Involvement of human beta-defensin-2 in intracellular signaling: in vitro study
Aim: To analyze involvement of human beta-defensin-2 (hBD-2) in intracellular signaling in vitro. Materials and Methods: A431cells were cultured in the presence of 1 µg/ml of recombinant hBD-2 and/or 10 ng/ml EGF. For evaluation of expression of mRNAs for p70S6 kinase, isoforms alpha and beta, RT-PC...
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| Дата: | 2006 |
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| Автори: | , , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2006
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/137576 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Involvement of human beta-defensin-2 in intracellular signaling: in vitro study / D. Lytvyn, E. Zhuravel, N.V. Markeeva, M. Soldatkina, T. Dudchenko, P.V. Pogrebnoy // Experimental Oncology. — 2006. — Т. 28, № 3. — С. 198-202. — Бібліогр.: 25 назв. — англ. |
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irk-123456789-1375762018-06-18T03:06:27Z Involvement of human beta-defensin-2 in intracellular signaling: in vitro study Lytvyn, D. Zhuravel, E. Markeeva, N.V. Soldatkina, M. Dudchenko, T. Pogrebnoy, P.V. Original contributions Aim: To analyze involvement of human beta-defensin-2 (hBD-2) in intracellular signaling in vitro. Materials and Methods: A431cells were cultured in the presence of 1 µg/ml of recombinant hBD-2 and/or 10 ng/ml EGF. For evaluation of expression of mRNAs for p70S6 kinase, isoforms alpha and beta, RT-PCR analysis was applied. Expression and activity of p70S6K, phosphorylation of PDK1, ERK, JNK, p38 kinases and EGF receptor (EGFR) was evaluated using Western blot analysis. Results: 30 min incubation of A431 cells with 1 µg/ml of hBD-2 didn’t influence autophosphorylation level of EGFR, but resulted in activation of p70S6K, 12 h treatment – in prominently increased level of mRNA for alpha and beta-isoforms of p70S6 kinase, whilst 24 h treatment – in elevation of p70S6K synthesis on protein level. Up-stream kinase phosphorylating p70S6K, PDK1, is also phosporylated upon influence of exogenous hBD-2 in vitro. Conclusion: Our data point on the involvement of PDK1-p70S6K pathway in mediation of action of hBD-2 in A431 cells. Цель: проанализировать участие бета-дефенсина-2 человека (hBD-2) в механизмах передачи внутриклеточных сигналов в модели in vitro. Материалы и методы: клетки линии A431 культивировали в присутствии 1 µг/мл рекомбинантного hBD-2 и/или 10 нг/мл ЭФР. Экспрессию мРНК альфа- и бета-изоформ p70S6 киназы оценивали методом полуколичественного ОТ-ПЦР анализа. Экспрессию и активность p70S6K, фосфорилирование PDK1, ERK, JNK, p38 киназ и рецептора ЭФР (ЭФРР) исследовали методом Вестерн-блот анализа. Результаты: 30 мин инкубация клеток A431 с 1 µг/мл hBD-2 не оказывала влияния на уровень аутофосфорилирования ЭФРР, но препятствовала образованию димеров рецептора в присутствии ЭФР. В то же время 30 мин обработка клеток hBD-2 приводила к активации p70S6K, 12 ч — к значительному повышению уровня мРНК альфа- и бета-изоформ p70S6 киназы, а 24 ч — к повышению синтеза p70S6K на уровне белка. PDK1-киназа, фосфорилирующая p70S6K, также подвергалась фосфорилированию в присутствии экзогенного hBD-2 in vitro. Выводы: данные свидетельствуют об участии каскада PDK1-p70S6K в опосредовании действия hBD-2 в клетках A431. 2006 Article Involvement of human beta-defensin-2 in intracellular signaling: in vitro study / D. Lytvyn, E. Zhuravel, N.V. Markeeva, M. Soldatkina, T. Dudchenko, P.V. Pogrebnoy // Experimental Oncology. — 2006. — Т. 28, № 3. — С. 198-202. — Бібліогр.: 25 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/137576 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Lytvyn, D. Zhuravel, E. Markeeva, N.V. Soldatkina, M. Dudchenko, T. Pogrebnoy, P.V. Involvement of human beta-defensin-2 in intracellular signaling: in vitro study Experimental Oncology |
| description |
Aim: To analyze involvement of human beta-defensin-2 (hBD-2) in intracellular signaling in vitro. Materials and Methods: A431cells were cultured in the presence of 1 µg/ml of recombinant hBD-2 and/or 10 ng/ml EGF. For evaluation of expression of mRNAs for p70S6 kinase, isoforms alpha and beta, RT-PCR analysis was applied. Expression and activity of p70S6K, phosphorylation of PDK1, ERK, JNK, p38 kinases and EGF receptor (EGFR) was evaluated using Western blot analysis. Results: 30 min incubation of A431 cells with 1 µg/ml of hBD-2 didn’t influence autophosphorylation level of EGFR, but resulted in activation of p70S6K, 12 h treatment – in prominently increased level of mRNA for alpha and beta-isoforms of p70S6 kinase, whilst 24 h treatment – in elevation of p70S6K synthesis on protein level. Up-stream kinase phosphorylating p70S6K, PDK1, is also phosporylated upon influence of exogenous hBD-2 in vitro. Conclusion: Our data point on the involvement of PDK1-p70S6K pathway in mediation of action of hBD-2 in A431 cells. |
| format |
Article |
| author |
Lytvyn, D. Zhuravel, E. Markeeva, N.V. Soldatkina, M. Dudchenko, T. Pogrebnoy, P.V. |
| author_facet |
Lytvyn, D. Zhuravel, E. Markeeva, N.V. Soldatkina, M. Dudchenko, T. Pogrebnoy, P.V. |
| author_sort |
Lytvyn, D. |
| title |
Involvement of human beta-defensin-2 in intracellular signaling: in vitro study |
| title_short |
Involvement of human beta-defensin-2 in intracellular signaling: in vitro study |
| title_full |
Involvement of human beta-defensin-2 in intracellular signaling: in vitro study |
| title_fullStr |
Involvement of human beta-defensin-2 in intracellular signaling: in vitro study |
| title_full_unstemmed |
Involvement of human beta-defensin-2 in intracellular signaling: in vitro study |
| title_sort |
involvement of human beta-defensin-2 in intracellular signaling: in vitro study |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2006 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/137576 |
| citation_txt |
Involvement of human beta-defensin-2 in intracellular signaling: in vitro study / D. Lytvyn,
E. Zhuravel, N.V. Markeeva, M. Soldatkina, T. Dudchenko, P.V. Pogrebnoy // Experimental Oncology. — 2006. — Т. 28, № 3. — С. 198-202. — Бібліогр.: 25 назв. — англ. |
| series |
Experimental Oncology |
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2023-10-18T21:14:32Z |
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