The association between CD99 and LMP-1 expression in nasopharyngeal carcinoma
Aim: To characterize the roles of LMP-1 and CD99 in nasopharyngeal carcinogenesis, we undertook this pilot study of LMP-1 and CD99 expressions in nasopharyngeal cancer (NPC). Materials and Method: 40 NPC tissue samples were grouped according to the WHO classification. Immunohistochemical studies wer...
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| Дата: | 2006 |
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| Автори: | , , , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2006
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/134158 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | The association between CD99 and LMP-1 expression in nasopharyngeal carcinoma / H.-S. Kim, J.-S. Kim, J.-T. Park, M.-C. Lee, S.-W. Juhng, J.-H. Cho, C.-S. Park // Experimental Oncology. — 2006. — Т. 28, № 1. — С. 40-43. — Бібліогр.: 18 назв. — англ. |
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irk-123456789-1341582018-06-13T03:03:36Z The association between CD99 and LMP-1 expression in nasopharyngeal carcinoma Kim, H.-S. Kim, J.-S. Park, J.-T. Lee, M.-C. Juhng, S.-W. Cho, J.-H. Park, C.-S. Original contributions Aim: To characterize the roles of LMP-1 and CD99 in nasopharyngeal carcinogenesis, we undertook this pilot study of LMP-1 and CD99 expressions in nasopharyngeal cancer (NPC). Materials and Method: 40 NPC tissue samples were grouped according to the WHO classification. Immunohistochemical studies were performed using monoclonal antibodies against EBV latent membrane protein 1 (LMP-1) and CD99 protein. In addition, CD99 expression was evaluated in 10 samples of non-neoplastic nasopharyngeal epithelium. Results: LMP-1 was detected in 12 of the 40 (30.0%) cases and its expression was found to be confined to epithelial tumor cells. WHO type I NPC samples were completely negative for LMP-1, whereas WHO type III NPC samples showed highest expression. Interestingly, CD99 was expressed in all of the non-neoplastic nasopharyngeal epithelium samples along the cytoplasmic border. CD99 expression was noted in NPC tumor cells (5 of the 40 cases, 12.5%) and in surrounding lymphoid stroma (23 of the 40 cases, 57.5%), but was not expressed in WHO type I NPC. In the 12 LMP-1 positive cases, 9 cases (75.0%) were CD99 negative, and 3 cases (25.5%) were CD99 positive. There was a statistical significance between LMP-1 and CD99 expression in lymphoid stroma. Conclusion: Our results suggest that the LMP-1 induced down-regulation of the CD99 pathway is important in nasopharyngeal carcinogenesis, and that the expression of CD99 in lymphoid stroma may regulate immune response to NPC. Цель: изучить роль экспрессии латентного мембранного белка-1 (LMP-1) EBV и CD99 при развитии карциномы носоглотки (КНГ). Материалы и методы: в соответствии с классификацией ВОЗ 40 образцов КНГ были отнесены к типам I, II, III. Иммуногистохимический анализ проводили с использованием моноклональных антител против LMP-1 и CD99. Уровень экспрессии CD99 также был определен в 10 образцах нетрансформированного эпителия носоглотки. Результаты: экспрессия белка LMP-1 была выявлена в 12 из 40 образцов КНГ (30%) только в эпителиальных клетках опухоли. В КНГ I типа все образцы были негативными по экспрессии LMP-1, а в КНГ III типа была обнаружена самая высокая степень экспрессии. Интересно, что экспрессия CD99 была выявлена во всех контрольных образцах эпителия носоглотки по краю цитоплазмы. Ее также наблюдали в опухолевых клетках (в 12,5 образцов КНГ) и в окружающей лимфоидной строме (57,5% образцов КНГ), но не выявляли в КНГ І типа. Среди 12 LMP-1 позитивных образцов было выявлено 9 CD99 негативных (75,%) и 3 — CD99 позитивных (25,5%). Также наблюдались статистически значимые различия между экспрессией LMP-1 и CD99 в лимфоидной строме. Выводы:LMP-1 индуцирует подавление каскада CD99, выполняющего важную роль в образовании опухолей носоглотки. Экспрессия CD99 в лимфоидной строме может регулировать иммунную реакцию на КНГ. 2006 Article The association between CD99 and LMP-1 expression in nasopharyngeal carcinoma / H.