Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin

Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin

Aim: To compare the molecular profile and cell cycle of sensitive and resistant to doxorubicin MCF-7 breast cancer cells upon exposition to conventional or liposome-encapsulated forms of doxorubicin. Methods: МТТ-test, immunocytochemistry, flow cytometry. Results: In sensitive MCF-7 cells the exposu...

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Дата:2009
Автори: Rusetskaya, N.V., Lukyanova, N.Yu., Chekhun, V.F.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2009
Назва видання:Experimental Oncology
Теми:
Original contributions
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/134401
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin / N.V. Rusetskaya, N.Yu. Lukyanova, V.F. Chekhun // Experimental Oncology. — 2009. — Т. 31, № 3. — С. 140-143. — Бібліогр.: 11 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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spelling irk-123456789-1344012018-06-14T03:04:52Z Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin Rusetskaya, N.V. Lukyanova, N.Yu. Chekhun, V.F. Original contributions Aim: To compare the molecular profile and cell cycle of sensitive and resistant to doxorubicin MCF-7 breast cancer cells upon exposition to conventional or liposome-encapsulated forms of doxorubicin. Methods: МТТ-test, immunocytochemistry, flow cytometry. Results: In sensitive MCF-7 cells the exposure to conventional but not liposomal form of doxorubicin decreased metallothionein (MT) expression demonstrating activation of MT-detoxification system. In doxorubicin-resistant cells (MCF-7/Dох) MT expression drastically decreased. Conventional but not liposomal form of doxorubicin reduced the levels of expression of steroid hormones receptors on MCF-7 sensitive cells. The exposure of ΜCF-7 cells to conventional form of doxorubicin resulted in the decrease of p53 expression and the increase of Bcl-2 expression. In MCF-7/Dох cells neither conventional nor liposomal form of doxorubicin altered Bcl-2 expression. The exposure of MCF-7 but not MCF-7/Dох to doxorubicin in conventional form caused cell cycle arrest in G0/G1. Upon exposure to doxorubicin in liposomal form, cell cycle blockage in G0/G1 phase was observed in both sensitive and resistant sublines. Conclusion: The differential effects of the conventional and liposomal forms of doxorubicin in sensitive and resistant cells have been demonstrated. Exposure of MCF-7 and MCF-7/Dох cells to doxorubicin in liposomal form alters the molecular profile and cell distribution over cell cycle phases. 2009 Article Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin / N.V. Rusetskaya, N.Yu. Lukyanova, V.F. Chekhun // Experimental Oncology. — 2009. — Т. 31, № 3. — С. 140-143. — Бібліогр.: 11 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/134401 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Rusetskaya, N.V.
Lukyanova, N.Yu.
Chekhun, V.F.
Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin
Experimental Oncology
description Aim: To compare the molecular profile and cell cycle of sensitive and resistant to doxorubicin MCF-7 breast cancer cells upon exposition to conventional or liposome-encapsulated forms of doxorubicin. Methods: МТТ-test, immunocytochemistry, flow cytometry. Results: In sensitive MCF-7 cells the exposure to conventional but not liposomal form of doxorubicin decreased metallothionein (MT) expression demonstrating activation of MT-detoxification system. In doxorubicin-resistant cells (MCF-7/Dох) MT expression drastically decreased. Conventional but not liposomal form of doxorubicin reduced the levels of expression of steroid hormones receptors on MCF-7 sensitive cells. The exposure of ΜCF-7 cells to conventional form of doxorubicin resulted in the decrease of p53 expression and the increase of Bcl-2 expression. In MCF-7/Dох cells neither conventional nor liposomal form of doxorubicin altered Bcl-2 expression. The exposure of MCF-7 but not MCF-7/Dох to doxorubicin in conventional form caused cell cycle arrest in G0/G1. Upon exposure to doxorubicin in liposomal form, cell cycle blockage in G0/G1 phase was observed in both sensitive and resistant sublines. Conclusion: The differential effects of the conventional and liposomal forms of doxorubicin in sensitive and resistant cells have been demonstrated. Exposure of MCF-7 and MCF-7/Dох cells to doxorubicin in liposomal form alters the molecular profile and cell distribution over cell cycle phases.
format Article
author Rusetskaya, N.V.
Lukyanova, N.Yu.
Chekhun, V.F.
author_facet Rusetskaya, N.V.
Lukyanova, N.Yu.
Chekhun, V.F.
author_sort Rusetskaya, N.V.
title Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin
title_short Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin
title_full Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin
title_fullStr Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin
title_full_unstemmed Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin
title_sort molecular profile and cell cycle in mcf-7 and mcf‑7/dox cells exposed to conventional and liposomal forms of doxorubicin
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2009
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/134401
citation_txt Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin / N.V. Rusetskaya, N.Yu. Lukyanova, V.F. Chekhun // Experimental Oncology. — 2009. — Т. 31, № 3. — С. 140-143. — Бібліогр.: 11 назв. — англ.
series Experimental Oncology
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AT chekhunvf molecularprofileandcellcycleinmcf7andmcf7doxcellsexposedtoconventionalandliposomalformsofdoxorubicin
first_indexed 2023-10-18T21:07:57Z
last_indexed 2023-10-18T21:07:57Z
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