Cross-resistance to cytosine arabinoside in human acute myeloid leukemia cells selected for resistance to vincristine
Aim: The goals of the study were to reveal the involvement of P-glycoprotein (P-gp), the product of multidrug resistance 1 gene (MDR1) in cellular resistance to vincristine (VCR) and investigate cross-resistance against cytosine arabinoside (Ara-C) in HL60 and HL60/VCR cell lines. Methods: HL60 cell...
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| Дата: | 2006 |
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| Автори: | , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2006
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/137564 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Cross-resistance to cytosine arabinoside in human acute myeloid leukemia cells selected for resistance to vincristine / U. Gunduz, Y. Baran, A.U. Uralsup // Experimental Oncology. — 2006. — Т. 28, № 2. — С. 163-165. — Бібліогр.: 16 назв. — англ. |
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irk-123456789-1375642018-06-18T03:03:57Z Cross-resistance to cytosine arabinoside in human acute myeloid leukemia cells selected for resistance to vincristine Gunduz, U. Baran, Y. Uralsup, A.U. Short communications Aim: The goals of the study were to reveal the involvement of P-glycoprotein (P-gp), the product of multidrug resistance 1 gene (MDR1) in cellular resistance to vincristine (VCR) and investigate cross-resistance against cytosine arabinoside (Ara-C) in HL60 and HL60/VCR cell lines. Methods: HL60 cells (human acute myeloid leukemia cell line) were cultured on medium with 1–50 nM of VCR for 4–6 weeks, and VCR resistant cells (HL60/VCR) were selected. The viability of cells was assessed by MTT assay and the expression of MDR1 gene was detected by RT-PCR. Results: No expression of MDR1 gene was revealed in HL60 cells, but MDR1 started to be expressed after incubation of cells with 2 nM of VCR and its expression level elevates with increase of agent concentration. MTT test has shown that HL-60/VCR cells were 75-fold more resistant to VCR and 42-fold higher resistant to cytosine arabinoside (Ara-C) compared to sensitive HL60 cells. Conclusion: Aquired resistance to VCR and cross-resistance to Ara-C correlates with MDR1 gene expression in vitro. Целью работы было исследование участия Р-гликопротеина (Р-gp), продукта гена-1 множественной лекарственной устойчивости (MDR1) в устойчивости клеток лейкемии человека к винкристину (ВКР) и перекрестной резистентности к цитозинарабинозиду (Аrа-C). Методы: клетки острой миелоидной лейкемии линии HL60 культивировали в среде с 1–50 и ВКР в течение 4–6 нед, после чего были отселектированы ВКР-резистентные клетки (HL60/ВКР). Влияние препаратов на жизнеспособность клеток изучали при помощи МТТ-метода, экспресию гена MDR — методом полимеразной цепной реакции обратной транскрипции. Результаты: в клетках линии HL60 экспрессия гена MDR не была выявлена, но была зарегистрирована после инкубации клеток с 2 нM ВКР, причем ее уровень возрастал по мере повышения концентрации ВКР в среде инкубации. Показано, что устойчивость клеток линии HL-60/ВКР к ВКР и Аrа-C в 75 и в 42 раза выше, соотвественно, чем таковая клеток линии HL60. Выводы: приобретенная устойчивость к ВКР и перекрестная устойчивость к Аrа-C коррелирует с уровнем экспрессии гена MDR . 2006 Article Cross-resistance to cytosine arabinoside in human acute myeloid leukemia cells selected for resistance to vincristine / U. Gunduz, Y. Baran, A.U. Uralsup // Experimental Oncology. — 2006. — Т. 28, № 2. — С. 163-165. — Бібліогр.: 16 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/137564 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Short communications Short communications |
| spellingShingle |
Short communications Short communications Gunduz, U. Baran, Y. Uralsup, A.U. Cross-resistance to cytosine arabinoside in human acute myeloid leukemia cells selected for resistance to vincristine Experimental Oncology |
| description |
Aim: The goals of the study were to reveal the involvement of P-glycoprotein (P-gp), the product of multidrug resistance 1 gene (MDR1) in cellular resistance to vincristine (VCR) and investigate cross-resistance against cytosine arabinoside (Ara-C) in HL60 and HL60/VCR cell lines. Methods: HL60 cells (human acute myeloid leukemia cell line) were cultured on medium with 1–50 nM of VCR for 4–6 weeks, and VCR resistant cells (HL60/VCR) were selected. The viability of cells was assessed by MTT assay and the expression of MDR1 gene was detected by RT-PCR. Results: No expression of MDR1 gene was revealed in HL60 cells, but MDR1 started to be expressed after incubation of cells with 2 nM of VCR and its expression level elevates with increase of agent concentration. MTT test has shown that HL-60/VCR cells were 75-fold more resistant to VCR and 42-fold higher resistant to cytosine arabinoside (Ara-C) compared to sensitive HL60 cells. Conclusion: Aquired resistance to VCR and cross-resistance to Ara-C correlates with MDR1 gene expression in vitro. |
| format |
Article |
| author |
Gunduz, U. Baran, Y. Uralsup, A.U. |
| author_facet |
Gunduz, U. Baran, Y. Uralsup, A.U. |
| author_sort |
Gunduz, U. |
| title |
Cross-resistance to cytosine arabinoside in human acute myeloid leukemia cells selected for resistance to vincristine |
| title_short |
Cross-resistance to cytosine arabinoside in human acute myeloid leukemia cells selected for resistance to vincristine |
| title_full |
Cross-resistance to cytosine arabinoside in human acute myeloid leukemia cells selected for resistance to vincristine |
| title_fullStr |
Cross-resistance to cytosine arabinoside in human acute myeloid leukemia cells selected for resistance to vincristine |
| title_full_unstemmed |
Cross-resistance to cytosine arabinoside in human acute myeloid leukemia cells selected for resistance to vincristine |
| title_sort |
cross-resistance to cytosine arabinoside in human acute myeloid leukemia cells selected for resistance to vincristine |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2006 |
| topic_facet |
Short communications |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/137564 |
| citation_txt |
Cross-resistance to cytosine arabinoside in human acute myeloid leukemia cells selected for resistance to vincristine / U. Gunduz, Y. Baran, A.U. Uralsup // Experimental Oncology. — 2006. — Т. 28, № 2. — С. 163-165. — Бібліогр.: 16 назв. — англ. |
| series |
Experimental Oncology |
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2023-10-18T21:14:20Z |
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2023-10-18T21:14:20Z |
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