Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
In acute myeloid leukemia (AML) the functional abnormalities of osteopontin (OPN), NF-kB, PI3K/AKT/mTOR/PTEN pathway or β-catenin have been considered. Aim: To analyze the response of U937 cells to parthenolide (PTL) through the involvement of expression of OPN protein, RelB, AKT1, mTOR, PTEN and β-...
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| Дата: | 2017 |
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| Автори: | , , , , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2017
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/137611 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Parthenolide reduces gene transcription of prosurvival mediators in U937 cells / S. Mohammadi, M. Zahedpanah, M. Nikbakht, M. Shaiegan, S. Hamidollah Ghaffari, M. Nikugoftar, B. Rahmani, D. Hamedi Asl // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 30-35. — Бібліогр.: 55 назв. — англ. |
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irk-123456789-1376112018-06-18T03:10:17Z Parthenolide reduces gene transcription of prosurvival mediators in U937 cells Mohammadi, S. Zahedpanah, M. Nikbakht, M. Shaiegan, M. Hamidollah Ghaffari, S. Nikugoftar, M. Rahmani, B. Hamedi Asl, D. Original contributions In acute myeloid leukemia (AML) the functional abnormalities of osteopontin (OPN), NF-kB, PI3K/AKT/mTOR/PTEN pathway or β-catenin have been considered. Aim: To analyze the response of U937 cells to parthenolide (PTL) through the involvement of expression of OPN protein, RelB, AKT1, mTOR, PTEN and β-catenin genes. Materials and Methods: The U937 cells were treated with PTL at concentrations of 4 μM (IC25) or 6 μM (IC50) and with OPN siRNA for MTT assay and colony forming assay. Western blot analysis using antibodies against OPN was performed with lysates of PTL-treated cells. Quantitative real-time polymerase chain reaction was performed using primers for OPN siRNA, RelB, AKT1, mTOR, PTEN and β-catenin. Results: PTL reduces OPN protein level and down-regulates RelB mRNA in U937 cell line. Suppression of OPN with siRNA increases the cytotoxic effects of PTL. Also, mRNA expression of AKT1, mTOR, PTEN, and β-catenin decreases with PTL or OPN siRNA. Conclusion: Sensitivity of U937 cells to PTL can be associated with the reduction in expression of prosurvival mediators. 2017 Article Parthenolide reduces gene transcription of prosurvival mediators in U937 cells / S. Mohammadi, M. Zahedpanah, M. Nikbakht, M. Shaiegan, S. Hamidollah Ghaffari, M. Nikugoftar, B. Rahmani, D. Hamedi Asl // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 30-35. — Бібліогр.: 55 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/137611 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Mohammadi, S. Zahedpanah, M. Nikbakht, M. Shaiegan, M. Hamidollah Ghaffari, S. Nikugoftar, M. Rahmani, B. Hamedi Asl, D. Parthenolide reduces gene transcription of prosurvival mediators in U937 cells Experimental Oncology |
| description |
In acute myeloid leukemia (AML) the functional abnormalities of osteopontin (OPN), NF-kB, PI3K/AKT/mTOR/PTEN pathway or β-catenin have been considered. Aim: To analyze the response of U937 cells to parthenolide (PTL) through the involvement of expression of OPN protein, RelB, AKT1, mTOR, PTEN and β-catenin genes. Materials and Methods: The U937 cells were treated with PTL at concentrations of 4 μM (IC25) or 6 μM (IC50) and with OPN siRNA for MTT assay and colony forming assay. Western blot analysis using antibodies against OPN was performed with lysates of PTL-treated cells. Quantitative real-time polymerase chain reaction was performed using primers for OPN siRNA, RelB, AKT1, mTOR, PTEN and β-catenin. Results: PTL reduces OPN protein level and down-regulates RelB mRNA in U937 cell line. Suppression of OPN with siRNA increases the cytotoxic effects of PTL. Also, mRNA expression of AKT1, mTOR, PTEN, and β-catenin decreases with PTL or OPN siRNA. Conclusion: Sensitivity of U937 cells to PTL can be associated with the reduction in expression of prosurvival mediators. |
| format |
Article |
| author |
Mohammadi, S. Zahedpanah, M. Nikbakht, M. Shaiegan, M. Hamidollah Ghaffari, S. Nikugoftar, M. Rahmani, B. Hamedi Asl, D. |
| author_facet |
Mohammadi, S. Zahedpanah, M. Nikbakht, M. Shaiegan, M. Hamidollah Ghaffari, S. Nikugoftar, M. Rahmani, B. Hamedi Asl, D. |
| author_sort |
Mohammadi, S. |
| title |
Parthenolide reduces gene transcription of prosurvival mediators in U937 cells |
| title_short |
Parthenolide reduces gene transcription of prosurvival mediators in U937 cells |
| title_full |
Parthenolide reduces gene transcription of prosurvival mediators in U937 cells |
| title_fullStr |
Parthenolide reduces gene transcription of prosurvival mediators in U937 cells |
| title_full_unstemmed |
Parthenolide reduces gene transcription of prosurvival mediators in U937 cells |
| title_sort |
parthenolide reduces gene transcription of prosurvival mediators in u937 cells |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2017 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/137611 |
| citation_txt |
Parthenolide reduces gene transcription of prosurvival mediators in U937 cells / S. Mohammadi, M. Zahedpanah, M. Nikbakht, M. Shaiegan, S. Hamidollah Ghaffari, M. Nikugoftar, B. Rahmani, D. Hamedi Asl // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 30-35. — Бібліогр.: 55 назв. — англ. |
| series |
Experimental Oncology |
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2023-10-18T21:16:09Z |
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