High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels

High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels

Aim: To study the relationship between the level of generation of reactive oxygen species (ROS) and nitric oxide (NO) and activity of matrix metalloproteinases (MMPs) MMP-2 and MMP-9 in the vessels isolated from rectal tumors and Arteria rectalis superior. Methods: EPR at the room temperature and 77...

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Бібліографічні деталі
Дата:2006
Автори: Burlaka, A.P., Sidorik, E.P., Ganusevich, I.I., Lestchenko, Yu.M., Burlaka, A.А., Osinsky, S.P.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2006
Назва видання:Experimental Oncology
Теми:
Short communications
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/137932
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels / A.P. Burlaka, E.P. Sidorik, I.I. Ganusevich, Yu.M. Lestchenko, A.A. Burlaka, S.P. Osinsky // Experimental Oncology. — 2006. — Т. 28, № 4. — С. 323-325. — Бібліогр.: 13 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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spelling irk-123456789-1379322018-06-18T03:10:57Z High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels Burlaka, A.P. Sidorik, E.P. Ganusevich, I.I. Lestchenko, Yu.M. Burlaka, A.А. Osinsky, S.P. Short communications Aim: To study the relationship between the level of generation of reactive oxygen species (ROS) and nitric oxide (NO) and activity of matrix metalloproteinases (MMPs) MMP-2 and MMP-9 in the vessels isolated from rectal tumors and Arteria rectalis superior. Methods: EPR at the room temperature and 77 °K, Spin Traps technology and zymography in polyacrylamide gels were applied. Results: In the vessels isolated from rectal tumors and Arteria rectalis superior high levels of ROS, NO· and formation of complexes of NO· with FeS-proteins at the sites of electron-transporting chain of mitochondria have been detected. High activities of MMP-2 and MMP-9 in vascular wall were also observed. The direct positive correlation between the rate of NO· generation and formation of complexes of NO·· with FeS-proteins as well as between ROS and NO formation and MMPs activities have been revealed. Conclusion: Altered oxidative equilibrium in mitochondria of cells in vascular wall promotes formation of cell hypoxia and its autocatalytic potentiation accompanied with activation of MMPs. Цель: изучить взаимоотношение между уровнями образования активных форм кислорода (АФК) и оксида азота (ОА) и активности матриксных металлопротеиназ (MMPs) MMP-2 и MMP-9 в сосудах, изолированных из опухолевой ткани прямой кишки, и в Arteria rectalis superior. Методы: ЭПР при комнатной температуре и при 77 °K, технология спиновых ловушек, зимофрафия в полиакриламидном геле. Результаты: в сосудах, изолированных из опухолевой ткани прямой кишки, и в Arteria rectalis superior обнаружены высокие уровни образования АФК, · и комплексов · с FeS-белками в участках цепи митохондрий. Определена также высокая активность MMP-2 и MMP-9 в стенке сосудов, находящихся в опухолевом узле. Обнаружена прямая положительная корреляция между величиной образования · и образованием комплексов · с белками, а также между образованием АФК и NO и активность MMPs. Выводы: измененное окислительное равновесие в митохондриях клеток стенки сосудов, находящихся в опухолевой ткани, усиливает формирование в них клеточной гипоксии, ее аутокаталитическое потенцирование, что сопровождается активацией в этих клетках MMPs 2006 Article High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels / A.P. Burlaka, E.P. Sidorik, I.I. Ganusevich, Yu.M. Lestchenko, A.A. Burlaka, S.P. Osinsky // Experimental Oncology. — 2006. — Т. 28, № 4. — С. 323-325. — Бібліогр.: 13 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/137932 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Short communications
Short communications
spellingShingle Short communications
Short communications
Burlaka, A.P.
Sidorik, E.P.
Ganusevich, I.I.
Lestchenko, Yu.M.
Burlaka, A.А.
Osinsky, S.P.
High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels
Experimental Oncology
description Aim: To study the relationship between the level of generation of reactive oxygen species (ROS) and nitric oxide (NO) and activity of matrix metalloproteinases (MMPs) MMP-2 and MMP-9 in the vessels isolated from rectal tumors and Arteria rectalis superior. Methods: EPR at the room temperature and 77 °K, Spin Traps technology and zymography in polyacrylamide gels were applied. Results: In the vessels isolated from rectal tumors and Arteria rectalis superior high levels of ROS, NO· and formation of complexes of NO· with FeS-proteins at the sites of electron-transporting chain of mitochondria have been detected. High activities of MMP-2 and MMP-9 in vascular wall were also observed. The direct positive correlation between the rate of NO· generation and formation of complexes of NO·· with FeS-proteins as well as between ROS and NO formation and MMPs activities have been revealed. Conclusion: Altered oxidative equilibrium in mitochondria of cells in vascular wall promotes formation of cell hypoxia and its autocatalytic potentiation accompanied with activation of MMPs.
format Article
author Burlaka, A.P.
Sidorik, E.P.
Ganusevich, I.I.
Lestchenko, Yu.M.
Burlaka, A.А.
Osinsky, S.P.
author_facet Burlaka, A.P.
Sidorik, E.P.
Ganusevich, I.I.
Lestchenko, Yu.M.
Burlaka, A.А.
Osinsky, S.P.
author_sort Burlaka, A.P.
title High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels
title_short High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels
title_full High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels
title_fullStr High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels
title_full_unstemmed High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels
title_sort high formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2006
topic_facet Short communications
url http://dspace.nbuv.gov.ua/handle/123456789/137932
citation_txt High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels / A.P. Burlaka, E.P. Sidorik, I.I. Ganusevich, Yu.M. Lestchenko, A.A. Burlaka, S.P. Osinsky // Experimental Oncology. — 2006. — Т. 28, № 4. — С. 323-325. — Бібліогр.: 13 назв. — англ.
series Experimental Oncology
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first_indexed 2023-10-18T21:16:46Z
last_indexed 2023-10-18T21:16:46Z
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