-S. Kim, J.-S. Kim, J.-T. Park, M.-C. Lee, S.-W. Juhng, J.-H. Cho, C.-S. Park // Experimental Oncology. — 2006. — Т. 28, № 1. — С. 40-43. — Бібліогр.: 18 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/134158 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Kim, H.-S. Kim, J.-S. Park, J.-T. Lee, M.-C. Juhng, S.-W. Cho, J.-H. Park, C.-S. The association between CD99 and LMP-1 expression in nasopharyngeal carcinoma Experimental Oncology |
| description |
Aim: To characterize the roles of LMP-1 and CD99 in nasopharyngeal carcinogenesis, we undertook this pilot study of LMP-1 and CD99 expressions in nasopharyngeal cancer (NPC). Materials and Method: 40 NPC tissue samples were grouped according to the WHO classification. Immunohistochemical studies were performed using monoclonal antibodies against EBV latent membrane protein 1 (LMP-1) and CD99 protein. In addition, CD99 expression was evaluated in 10 samples of non-neoplastic nasopharyngeal epithelium. Results: LMP-1 was detected in 12 of the 40 (30.0%) cases and its expression was found to be confined to epithelial tumor cells. WHO type I NPC samples were completely negative for LMP-1, whereas WHO type III NPC samples showed highest expression. Interestingly, CD99 was expressed in all of the non-neoplastic nasopharyngeal epithelium samples along the cytoplasmic border. CD99 expression was noted in NPC tumor cells (5 of the 40 cases, 12.5%) and in surrounding lymphoid stroma (23 of the 40 cases, 57.5%), but was not expressed in WHO type I NPC. In the 12 LMP-1 positive cases, 9 cases (75.0%) were CD99 negative, and 3 cases (25.5%) were CD99 positive. There was a statistical significance between LMP-1 and CD99 expression in lymphoid stroma. Conclusion: Our results suggest that the LMP-1 induced down-regulation of the CD99 pathway is important in nasopharyngeal carcinogenesis, and that the expression of CD99 in lymphoid stroma may regulate immune response to NPC. |
| format |
Article |
| author |
Kim, H.-S. Kim, J.-S. Park, J.-T. Lee, M.-C. Juhng, S.-W. Cho, J.-H. Park, C.-S. |
| author_facet |
Kim, H.-S. Kim, J.-S. Park, J.-T. Lee, M.-C. Juhng, S.-W. Cho, J.-H. Park, C.-S. |
| author_sort |
Kim, H.-S. |
| title |
The association between CD99 and LMP-1 expression in nasopharyngeal carcinoma |
| title_short |
The association between CD99 and LMP-1 expression in nasopharyngeal carcinoma |
| title_full |
The association between CD99 and LMP-1 expression in nasopharyngeal carcinoma |
| title_fullStr |
The association between CD99 and LMP-1 expression in nasopharyngeal carcinoma |
| title_full_unstemmed |
The association between CD99 and LMP-1 expression in nasopharyngeal carcinoma |
| title_sort |
association between cd99 and lmp-1 expression in nasopharyngeal carcinoma |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2006 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/134158 |
| citation_txt |
The association between CD99 and LMP-1 expression in nasopharyngeal carcinoma /
H.-S. Kim, J.-S. Kim, J.-T. Park, M.-C. Lee, S.-W. Juhng, J.-H. Cho, C.-S. Park // Experimental Oncology. — 2006. — Т. 28, № 1. — С. 40-43. — Бібліогр.: 18 назв. — англ. |
| series |
Experimental Oncology |
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| first_indexed |
2023-10-18T21:07:31Z |
| last_indexed |
2023-10-18T21:07:31Z |
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