Molecular profile and cell cycle in mcf-7 cells resistant to cisplatin and doxorubicin
Aim: To compare ultrastructure, phenotypic profile and cell cycle progression ofMCF-7 human breast cancer cells and MCF7 sublines resistant to cisplatin (MCF-7/DDP) and doxorubicin (MCF-7/DOX). Methods: MTT-test, immunocytochemistry, flow cytometry, electron microscopy. Results: The development of...
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| Дата: | 2009 |
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| Автори: | , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2009
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/135701 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Molecular profile and cell cycle in mcf-7 cells resistant to cisplatin and doxorubicin / N.Yu. Lukyanova, N.V. Rusetskya, N.A. Tregubova, V.F. Chekhun // Experimental Oncology. — 2009. — Т. 31, № 2. — С. 87–91. — Бібліогр.: 26 назв. — англ. |
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irk-123456789-1357012018-06-16T03:11:27Z Molecular profile and cell cycle in mcf-7 cells resistant to cisplatin and doxorubicin Lukyanova, N.Yu. Rusetskya, N.V. Tregubova, N.A. Chekhun, V.F. Original contributions Aim: To compare ultrastructure, phenotypic profile and cell cycle progression ofMCF-7 human breast cancer cells and MCF7 sublines resistant to cisplatin (MCF-7/DDP) and doxorubicin (MCF-7/DOX). Methods: MTT-test, immunocytochemistry, flow cytometry, electron microscopy. Results: The development of drug resistance to cisplatin and doxorubicin in MCF-7 cells upon the culturing of the initial cells with the raising concentrations of cytostatics was accompanied by the increase in cells adhesion, the increasing differentiation grade and the loss of steroid hormone receptors. Besides, it was shown that antiapoptotic mechanisms (decrease ofBcl-2 expression) and intracellular glutathione detoxifying system are involved in the process of cisplatin resistance development inMCF-7 cells. Atthe same time, P-glycoprotein overexpression in cells resistant to doxorubicin suggests MDR-dependent mechanism. Both doxorubicin- and cisplatin-resistant cells are characterized by the changes in the expression of several cell cycle regulators — Ki-67, cyclin D1, pRb and р21). Conclusion: The long-time culture of MCF-7 cells with cytostatic drugs results in the decreased cyclin D1, pRb, and Ki-67 expression and increased р21 expression with the increasing differentiation grade of the resistant cells. The underlying mechanisms of resistance to cisplatin and doxorubicin in MCF-7 cells may be different. 2009 Article Molecular profile and cell cycle in mcf-7 cells resistant to cisplatin and doxorubicin / N.Yu. Lukyanova, N.V. Rusetskya, N.A. Tregubova, V.F. Chekhun // Experimental Oncology. — 2009. — Т. 31, № 2. — С. 87–91. — Бібліогр.: 26 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/135701 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Lukyanova, N.Yu. Rusetskya, N.V. Tregubova, N.A. Chekhun, V.F. Molecular profile and cell cycle in mcf-7 cells resistant to cisplatin and doxorubicin Experimental Oncology |
| description |
Aim: To compare ultrastructure, phenotypic profile and cell cycle progression ofMCF-7 human breast cancer cells and MCF7 sublines
resistant to cisplatin (MCF-7/DDP) and doxorubicin (MCF-7/DOX). Methods: MTT-test, immunocytochemistry, flow
cytometry, electron microscopy. Results: The development of drug resistance to cisplatin and doxorubicin in MCF-7 cells upon the
culturing of the initial cells with the raising concentrations of cytostatics was accompanied by the increase in cells adhesion, the
increasing differentiation grade and the loss of steroid hormone receptors. Besides, it was shown that antiapoptotic mechanisms
(decrease ofBcl-2 expression) and intracellular glutathione detoxifying system are involved in the process of cisplatin resistance development
inMCF-7 cells. Atthe same time, P-glycoprotein overexpression in cells resistant to doxorubicin suggests MDR-dependent
mechanism. Both doxorubicin- and cisplatin-resistant cells are characterized by the changes in the expression of several cell cycle
regulators — Ki-67, cyclin D1, pRb and р21). Conclusion: The long-time culture of MCF-7 cells with cytostatic drugs results
in the decreased cyclin D1, pRb, and Ki-67 expression and increased р21 expression with the increasing differentiation grade of the
resistant cells. The underlying mechanisms of resistance to cisplatin and doxorubicin in MCF-7 cells may be different. |
| format |
Article |
| author |
Lukyanova, N.Yu. Rusetskya, N.V. Tregubova, N.A. Chekhun, V.F. |
| author_facet |
Lukyanova, N.Yu. Rusetskya, N.V. Tregubova, N.A. Chekhun, V.F. |
| author_sort |
Lukyanova, N.Yu. |
| title |
Molecular profile and cell cycle in mcf-7 cells resistant to cisplatin and doxorubicin |
| title_short |
Molecular profile and cell cycle in mcf-7 cells resistant to cisplatin and doxorubicin |
| title_full |
Molecular profile and cell cycle in mcf-7 cells resistant to cisplatin and doxorubicin |
| title_fullStr |
Molecular profile and cell cycle in mcf-7 cells resistant to cisplatin and doxorubicin |
| title_full_unstemmed |
Molecular profile and cell cycle in mcf-7 cells resistant to cisplatin and doxorubicin |
| title_sort |
molecular profile and cell cycle in mcf-7 cells resistant to cisplatin and doxorubicin |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2009 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/135701 |
| citation_txt |
Molecular profile and cell cycle in mcf-7 cells resistant to cisplatin and doxorubicin / N.Yu. Lukyanova, N.V. Rusetskya, N.A. Tregubova, V.F. Chekhun // Experimental Oncology. — 2009. — Т. 31, № 2. — С. 87–91. — Бібліогр.: 26 назв. — англ. |
| series |
Experimental Oncology |
| work_keys_str_mv |
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| first_indexed |
2023-10-18T21:13:09Z |
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2023-10-18T21:13:09Z |
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