Ganoderma lucidum extract inhibits proliferation of SW 480 human colorectal cancer cells

Ganoderma lucidum extract inhibits proliferation of SW 480 human colorectal cancer cells

Aim: Ganoderma lucidum is a commonly used Chinese herb and an important ingredient in traditional Chinese medicine herbal formulations for immune dysfunction related illnesses. The effects of this medicinal mushroom on human colorectal cancer cells have not yet been evaluated. In this study, we inve...

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Бібліографічні деталі
Дата:2006
Автори: Xie, J.T., Wang, C.Z., Wicks, S., Yin, J.J., Kong, J., Li, J., Li, Y.C., Yuan, C.S.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2006
Назва видання:Experimental Oncology
Теми:
Original contributions
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/134527
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Ganoderma lucidum extract inhibits proliferation of SW 480 human colorectal cancer cells / J.T. Xie, C.Z. Wang, S. Wicks, J.J. Yin, J. Kong, J. Li, Y.C. Li, C.S. Yuan // Experimental Oncology. — 2006. — Т. 28, № 1. — С. 25-29. — Бібліогр.: 22 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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spelling irk-123456789-1345272018-06-14T03:06:46Z Ganoderma lucidum extract inhibits proliferation of SW 480 human colorectal cancer cells Xie, J.T. Wang, C.Z. Wicks, S. Yin, J.J. Kong, J. Li, J. Li, Y.C. Yuan, C.S. Original contributions Aim: Ganoderma lucidum is a commonly used Chinese herb and an important ingredient in traditional Chinese medicine herbal formulations for immune dysfunction related illnesses. The effects of this medicinal mushroom on human colorectal cancer cells have not yet been evaluated. In this study, we investigated the effects of Ganoderma lucidum extract using SW 480 human colorectal cancer cell line. Materials and Methods: Two different fractions of Ganoderma lucidum extract, i.e., a fraction containing mainly polysaccharides (GLE-1), and a triterpenoid fraction without polysaccharides (GLE-2) were analyzed. Their antiproliferative activity was evaluated by cell proliferation assay and 3H-thymidine incorporation assay. Scavenging effects of DPPH radical were assessed using ESR-spectroscopy. Results: Our data showed that both GLE-1 and GLE-2 significantly inhibited the proliferation of SW 480 cells. The inhibitory effect of GLE-2 was much stronger than that of GLE-1. GLE-1 inhibited DNA synthesis in the cells and reduced the formation of DPPH radicals. Conclusion: Ganoderma lucidum extract inhibits proliferation of human colorectal cancer cells and possesses antioxidant properties. Цель: для лечения заболеваний, связанных с нарушениями функционирования иммунной системы, в традиционной китайской медицине часто используют препараты на основе Ganoderma lucidum. В данной работе было изучено влияние экстракта Ganoderma lucidum на клетки колоректального рака человека линии SW 480. Материалы и методы: изучали две фракции экстракта Ganoderma lucidum: фракцию GLE-1, содержащую полисахариды, и тритерпеноидную фракцию GLE-2. Их рост-ингибирующую активность определяли с помощью анализа клеточной пролиферации и по включению 3 H-тимидина. Для оценки антиоксидантной активности использовали ЕСР-спектроскопию. Результаты: обе фракции Ganoderma lucidum подавляли пролиферацию клеток SW 480. Рост-ингибирующий эффект GLE-2 был более выраженным, чем фракции GLE-1. Фракция GLE-1 подавляла синтез ДНК в клетках линии SW 480 и понижала уровень формирования DPPH радикалов. Выводы: экстракт Ganoderma lucidum подавляет пролиферацию клеток колоректального рака человека и обладает антиоксидантными свойствами. 2006 Article Ganoderma lucidum extract inhibits proliferation of SW 480 human colorectal cancer cells / J.T. Xie, C.Z. Wang, S. Wicks, J.J. Yin, J. Kong, J. Li, Y.C. Li, C.S. Yuan // Experimental Oncology. — 2006. — Т. 28, № 1. — С. 25-29. — Бібліогр.: 22 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/134527 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Xie, J.T.
Wang, C.Z.
Wicks, S.
Yin, J.J.
Kong, J.
Li, J.
Li, Y.C.
Yuan, C.S.
Ganoderma lucidum extract inhibits proliferation of SW 480 human colorectal cancer cells
Experimental Oncology
description Aim: Ganoderma lucidum is a commonly used Chinese herb and an important ingredient in traditional Chinese medicine herbal formulations for immune dysfunction related illnesses. The effects of this medicinal mushroom on human colorectal cancer cells have not yet been evaluated. In this study, we investigated the effects of Ganoderma lucidum extract using SW 480 human colorectal cancer cell line. Materials and Methods: Two different fractions of Ganoderma lucidum extract, i.e., a fraction containing mainly polysaccharides (GLE-1), and a triterpenoid fraction without polysaccharides (GLE-2) were analyzed. Their antiproliferative activity was evaluated by cell proliferation assay and 3H-thymidine incorporation assay. Scavenging effects of DPPH radical were assessed using ESR-spectroscopy. Results: Our data showed that both GLE-1 and GLE-2 significantly inhibited the proliferation of SW 480 cells. The inhibitory effect of GLE-2 was much stronger than that of GLE-1. GLE-1 inhibited DNA synthesis in the cells and reduced the formation of DPPH radicals. Conclusion: Ganoderma lucidum extract inhibits proliferation of human colorectal cancer cells and possesses antioxidant properties.
format Article
author Xie, J.T.
Wang, C.Z.
Wicks, S.
Yin, J.J.
Kong, J.
Li, J.
Li, Y.C.
Yuan, C.S.
author_facet Xie, J.T.
Wang, C.Z.
Wicks, S.
Yin, J.J.
Kong, J.
Li, J.
Li, Y.C.
Yuan, C.S.
author_sort Xie, J.T.
title Ganoderma lucidum extract inhibits proliferation of SW 480 human colorectal cancer cells
title_short Ganoderma lucidum extract inhibits proliferation of SW 480 human colorectal cancer cells
title_full Ganoderma lucidum extract inhibits proliferation of SW 480 human colorectal cancer cells
title_fullStr Ganoderma lucidum extract inhibits proliferation of SW 480 human colorectal cancer cells
title_full_unstemmed Ganoderma lucidum extract inhibits proliferation of SW 480 human colorectal cancer cells
title_sort ganoderma lucidum extract inhibits proliferation of sw 480 human colorectal cancer cells
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2006
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/134527
citation_txt Ganoderma lucidum extract inhibits proliferation of SW 480 human colorectal cancer cells / J.T. Xie, C.Z. Wang, S. Wicks, J.J. Yin, J. Kong, J. Li, Y.C. Li, C.S. Yuan // Experimental Oncology. — 2006. — Т. 28, № 1. — С. 25-29. — Бібліогр.: 22 назв. — англ.
series Experimental Oncology
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first_indexed 2023-10-18T21:08:56Z
last_indexed 2023-10-18T21:08:56Z
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