Expression of clusterin, XIAP and survivin, and their changes by camptothecin (CPT) treatment in CPT resistant PC-3 and CPT-sensitive LNCaP cells

Expression of clusterin, XIAP and survivin, and their changes by camptothecin (CPT) treatment in CPT resistant PC-3 and CPT-sensitive LNCaP cells

Aim: Clusterin and IAPs (inhibitor of apoptosis proteins), such as survivin and XIAP, are known to be related to chemo-resistance in several cancer cells. In the current study, we investigated their expression levels in human prostate cancer cell lines, LNCaP and PC-3 which are sensitive and resista...

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Видавець:Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Дата:2006
Автори: Mizutani, K., Matsumoto, K., Hasegawa, N., Deguchi, T., Nozawa, Y.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2006
Назва видання:Experimental Oncology
Теми:
Original contributions
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/137572
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Цитувати:Expression of clusterin, XIAP and survivin, and their changes by camptothecin (CPT) treatment in CPT resistant PC-3 and CPT-sensitive LNCaP cells / K. Mizutani, K. Matsumoto, N. Hasegawa, T. Deguchi, Y. Nozawa // Experimental Oncology. — 2006. — Т. 28, № 3. — С. 209-215. — Бібліогр.: 26 назв. — англ.

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spelling irk-123456789-1375722018-06-18T03:05:39Z Expression of clusterin, XIAP and survivin, and their changes by camptothecin (CPT) treatment in CPT resistant PC-3 and CPT-sensitive LNCaP cells Mizutani, K. Matsumoto, K. Hasegawa, N. Deguchi, T. Nozawa, Y. Original contributions Aim: Clusterin and IAPs (inhibitor of apoptosis proteins), such as survivin and XIAP, are known to be related to chemo-resistance in several cancer cells. In the current study, we investigated their expression levels in human prostate cancer cell lines, LNCaP and PC-3 which are sensitive and resistant to camptothecin (CPT), topoisomerase I inhibitor, respectively. Methods: LNCaP and PC-3 cells were cultured in the presence of CPT, cell death was evaluated using Hoechst 33342 and propidium iodide (PI) double staining. The expression of clusterin, XIAP and survivin on mRNA and protein levels was investigated by semi-quantitative RT-PCR and Western blotting, respectively. Results: Our data showed that 24 h treatment of LNCaP cells with 0.5 and 3.0 µM CPT resulted in higher number of apoptotic cells, than that in PC-3 cells. Western blot analysis revealed that the clusterin level in PC-3 cells was 5-fold higher than that in LNCaP cells. In contrast, XIAP expression level in PC-3 cells was lower than that in LNCaP cells, and survivin levels were similar in these two cell lines. Treatment with 0.5 and 3.0 µM CPT resulted in the reduced survivin and XIAP expression in both cell lines, while clusterin expression remained unchanged in LNCaP cells, but was increased in PC-3 cells. Conclusion: The results suggest that clusterin may take a greater part in CPT-resistance than survivin and XIAP in PC-3 cells. Цель: известно, что кластерин и белки-ингибиторы апоптоза семейства IAP, такие как сурвивин и XIAP, связаны с химиорезистентностью опухолевых клеток. Задача исследования — определить уровни экспрессии указанных белков в линиях опухолевых клеток предстательной железы, чувствительных (LNCaP) и устойчивых (PC-3) к действию камптотецина (CPT) — ингибитора топоизомеразы I. Методы: клетки LNCaP и PC-3 инкубировали с CPT, после чего анализировали количество погибших клеток с использованием двойного окрашивания реактивом Херста 33 342 и пропидиумиодидом. Экспрессию кластерина, XIAP и сурвивина на уровне белка и мРНК оценивали методами Вестерн-блоттинга и полуколичественного РТ-ПЦР соответственно. Результаты: установлено, что вследствие 24-часовой инкубации клеток с 0,5 и 3,0 µM CPT определяют более значительный процент гибели клеток линии LNCaP, чем таковых PC-3. Данные Вестерн блот анализа выявили, что в клетках линии PC-3 количество кластерина в 5 раз больше, а XIAP — меньше, чем в клетках линии LNCaP, в то время как экспрессия сурвивина одинакова в обеих клеточных линиях. Инкубация клеток с 0,5 и 3,0 µM CPT приводила к снижению уровня сурвивина и XIAP в обеих линиях. Экспрессия кластерина на уровне