Modulation of ROS/NO production by murine peritoneal macrophages in response to bacterial CPG DNA stimulation
Aim: To investigate the features of metabolic activation induced by bacterial CpG DNA (bCpG DNA) in peritoneal macrophages (PMjs). Methods: Electron paramagnetic resonance spin-trapping technique using respective spin traps was applied to study the generation rate of reactive oxygen species and NO p...
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| Дата: | 2006 |
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| Автори: | , , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2006
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/137566 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Modulation of ROS/NO production by murine peritoneal macrophages in response to bacterial CPG DNA stimulation / S. Olishevsky, A.P. Burlaka, E. Sidorik, V.A. Shlyakhovenko, Yu. Garpenko, V. Kozak // Experimental Oncology. — 2006. — Т. 28, № 2. — С. 114-120. — Бібліогр.: 46 назв. — англ. |
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irk-123456789-1375662018-06-18T03:04:11Z Modulation of ROS/NO production by murine peritoneal macrophages in response to bacterial CPG DNA stimulation Olishevsky, S. Burlaka, Burlaka A.P. Sidorik, E. Shlyakhovenko, V.A. Garpenko, Yu. Kozak, V. Original contributions Aim: To investigate the features of metabolic activation induced by bacterial CpG DNA (bCpG DNA) in peritoneal macrophages (PMjs). Methods: Electron paramagnetic resonance spin-trapping technique using respective spin traps was applied to study the generation rate of reactive oxygen species and NO production by PMjs of BALB/c mice. Results: For the first time the capability of bCpG DNA isolated from Bacillus subtilis GP1-807-03 culture medium to elevate activity of NADP·H oxidase and inducible NO synthase in PMjs of normal and tumor-bearing mice have been demonstrated. The main differences in superoxide anion generation rate and production of NO by PMjs of normal mice and mice with transplanted solid Ehrlich carcinoma were showed. The effects of bCpG DNA stimulation in vitro on ROS and NO production by PMjs depended on concentration and time exposure with bCpG DNA. Furthermore, response of PMjs from tumor-bearing mice on bCpG DNA stimulation was delayed as compared to PMjs of normal mice. Conclusion: The present findings suggest that bCpG DNA have modulatory effect on ROS/NO production by PMjs from normal and tumor-bearing animals. Цель: изучить особенности метаболической активации перитонеальных макрофагов (ПМФ) в ответ на воздействие, вызванное CpG ДНК бактериального происхождения. Методы: для исследования скорости генерирования радикальных форм кислорода (РФК) и продукции оксида азота (NO) ПМФ мышей линии BALB/c был применен метод электронного парамагнитного резонанса с использованием спиновых ловушек. Результаты: впервые в эксперименте продемонстрирована способность бактериальной CpG ДНК, выделенной из культуральной жидкости Bacillus subtilis GP1-807-03, повышать активность НАДФ·Н-оксидазы и индуцибельной NO-синтазы ПМФ у мышей с солидной карциномой Эрлиха и интактных мышей. Отмечены различия в скорости генерирования супероксидных радикал-анионов и уровне продуцирования NO ПМФ у животных с карциномой Эрлиха по сравнению с контрольными животными, что выражалось в замедлении ответа на воздействие бактериальной CpG ДНК. Выявлена концентрационная и временная зависимость влияния бактериальной CpG ДНК in vitro на продукцию РФК и NO ПМФ. Выводы: полученные данные свидетельствуют о том, что бактериальная CpG ДНК обладает модулирующим влиянием на продукцию РФК и NO ПМФ in vitro и in vivo. 2006 Article Modulation of ROS/NO production by murine peritoneal macrophages in response to bacterial CPG DNA stimulation / S. Olishevsky, A.P. Burlaka, E. Sidorik, V.A. Shlyakhovenko, Yu. Garpenko, V. Kozak // Experimental Oncology. — 2006. — Т. 28, № 2. — С. 114-120. — Бібліогр.: 46 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/137566 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Olishevsky, S. Burlaka, Burlaka A.P. Sidorik, E. Shlyakhovenko, V.A. Garpenko, Yu. Kozak, V. Modulation of ROS/NO production by murine peritoneal macrophages in response to bacterial CPG DNA stimulation Experimental Oncology |
| description |
Aim: To investigate the features of metabolic activation induced by bacterial CpG DNA (bCpG DNA) in peritoneal macrophages (PMjs). Methods: Electron paramagnetic resonance spin-trapping technique using respective spin traps was applied to study the generation rate of reactive oxygen species and NO production by PMjs of BALB/c mice. Results: For the first time the capability of bCpG DNA isolated from Bacillus subtilis GP1-807-03 culture medium to elevate activity of NADP·H oxidase and inducible NO synthase in PMjs of normal and tumor-bearing mice have been demonstrated. The main differences in superoxide anion generation rate and production of NO by PMjs of normal mice and mice with transplanted solid Ehrlich carcinoma were showed. The effects of bCpG DNA stimulation in vitro on ROS and NO production by PMjs depended on concentration and time exposure with bCpG DNA. Furthermore, response of PMjs from tumor-bearing mice on bCpG DNA stimulation was delayed as compared to PMjs of normal mice. Conclusion: The present findings suggest that bCpG DNA have modulatory effect on ROS/NO production by PMjs from normal and tumor-bearing animals. |
| format |
Article |
| author |
Olishevsky, S. Burlaka, Burlaka A.P. Sidorik, E. Shlyakhovenko, V.A. Garpenko, Yu. Kozak, V. |
| author_facet |
Olishevsky, S. Burlaka, Burlaka A.P. Sidorik, E. Shlyakhovenko, V.A. Garpenko, Yu. Kozak, V. |
| author_sort |
Olishevsky, S. |
| title |
Modulation of ROS/NO production by murine peritoneal macrophages in response to bacterial CPG DNA stimulation |
| title_short |
Modulation of ROS/NO production by murine peritoneal macrophages in response to bacterial CPG DNA stimulation |
| title_full |
Modulation of ROS/NO production by murine peritoneal macrophages in response to bacterial CPG DNA stimulation |
| title_fullStr |
Modulation of ROS/NO production by murine peritoneal macrophages in response to bacterial CPG DNA stimulation |
| title_full_unstemmed |
Modulation of ROS/NO production by murine peritoneal macrophages in response to bacterial CPG DNA stimulation |
| title_sort |
modulation of ros/no production by murine peritoneal macrophages in response to bacterial cpg dna stimulation |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2006 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/137566 |
| citation_txt |
Modulation of ROS/NO production by murine peritoneal macrophages in response to bacterial CPG DNA stimulation / S. Olishevsky, A.P. Burlaka, E. Sidorik, V.A. Shlyakhovenko,
Yu. Garpenko, V. Kozak // Experimental Oncology. — 2006. — Т. 28, № 2. — С. 114-120. — Бібліогр.: 46 назв. — англ. |
| series |
Experimental Oncology |
| work_keys_str_mv |
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| first_indexed |
2023-10-18T21:12:51Z |
| last_indexed |
2023-10-18T21:12:51Z |
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