Differentiating effect of thalidomide and GM-CSF combination on HL-60 acute promyelocytic leukemia cells
Aim: To investigate whether granulocyte-macrophage colony-stimulating factor (GM-CSF) with or without thalidomide can induce apoptosis and differentiation of HL-60 acute promyelocytic leukemia cell line in vitro. Methods: Effect of GM-CSF and thalidomide on proliferation of HL-60 cells was evaluated...
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| Дата: | 2006 |
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| Автори: | , , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2006
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/137571 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Differentiating effect of thalidomide and GM-CSF combination on HL-60 acute promyelocytic leukemia cells / A.U. Ural, F. Avcu, M. Zerman, M.I. Yilmaz, A. Pekel, M. Beyzadeoglu // Experimental Oncology. — 2006. — Т. 28, № 3. — С. 216-219. — Бібліогр.: 16 назв. — англ. |
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irk-123456789-1375712018-06-18T03:03:55Z Differentiating effect of thalidomide and GM-CSF combination on HL-60 acute promyelocytic leukemia cells Ural, A.U. Avcu, F. Zerman, M. Yilmaz, M.I. Pekel, A. Beyzadeoglu, M. Original contributions Aim: To investigate whether granulocyte-macrophage colony-stimulating factor (GM-CSF) with or without thalidomide can induce apoptosis and differentiation of HL-60 acute promyelocytic leukemia cell line in vitro. Methods: Effect of GM-CSF and thalidomide on proliferation of HL-60 cells was evaluated by MTT assay, cell cycle analysis was performed by propidium iodide staining approach and flow cytometry, and apoptosis rate was analyzed using FITC-conjugated annexin-V and FACScan flow cytometry. Results: The study revealed that thalidomide alone at high concentrations inhibited HL-60 cell growth and induced apoptosis. Three days treatment of low-dose thalidomide in combination with GM-CSF induced marked terminal differentiation of HL-60 cells, as it was assessed by increased expression of differentiation antigens on cell surface. Conclusion: Treatment of HL-60 cells by low concentration of thalidomide combined with GM-CSF induced terminal differentiation of HL60 cells in vitro, which may be advantageous for the elaboration of novel therapeutic regimens in patients with differentiation-inducible leukemias. Цель: изучить эффект гранулоцитарно-макрофагального колониестимулирующего фактора (ГМ-КСФ) в сочетании с талидомидом на индукцию апоптоза и дифференцировку клеток острого промиелоцитарного лейкоза линии HL-60 in vitro. Методы: для оценки пролиферации и жизнеспособности клеток HL-60 применяли MTT анализ, для изучения клеточного цикла — окраску пропидиум бромидом и проточную цитометрию. Для оценки апоптоза клетки линии HL-60 обрабатывали талидомидом, ГМ-КСФ, и совместно талидомидом и ГМ-КСФ в течении 48 ч, и затем метили анексином, конъюгированным с FITC, и анализировали с помощью проточной цитометрии. Результаты: талидомид в высоких концентрациях ингибирует пролиферацию клеток HL-60 и вызывает апоптоз. В сочетании с ГМ-КСФ в течение 3 дней талидомид в низкой концентрации индуцировал терминальную дифференцировку клеток HL-60, о чем свидетельствовало появление экспрессии дифференцировочных антигенов на поверхности клеток. Выводы: применение талидомида в низкой концентрации в сочетании с ГМ-КСФ вызывает терминальную дифференцировку клеток HL-60. 2006 Article Differentiating effect of thalidomide and GM-CSF combination on HL-60 acute promyelocytic leukemia cells / A.U. Ural, F. Avcu, M. Zerman, M.I. Yilmaz, A. Pekel, M. Beyzadeoglu // Experimental Oncology. — 2006. — Т. 28, № 3. — С. 216-219. — Бібліогр.: 16 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/137571 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Ural, A.U. Avcu, F. Zerman, M. Yilmaz, M.I. Pekel, A. Beyzadeoglu, M. Differentiating effect of thalidomide and GM-CSF combination on HL-60 acute promyelocytic leukemia cells Experimental Oncology |
| description |
Aim: To investigate whether granulocyte-macrophage colony-stimulating factor (GM-CSF) with or without thalidomide can induce apoptosis and differentiation of HL-60 acute promyelocytic leukemia cell line in vitro. Methods: Effect of GM-CSF and thalidomide on proliferation of HL-60 cells was evaluated by MTT assay, cell cycle analysis was performed by propidium iodide staining approach and flow cytometry, and apoptosis rate was analyzed using FITC-conjugated annexin-V and FACScan flow cytometry. Results: The study revealed that thalidomide alone at high concentrations inhibited HL-60 cell growth and induced apoptosis. Three days treatment of low-dose thalidomide in combination with GM-CSF induced marked terminal differentiation of HL-60 cells, as it was assessed by increased expression of differentiation antigens on cell surface. Conclusion: Treatment of HL-60 cells by low concentration of thalidomide combined with GM-CSF induced terminal differentiation of HL60 cells in vitro, which may be advantageous for the elaboration of novel therapeutic regimens in patients with differentiation-inducible leukemias. |
| format |
Article |
| author |
Ural, A.U. Avcu, F. Zerman, M. Yilmaz, M.I. Pekel, A. Beyzadeoglu, M. |
| author_facet |
Ural, A.U. Avcu, F. Zerman, M. Yilmaz, M.I. Pekel, A. Beyzadeoglu, M. |
| author_sort |
Ural, A.U. |
| title |
Differentiating effect of thalidomide and GM-CSF combination on HL-60 acute promyelocytic leukemia cells |
| title_short |
Differentiating effect of thalidomide and GM-CSF combination on HL-60 acute promyelocytic leukemia cells |
| title_full |
Differentiating effect of thalidomide and GM-CSF combination on HL-60 acute promyelocytic leukemia cells |
| title_fullStr |
Differentiating effect of thalidomide and GM-CSF combination on HL-60 acute promyelocytic leukemia cells |
| title_full_unstemmed |
Differentiating effect of thalidomide and GM-CSF combination on HL-60 acute promyelocytic leukemia cells |
| title_sort |
differentiating effect of thalidomide and gm-csf combination on hl-60 acute promyelocytic leukemia cells |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2006 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/137571 |
| citation_txt |
Differentiating effect of thalidomide and GM-CSF combination on HL-60 acute promyelocytic leukemia cells / A.U. Ural, F. Avcu, M. Zerman, M.I. Yilmaz, A. Pekel, M. Beyzadeoglu // Experimental Oncology. — 2006. — Т. 28, № 3. — С. 216-219. — Бібліогр.: 16 назв. — англ. |
| series |
Experimental Oncology |
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2023-10-18T21:14:46Z |
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2023-10-18T21:14:46Z |
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