мРНК и белка не изменялась при действии CPT на клетки линии LNCaP, однако повышалась в клетках линии PC-3. Выводы: полученные данные свидетельствуют о том, что в развитии резистентности к камптотецину клеток линии PC-3 кластерин может играть более важную роль, чем сурвивин и XIAP. 2006 Article Expression of clusterin, XIAP and survivin, and their changes by camptothecin (CPT) treatment in CPT resistant PC-3 and CPT-sensitive LNCaP cells / K. Mizutani, K. Matsumoto, N. Hasegawa, T. Deguchi, Y. Nozawa // Experimental Oncology. — 2006. — Т. 28, № 3. — С. 209-215. — Бібліогр.: 26 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/137572 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Mizutani, K.
Matsumoto, K.
Hasegawa, N.
Deguchi, T.
Nozawa, Y.
Expression of clusterin, XIAP and survivin, and their changes by camptothecin (CPT) treatment in CPT resistant PC-3 and CPT-sensitive LNCaP cells
Experimental Oncology
description Aim: Clusterin and IAPs (inhibitor of apoptosis proteins), such as survivin and XIAP, are known to be related to chemo-resistance in several cancer cells. In the current study, we investigated their expression levels in human prostate cancer cell lines, LNCaP and PC-3 which are sensitive and resistant to camptothecin (CPT), topoisomerase I inhibitor, respectively. Methods: LNCaP and PC-3 cells were cultured in the presence of CPT, cell death was evaluated using Hoechst 33342 and propidium iodide (PI) double staining. The expression of clusterin, XIAP and survivin on mRNA and protein levels was investigated by semi-quantitative RT-PCR and Western blotting, respectively. Results: Our data showed that 24 h treatment of LNCaP cells with 0.5 and 3.0 µM CPT resulted in higher number of apoptotic cells, than that in PC-3 cells. Western blot analysis revealed that the clusterin level in PC-3 cells was 5-fold higher than that in LNCaP cells. In contrast, XIAP expression level in PC-3 cells was lower than that in LNCaP cells, and survivin levels were similar in these two cell lines. Treatment with 0.5 and 3.0 µM CPT resulted in the reduced survivin and XIAP expression in both cell lines, while clusterin expression remained unchanged in LNCaP cells, but was increased in PC-3 cells. Conclusion: The results suggest that clusterin may take a greater part in CPT-resistance than survivin and XIAP in PC-3 cells.
format Article
author Mizutani, K.
Matsumoto, K.
Hasegawa, N.
Deguchi, T.
Nozawa, Y.
author_facet Mizutani, K.
Matsumoto, K.
Hasegawa, N.
Deguchi, T.
Nozawa, Y.
author_sort Mizutani, K.
title Expression of clusterin, XIAP and survivin, and their changes by camptothecin (CPT) treatment in CPT resistant PC-3 and CPT-sensitive LNCaP cells
title_short Expression of clusterin, XIAP and survivin, and their changes by camptothecin (CPT) treatment in CPT resistant PC-3 and CPT-sensitive LNCaP cells
title_full Expression of clusterin, XIAP and survivin, and their changes by camptothecin (CPT) treatment in CPT resistant PC-3 and CPT-sensitive LNCaP cells
title_fullStr Expression of clusterin, XIAP and survivin, and their changes by camptothecin (CPT) treatment in CPT resistant PC-3 and CPT-sensitive LNCaP cells
title_full_unstemmed Expression of clusterin, XIAP and survivin, and their changes by camptothecin (CPT) treatment in CPT resistant PC-3 and CPT-sensitive LNCaP cells
title_sort expression of clusterin, xiap and survivin, and their changes by camptothecin (cpt) treatment in cpt resistant pc-3 and cpt-sensitive lncap cells
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2006
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/137572
citation_txt Expression of clusterin, XIAP and survivin, and their changes by camptothecin (CPT) treatment in CPT resistant PC-3 and CPT-sensitive LNCaP cells / K. Mizutani, K. Matsumoto, N. Hasegawa, T. Deguchi, Y. Nozawa // Experimental Oncology. — 2006. — Т. 28, № 3. — С. 209-215. — Бібліогр.: 26 назв. — англ.
series Experimental Oncology
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AT deguchit expressionofclusterinxiapandsurvivinandtheirchangesbycamptothecincpttreatmentincptresistantpc3andcptsensitivelncapcells
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first_indexed 2023-10-18T21:14:32Z
last_indexed 2023-10-18T21:14:32Z
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