Antineoplastic effects of nutrient mixture on Raji and Jurkat t cells: the two highly aggressive non hodgkin’s lymphoma cell lines

Antineoplastic effects of nutrient mixture on Raji and Jurkat t cells: the two highly aggressive non hodgkin’s lymphoma cell lines

Non-Hodgkin lymphomas incidence has increased more than 70% in last 25 years. Aggressiveness, higher relapse rate, and treatment complications pose significant barriers. Decreased food intake and side effects of treatments make cancer patients vulnerable to deficiency of essential nutrients such as...

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Дата:2009
Автори: Roomi, M.W., Bhanap, B.A., Roomi, N.W., Rath, M., Niedzwiecki, A.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2009
Назва видання:Experimental Oncology
Теми:
Original contributions
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/134402
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Antineoplastic effects of nutrient mixture on Raji and Jurkat t cells: the two highly aggressive non hodgkin’s lymphoma cell lines / M.W. Roomi, B.A. Bhanap, N.W. Roomi, M. Rath, A. Niedzwiecki // Experimental Oncology. — 2009. — Т. 31, № 3. — С. 149-155. — Бібліогр.: 31 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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spelling irk-123456789-1344022018-06-14T03:04:21Z Antineoplastic effects of nutrient mixture on Raji and Jurkat t cells: the two highly aggressive non hodgkin’s lymphoma cell lines Roomi, M.W. Bhanap, B.A. Roomi, N.W. Rath, M. Niedzwiecki, A. Original contributions Non-Hodgkin lymphomas incidence has increased more than 70% in last 25 years. Aggressiveness, higher relapse rate, and treatment complications pose significant barriers. Decreased food intake and side effects of treatments make cancer patients vulnerable to deficiency of essential nutrients such as vitamin C, lysine, and proline leading to the formation of weak extra cellular matrix susceptible to easy breakdown by matrix metalloproteinase enzymes. Inhibition of these enzymes has shown promise in stopping metastasis. Aim: In this study, we investigated the effects of a specific nutrient mixture, containing ascorbic acid, lysine, proline, green tea extract among others, in most aggressive forms of non-Hodgkin’s lymphoma — Burkitt’s lymphoma, and T-cell lymphoma — using Raji and Jurkat cells respectively. Methods: Nutrient mixture (NM) doses of 0, 10, 50, 100, 500, 1000 μg/ml, were used to study effects on cell proliferation, expression of matrix metalloproteinase, Matrigel™ invasion and apoptosis. Results: The results demonstrated that the dose response toxicity of the nutrient mixture on Raji cells gradually increased with increasing concentration. The nutrient mixture was non-toxic to Jurkat cells, however exhibited anti-proliferative properties at higher concentrations. Zymography demonstrated, NM had a significant inhibitory effect on matrix metalloproteinase-9 expression with total inhibition at 1000 μg/ml for Raji cells and at 500 μg/ml for Jurkat cells. The NM at 100 μg/ml completely inhibited Matrigel ™ invasion for Raji cells, and at 1000 μg/ml for Jurkat cells. After the NM challenge virtually all Raji and Jurkat cells exposed to 1000 μg/ml were in late apoptosis. Conclusion: Considering the lack of treatment options and continually increasing incidence, NM could be further explored for its therapeutic potential in Burkitt’s lymphoma and T-cell lymphoma. 2009 Article Antineoplastic effects of nutrient mixture on Raji and Jurkat t cells: the two highly aggressive non hodgkin’s lymphoma cell lines / M.W. Roomi, B.A. Bhanap, N.W. Roomi, M. Rath, A. Niedzwiecki // Experimental Oncology. — 2009. — Т. 31, № 3. — С. 149-155. — Бібліогр.: 31 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/134402 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Roomi, M.W.
Bhanap, B.A.
Roomi, N.W.
Rath, M.
Niedzwiecki, A.
Antineoplastic effects of nutrient mixture on Raji and Jurkat t cells: the two highly aggressive non hodgkin’s lymphoma cell lines
Experimental Oncology
description Non-Hodgkin lymphomas incidence has increased more than 70% in last 25 years. Aggressiveness, higher relapse rate, and treatment complications pose significant barriers. Decreased food intake and side effects of treatments make cancer patients vulnerable to deficiency of essential nutrients such as vitamin C, lysine, and proline leading to the formation of weak extra cellular matrix susceptible to easy breakdown by matrix metalloproteinase enzymes. Inhibition of these enzymes has shown promise in stopping metastasis. Aim: In this study, we investigated the effects of a specific nutrient mixture, containing ascorbic acid, lysine, proline, green tea extract among others, in most aggressive forms of non-Hodgkin’s lymphoma — Burkitt’s lymphoma, and T-cell lymphoma — using Raji and Jurkat cells respectively. Methods: Nutrient mixture (NM) doses of 0, 10, 50, 100, 500, 1000 μg/ml, were used to study effects on cell proliferation, expression of matrix metalloproteinase, Matrigel™ invasion and apoptosis. Results: The results demonstrated that the dose response toxicity of the nutrient mixture on Raji cells gradually increased with increasing concentration. The nutrient mixture was non-toxic to Jurkat cells, however exhibited anti-proliferative properties at higher concentrations. Zymography demonstrated, NM had a significant inhibitory effect on matrix metalloproteinase-9 expression with total inhibition at 1000 μg/ml for Raji cells and at 500 μg/ml for Jurkat cells. The NM at 100 μg/ml completely inhibited Matrigel ™ invasion for Raji cells, and at 1000 μg/ml for Jurkat cells. After the NM challenge virtually all Raji and Jurkat cells exposed to 1000 μg/ml were in late apoptosis. Conclusion: Considering the lack of treatment options and continually increasing incidence, NM could be further explored for its therapeutic potential in Burkitt’s lymphoma and T-cell lymphoma.
format Article
author Roomi, M.W.
Bhanap, B.A.
Roomi, N.W.
Rath, M.
Niedzwiecki, A.
author_facet Roomi, M.W.
Bhanap, B.A.
Roomi, N.W.
Rath, M.
Niedzwiecki, A.
author_sort Roomi, M.W.
title Antineoplastic effects of nutrient mixture on Raji and Jurkat t cells: the two highly aggressive non hodgkin’s lymphoma cell lines
title_short Antineoplastic effects of nutrient mixture on Raji and Jurkat t cells: the two highly aggressive non hodgkin’s lymphoma cell lines
title_full Antineoplastic effects of nutrient mixture on Raji and Jurkat t cells: the two highly aggressive non hodgkin’s lymphoma cell lines
title_fullStr Antineoplastic effects of nutrient mixture on Raji and Jurkat t cells: the two highly aggressive non hodgkin’s lymphoma cell lines
title_full_unstemmed Antineoplastic effects of nutrient mixture on Raji and Jurkat t cells: the two highly aggressive non hodgkin’s lymphoma cell lines
title_sort antineoplastic effects of nutrient mixture on raji and jurkat t cells: the two highly aggressive non hodgkin’s lymphoma cell lines
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2009
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/134402
citation_txt Antineoplastic effects of nutrient mixture on Raji and Jurkat t cells: the two highly aggressive non hodgkin’s lymphoma cell lines / M.W. Roomi, B.A. Bhanap, N.W. Roomi, M. Rath, A. Niedzwiecki // Experimental Oncology. — 2009. — Т. 31, № 3. — С. 149-155. — Бібліогр.: 31 назв. — англ.
series Experimental Oncology
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first_indexed 2023-10-18T21:07:57Z
last_indexed 2023-10-18T21:07:57Z
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