Involvement of human beta-defensin-2 in intracellular signaling: in vitro study

• Дата: 2006
• Автори: Lytvyn, D., Zhuravel, E., Markeeva, N.V., Soldatkina, M., Dudchenko, T., Pogrebnoy, P.V.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2006
• Назва видання: Experimental Oncology
• Теми: Original contributions
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/137576
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Involvement of human beta-defensin-2 in intracellular signaling: in vitro study / D. Lytvyn, E. Zhuravel, N.V. Markeeva, M. Soldatkina, T. Dudchenko, P.V. Pogrebnoy // Experimental Oncology. — 2006. — Т. 28, № 3. — С. 198-202. — Бібліогр.: 25 назв. — англ.

Aim: To analyze involvement of human beta-defensin-2 (hBD-2) in intracellular signaling in vitro. Materials and Methods: A431cells were cultured in the presence of 1 µg/ml of recombinant hBD-2 and/or 10 ng/ml EGF. For evaluation of expression of mRNAs for p70S6 kinase, isoforms alpha and beta, RT-PCR analysis was applied. Expression and activity of p70S6K, phosphorylation of PDK1, ERK, JNK, p38 kinases and EGF receptor (EGFR) was evaluated using Western blot analysis. Results: 30 min incubation of A431 cells with 1 µg/ml of hBD-2 didn’t influence autophosphorylation level of EGFR, but resulted in activation of p70S6K, 12 h treatment – in prominently increased level of mRNA for alpha and beta-isoforms of p70S6 kinase, whilst 24 h treatment – in elevation of p70S6K synthesis on protein level. Up-stream kinase phosphorylating p70S6K, PDK1, is also phosporylated upon influence of exogenous hBD-2 in vitro. Conclusion: Our data point on the involvement of PDK1-p70S6K pathway in mediation of action of hBD-2 in A431 cells.