в свежеполученных клетках инвазивного рака шейки матки (n = 41) и нормальной ткани шейки матки (n = 10) проводили методами RT-PCR в режиме реального времени и Вестерн-блот анализа. Результаты: отмечен более высокий уровень экспрессии гена циклина В1 в клетках инвазивного рака шейки матки, чем в клетках нормальной ткани (P = 0,019). Не выявлены значительные различия в уровне экспрессии гена циклина D1. При Вестерн-блот анализе получены аналогичные результаты. Выводы: результаты исследования подтверждают концепцию об активации экспрессии циклина В1 при раке шейки матки. Аберрантная экспрессия циклина В1 может играть важную роль при злокачественной трансформации эпителия шейки матки. 2006 Article Expression profiling of cyclin B1 and D1 in cervical carcinoma / M. Zhao, Y.T. Kim, // Experimental Oncology. — 2006. — Т. 28, № 1. — С. 44-48. — Бібліогр.: 23 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/134157 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Zhao, M. Kim, Y.T. Yoon, B.S. Kim, S.W. Kang, M.H. Kim, S.H. Kim, J.H. Kim, J.W. Park, Y.W. Expression profiling of cyclin B1 and D1 in cervical carcinoma Experimental Oncology |
| description |
Aim: Cyclins are a family of regulatory proteins that play a key role in controlling the cell cycle. Abnormalities of cell cycle regulators, including cyclins and cyclin dependent kinases, have been reported in various malignant tumors. This study was undertaken to quantitatively detect cyclin B1 and D1 in cervical cancer. Methods: A quantitative real-time reverse transcription polymerase chain reaction and Western blot assay were used to analyze the expression of cyclin B1/D1 mRNA and proteins, respectively, in fresh invasive cervical cancer (n = 41) and normal cervical tissues (n = 10). Results: There was significantly greater cyclin B1 expression in invasive cervical cancer than in normal cervical tissue (P = 0.019). However, cyclin D1 expression was not significantly different. A Western blot assay yielded similar results. Conclusion: Our results were consistent with the concept that up-regulation of cyclin B1 expression occurred in cervical cancer and an aberrant expression of cyclin B1 might play an important role in cervical carcinogenesis. |
| format |
Article |
| author |
Zhao, M. Kim, Y.T. Yoon, B.S. Kim, S.W. Kang, M.H. Kim, S.H. Kim, J.H. Kim, J.W. Park, Y.W. |
| author_facet |
Zhao, M. Kim, Y.T. Yoon, B.S. Kim, S.W. Kang, M.H. Kim, S.H. Kim, J.H. Kim, J.W. Park, Y.W. |
| author_sort |
Zhao, M. |
| title |
Expression profiling of cyclin B1 and D1 in cervical carcinoma |
| title_short |
Expression profiling of cyclin B1 and D1 in cervical carcinoma |
| title_full |
Expression profiling of cyclin B1 and D1 in cervical carcinoma |
| title_fullStr |
Expression profiling of cyclin B1 and D1 in cervical carcinoma |
| title_full_unstemmed |
Expression profiling of cyclin B1 and D1 in cervical carcinoma |
| title_sort |
expression profiling of cyclin b1 and d1 in cervical carcinoma |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2006 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/134157 |
| citation_txt |
Expression profiling of cyclin B1 and D1 in cervical carcinoma / M. Zhao, Y.T. Kim, // Experimental Oncology. — 2006. — Т. 28, № 1. — С. 44-48. — Бібліогр.: 23 назв. — англ. |
| series |
Experimental Oncology |
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2023-10-18T21:07:31Z |
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2023-10-18T21:07:31Z |
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