Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa
Aim: To analyze the glycoprotein binding sites of the gastric mucosa and its secreted mucus using lectin histochemistry in patients with chronic non-atrophic gastritis (CNAG) associated or not-associated with Helicobacter pylori infection with or without dysplasia. Materials and Methods: In order to...
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| Дата: | 2017 |
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| Автори: | , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2017
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/137631 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa / S. Vernygorodskyi, V. Shkolnikov, D. Suhan // Experimental Oncology. — 2017 — Т. 39, № 2. — С. 138–140. — Бібліогр.: 7 назв. — англ. |
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irk-123456789-1376312018-06-18T03:10:35Z Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa Vernygorodskyi, S. Shkolnikov, V. Suhan, D. Original contributions Aim: To analyze the glycoprotein binding sites of the gastric mucosa and its secreted mucus using lectin histochemistry in patients with chronic non-atrophic gastritis (CNAG) associated or not-associated with Helicobacter pylori infection with or without dysplasia. Materials and Methods: In order to identify the areas with glycoconjugates expression in gastric mucosa, 6 lectins (Canavalia ensiformis agglutinin — Con A, Sambucus nigra agglutinin — SNA, wheat germ agglutinin — WGA, soybean agglutinin — SBA, Helix pomatia agglutinin — HPA, peanut agglutinin — PNA) were used. Carbohydrate determinants were visualized according to the lectin-peroxidase-diaminobenzidine staining protocol. Biopsy material was obtained and processed by conventional histological methods. The samples from 84 patients (54 with CNAG) with low (n = 34) and high grade (n = 20) dysplasia, 38 patients were H. pylori-infected and 26 patients — H. pylori-noninfected) were used. The comparison group included 30 persons with CNAG without dysplasia (16 patients H. pylori-infected and 14 — noninfected). Results: In comparison to normal gastric mucosa, a low affinity of Con A was shown in 80% of patients with non-infected CNAG and 90% of H. pylori associated CNAG. In 70% of H. pylori-infected patients with CNAG and low grade dysplasia there was an increase of SNA expression compared with noninfected patients (p < 0.05). Regarding SBA labeling no differences were detected in the studied groups (p < 0.05). In H. pylori infected patients with CNAG and low grade dysplasia, WGA, HPA and PNA showed a strong reactivity with the gastric mucosa cells in 80; 75%, and 60% of patients, respectively. Conclusion: We suggest that a set of lectins in reaction with gastric epithelial and glandular cells can be used as a tool to obtain information about the dysplastic changes of the gastric mucosa and may offer new insight into gastric carcinogenesis and precancerous lesions treatment. 2017 Article Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa / S. Vernygorodskyi, V. Shkolnikov, D. Suhan // Experimental Oncology. — 2017 — Т. 39, № 2. — С. 138–140. — Бібліогр.: 7 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/137631 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Vernygorodskyi, S. Shkolnikov, V. Suhan, D. Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa Experimental Oncology |
| description |
Aim: To analyze the glycoprotein binding sites of the gastric mucosa and its secreted mucus using lectin histochemistry in patients with chronic non-atrophic gastritis (CNAG) associated or not-associated with Helicobacter pylori infection with or without dysplasia. Materials and Methods: In order to identify the areas with glycoconjugates expression in gastric mucosa, 6 lectins (Canavalia ensiformis agglutinin — Con A, Sambucus nigra agglutinin — SNA, wheat germ agglutinin — WGA, soybean agglutinin — SBA, Helix pomatia agglutinin — HPA, peanut agglutinin — PNA) were used. Carbohydrate determinants were visualized according to the lectin-peroxidase-diaminobenzidine staining protocol. Biopsy material was obtained and processed by conventional histological methods. The samples from 84 patients (54 with CNAG) with low (n = 34) and high grade (n = 20) dysplasia, 38 patients were H. pylori-infected and 26 patients — H. pylori-noninfected) were used. The comparison group included 30 persons with CNAG without dysplasia (16 patients H. pylori-infected and 14 — noninfected). Results: In comparison to normal gastric mucosa, a low affinity of Con A was shown in 80% of patients with non-infected CNAG and 90% of H. pylori associated CNAG. In 70% of H. pylori-infected patients with CNAG and low grade dysplasia there was an increase of SNA expression compared with noninfected patients (p < 0.05). Regarding SBA labeling no differences were detected in the studied groups (p < 0.05). In H. pylori infected patients with CNAG and low grade dysplasia, WGA, HPA and PNA showed a strong reactivity with the gastric mucosa cells in 80; 75%, and 60% of patients, respectively. Conclusion: We suggest that a set of lectins in reaction with gastric epithelial and glandular cells can be used as a tool to obtain information about the dysplastic changes of the gastric mucosa and may offer new insight into gastric carcinogenesis and precancerous lesions treatment. |
| format |
Article |
| author |
Vernygorodskyi, S. Shkolnikov, V. Suhan, D. |
| author_facet |
Vernygorodskyi, S. Shkolnikov, V. Suhan, D. |
| author_sort |
Vernygorodskyi, S. |
| title |
Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa |
| title_short |
Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa |
| title_full |
Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa |
| title_fullStr |
Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa |
| title_full_unstemmed |
Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa |
| title_sort |
lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2017 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/137631 |
| citation_txt |
Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa / S. Vernygorodskyi, V. Shkolnikov, D. Suhan // Experimental Oncology. — 2017 — Т. 39, № 2. — С. 138–140. — Бібліогр.: 7 назв. — англ. |
| series |
Experimental Oncology |
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| first_indexed |
2023-10-18T21:16:24Z |
| last_indexed |
2023-10-18T21:16:24Z |
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