Protective effect of antioxidant enzymes against drug cytotoxicity in MCF-7 cells
Aim: To evaluate protective effect of antioxidant enzymes against epirubicin-HCI (EPI) cytotoxicity in vitro. Materials and Methods: Viability of MCF-7 cells treated with EPI was measured using the MTT test. Glutathione (GSH), protein content and enzymatic activity were measured spectrophotometrical...
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| Дата: | 2006 |
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| Автори: | , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2006
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/134531 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Protective effect of antioxidant enzymes against drug cytotoxicity in MCF-7 cells / A. Ozkan, K. Fiskin // Experimental Oncology. — 2006. — Т. 28, № 1. — С. 86–88. — Бібліогр.: 15 назв. — англ. |
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irk-123456789-1345312018-06-14T03:06:43Z Protective effect of antioxidant enzymes against drug cytotoxicity in MCF-7 cells Ozkan, A. Fiskin, K. Short communications Aim: To evaluate protective effect of antioxidant enzymes against epirubicin-HCI (EPI) cytotoxicity in vitro. Materials and Methods: Viability of MCF-7 cells treated with EPI was measured using the MTT test. Glutathione (GSH), protein content and enzymatic activity were measured spectrophotometrically. NADPH — dependent cytochrome P-450 reductase (NADPH-CYP-450) and glutathione S-transferase pi (GST-pi) expression in MCF-7 cells were determined by Western blot analysis. Results: The IC50 values of EPI in MCF-7 cells were 1.0, 0.7 and 0.5 ng/ml respectively for 24, 48 and 72 h applications. Simultaneously enzymatic activity of glutathione S-transferase, glutathione peroxidase, GSH and expression of GST-pi, NADPH-CYP-450 reductase were increased in EPI (1 ng/ml) — treated cells at the end of the 24 h incubation. Addition of superoxide dismutase, catalase and GSH decreased cytotoxicity of EPI. Conclusion: We hypothesized that the production of reactive oxygen species and hydrogen peroxide as result of EPI treatment can cause cytotoxicity in MCF-7 cells and antioxidant enzymes protect the cells against this process. Цель: оценить защитный эффект антиоксидантных ферментов против цитотоксичности эпирубицина-HCI (ЕРІ) in vitro. Материалы и методы: жизнеспособность клеток линии MCF-7, обработанных ЕРІ, оценивали при помощи MTT-метода. Содержание глутатиона (GSH), концентрацию белка и ферментативную активность измеряли спектрофотометрически. Экспрессию NADPH-зависимой цитохром P-450 редуктазы (NADPH-CYP-450) и глутатион-S-трансферазы-pi в клетках линии MCF-7 определяли при помощи Вестерн блот-анализа. Результаты: значения IC50 для клеток MСF-7, обработанных EPI, составили 1,0, 0,7 и 0,5 нг/мл при 24, 48 и 72 ч инкубации соответственно. Через 24 ч инкубации клеток с 1 нг/мл ЕРІ отмечали повышение ферментативной активности глутатион-S-трансферазы и глутатион-пероксидазы, а также уровней экспрессии GST-рi и NADPH-CYP-450 редуктазы. Прединкубация клеток с супероксиддисмутазой, каталазой и GSH приводила к снижению цитотоксичности ЕРІ. Выводы: образование свободных радикалов кислорода и перекиси водорода при действии ЕРІ на клетки линии MCF-7 приводит к цитотоксическому эффекту, в снижении которого участвуют антиоксидантные ферменты. 2006 Article Protective effect of antioxidant enzymes against drug cytotoxicity in MCF-7 cells / A. Ozkan, K. Fiskin // Experimental Oncology. — 2006. — Т. 28, № 1. — С. 86–88. — Бібліогр.: 15 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/134531 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Short communications Short communications |
| spellingShingle |
Short communications Short communications Ozkan, A. Fiskin, K. Protective effect of antioxidant enzymes against drug cytotoxicity in MCF-7 cells Experimental Oncology |
| description |
Aim: To evaluate protective effect of antioxidant enzymes against epirubicin-HCI (EPI) cytotoxicity in vitro. Materials and Methods: Viability of MCF-7 cells treated with EPI was measured using the MTT test. Glutathione (GSH), protein content and enzymatic activity were measured spectrophotometrically. NADPH — dependent cytochrome P-450 reductase (NADPH-CYP-450) and glutathione S-transferase pi (GST-pi) expression in MCF-7 cells were determined by Western blot analysis. Results: The IC50 values of EPI in MCF-7 cells were 1.0, 0.7 and 0.5 ng/ml respectively for 24, 48 and 72 h applications. Simultaneously enzymatic activity of glutathione S-transferase, glutathione peroxidase, GSH and expression of GST-pi, NADPH-CYP-450 reductase were increased in EPI (1 ng/ml) — treated cells at the end of the 24 h incubation. Addition of superoxide dismutase, catalase and GSH decreased cytotoxicity of EPI. Conclusion: We hypothesized that the production of reactive oxygen species and hydrogen peroxide as result of EPI treatment can cause cytotoxicity in MCF-7 cells and antioxidant enzymes protect the cells against this process. |
| format |
Article |
| author |
Ozkan, A. Fiskin, K. |
| author_facet |
Ozkan, A. Fiskin, K. |
| author_sort |
Ozkan, A. |
| title |
Protective effect of antioxidant enzymes against drug cytotoxicity in MCF-7 cells |
| title_short |
Protective effect of antioxidant enzymes against drug cytotoxicity in MCF-7 cells |
| title_full |
Protective effect of antioxidant enzymes against drug cytotoxicity in MCF-7 cells |
| title_fullStr |
Protective effect of antioxidant enzymes against drug cytotoxicity in MCF-7 cells |
| title_full_unstemmed |
Protective effect of antioxidant enzymes against drug cytotoxicity in MCF-7 cells |
| title_sort |
protective effect of antioxidant enzymes against drug cytotoxicity in mcf-7 cells |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2006 |
| topic_facet |
Short communications |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/134531 |
| citation_txt |
Protective effect of antioxidant enzymes against drug cytotoxicity in MCF-7 cells / A. Ozkan, K. Fiskin // Experimental Oncology. — 2006. — Т. 28, № 1. — С. 86–88. — Бібліогр.: 15 назв. — англ. |
| series |
Experimental Oncology |
| work_keys_str_mv |
AT ozkana protectiveeffectofantioxidantenzymesagainstdrugcytotoxicityinmcf7cells AT fiskink protectiveeffectofantioxidantenzymesagainstdrugcytotoxicityinmcf7cells |
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2023-10-18T21:08:57Z |
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2023-10-18T21:08:57Z |
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1796152051943604224 |