Application of DNA from mushroom Pleurotus ostreatus for cancer biotherapy: a pilot study
Aim: In present work, the attempt to study immunomodulatory activity and biotherapeutical potential of DNA isolated from the fruit body of P. ostreatus was made. Methods: The efficacy of biotherapeutic application of mushroom DNA was evaluated on the BALB/c mice with subcutaneously transplanted Ehrl...
Збережено в:
| Дата: | 2006 |
|---|---|
| Автори: | , , |
| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2006
|
| Назва видання: | Experimental Oncology |
| Теми: | |
| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/137562 |
| Теги: |
Додати тег
Немає тегів, Будьте першим, хто поставить тег для цього запису!
|
| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Application of DNA from mushroom Pleurotus ostreatus for cancer biotherapy: a pilot study / V.A. Shlyakhovenko, V. Kosak, S. Olishevsky // Experimental Oncology. — 2006. — Т. 28, № 2. — С. 132-135. — Бібліогр.: 28 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| id |
irk-123456789-137562 |
|---|---|
| record_format |
dspace |
| spelling |
irk-123456789-1375622018-06-18T03:02:56Z Application of DNA from mushroom Pleurotus ostreatus for cancer biotherapy: a pilot study Shlyakhovenko, V.A. Kosak, V. Olishevsky, S. Original contributions Aim: In present work, the attempt to study immunomodulatory activity and biotherapeutical potential of DNA isolated from the fruit body of P. ostreatus was made. Methods: The efficacy of biotherapeutic application of mushroom DNA was evaluated on the BALB/c mice with subcutaneously transplanted Ehrlich carcinoma. The effect of Pleurotus ostreatus DNA on NK activity was studied in vitro using nonspecific cytotoxicity assay. Results: Application of mushroom DNA resulted in augmentation of NK cytotoxic activity in vitro and significant increase of the life span of mice with solid Ehrlich carcinoma. Conclusion: The observed effects of P. ostreatus DNA administration can be probably explained by the presence of immunostimulatory unmethylated CpG motifs in DNA. Цель: в данной работе сделана попытка исследовать иммуномодулирующее влияние ДНК из плодового тела гриба Pleurotus ostreatus на активность NK-клеток, а также возможность ее применения в экспериментальной биотерапии опухолей. Методы: эффективность терапевтического применения грибной ДНК изучали у мышей линии BALB/c с подкожно трансплантированной карциномой Эрлиха. Влияние ДНК Р. ostreatus на активность NK-клеток исследовали in vitro с помощью теста неспеинфической цитотоксичности. Результаты: установлено, что интраперитонеальное введение ДНК повышает цитотоксическую активность NK-клеток in vitro и значительно повышает выживаемость мышей с солидной карциномой Эрлиха. Выводы: наблюдаемые эффекты, вероятно, можно объяснить наличием в составе ДНК из Р ostreatus иммуностимулирующих неметилированных CpG-мотивов. 2006 Article Application of DNA from mushroom Pleurotus ostreatus for cancer biotherapy: a pilot study / V.A. Shlyakhovenko, V. Kosak, S. Olishevsky // Experimental Oncology. — 2006. — Т. 28, № 2. — С. 132-135. — Бібліогр.: 28 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/137562 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Shlyakhovenko, V.A. Kosak, V. Olishevsky, S. Application of DNA from mushroom Pleurotus ostreatus for cancer biotherapy: a pilot study Experimental Oncology |
| description |
Aim: In present work, the attempt to study immunomodulatory activity and biotherapeutical potential of DNA isolated from the fruit body of P. ostreatus was made. Methods: The efficacy of biotherapeutic application of mushroom DNA was evaluated on the BALB/c mice with subcutaneously transplanted Ehrlich carcinoma. The effect of Pleurotus ostreatus DNA on NK activity was studied in vitro using nonspecific cytotoxicity assay. Results: Application of mushroom DNA resulted in augmentation of NK cytotoxic activity in vitro and significant increase of the life span of mice with solid Ehrlich carcinoma. Conclusion: The observed effects of P. ostreatus DNA administration can be probably explained by the presence of immunostimulatory unmethylated CpG motifs in DNA. |
| format |
Article |
| author |
Shlyakhovenko, V.A. Kosak, V. Olishevsky, S. |
| author_facet |
Shlyakhovenko, V.A. Kosak, V. Olishevsky, S. |
| author_sort |
Shlyakhovenko, V.A. |
| title |
Application of DNA from mushroom Pleurotus ostreatus for cancer biotherapy: a pilot study |
| title_short |
Application of DNA from mushroom Pleurotus ostreatus for cancer biotherapy: a pilot study |
| title_full |
Application of DNA from mushroom Pleurotus ostreatus for cancer biotherapy: a pilot study |
| title_fullStr |
Application of DNA from mushroom Pleurotus ostreatus for cancer biotherapy: a pilot study |
| title_full_unstemmed |
Application of DNA from mushroom Pleurotus ostreatus for cancer biotherapy: a pilot study |
| title_sort |
application of dna from mushroom pleurotus ostreatus for cancer biotherapy: a pilot study |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2006 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/137562 |
| citation_txt |
Application of DNA from mushroom Pleurotus ostreatus for cancer biotherapy: a pilot study /
V.A. Shlyakhovenko, V. Kosak, S. Olishevsky // Experimental Oncology. — 2006. — Т. 28, № 2. — С. 132-135. — Бібліогр.: 28 назв. — англ. |
| series |
Experimental Oncology |
| work_keys_str_mv |
AT shlyakhovenkova applicationofdnafrommushroompleurotusostreatusforcancerbiotherapyapilotstudy AT kosakv applicationofdnafrommushroompleurotusostreatusforcancerbiotherapyapilotstudy AT olishevskys applicationofdnafrommushroompleurotusostreatusforcancerbiotherapyapilotstudy |
| first_indexed |
2023-10-18T21:12:51Z |
| last_indexed |
2023-10-18T21:12:51Z |
| _version_ |
1796152227156459520 |