The effect of racemic gossypol and AT-101 on angiogenic profile of OVCAR-3 cells: a preliminary molecular framework for gossypol enantiomers

The effect of racemic gossypol and AT-101 on angiogenic profile of OVCAR-3 cells: a preliminary molecular framework for gossypol enantiomers

To compare the effect of racemic gossypol with its (–)/(–) enantiomer (AT-101) on expression profiles of angiogenic molecules by mRNA levels in human ovarian cancer cell line OVCAR-3. Methods: Cell viability assay (2,3-bis (2-methoxy-4-nitro-5- sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium...

Повний опис

Збережено в:
Бібліографічні деталі
Дата:2009
Автори: Varol, U., Karaca, B., Tunali, D., Degirmenci, M., Cirak, Y., Purcu, D.U., Uzunoglu, S., Sezgin, C., Karabulut, B., Sanli, U.A., Uslu, R.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2009
Назва видання:Experimental Oncology
Теми:
Original contributions
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/138141
Теги: Додати тег
Немає тегів, Будьте першим, хто поставить тег для цього запису!
Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:The effect of racemic gossypol and AT-101 on angiogenic profile of OVCAR-3 cells: a preliminary molecular framework for gossypol enantiomers / U. Varol, B. Karaca, D. Tunali, M. Degirmenci, Y. Cirak, D.U. Purcu, S. Uzunolgu, C. Sezgin, B. Karabulut, U.A. Sanli, R. Uslu // Experimental Oncology. — 2009. — Т. 31, № 4. — С. 220-225. — Бібліогр.: 34 назв. — англ.

Репозитарії

Digital Library of Periodicals of National Academy of Sciences of Ukraine
id irk-123456789-138141
record_format dspace
spelling irk-123456789-1381412018-06-19T03:03:34Z The effect of racemic gossypol and AT-101 on angiogenic profile of OVCAR-3 cells: a preliminary molecular framework for gossypol enantiomers Varol, U. Karaca, B. Tunali, D. Degirmenci, M. Cirak, Y. Purcu, D.U. Uzunoglu, S. Sezgin, C. Karabulut, B. Sanli, U.A. Uslu, R. Original contributions To compare the effect of racemic gossypol with its (–)/(–) enantiomer (AT-101) on expression profiles of angiogenic molecules by mRNA levels in human ovarian cancer cell line OVCAR-3. Methods: Cell viability assay (2,3-bis (2-methoxy-4-nitro-5- sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide) was used to detect cytotoxicity of gossypol enantiomers. DNA fragmentation by an enzyme-linked immunosorbent (ELISA) assay was used to evaluate the rate of apoptosis. The mRNA expression levels of angiogenic molecules were investigated by Human Angiogenesis RT2 ProfilerTM PCR Array (SuperArray, Frederick, MD). Results: Both racemic form and AT-101 resulted in a significant cytotoxicity and induced apoptosis. This effect was observed in a dose- and time dependent manner. However, AT-101 was much more potent. In addition, the treatment of 10 μM of racemic gossypol alone and 3 μM of AT-101 alone resulted in significant down-regulation (≥ 3 fold) in mRNA levels of some pivotal angiogenic molecules in OVCAR-3, but altered gene profiles were different by the treatment of each enantiomer. Conclusion: The efficacy of two gossypol enantiomers in OVCAR-3 cells showed distinction. AT-101 was much more potent than racemic gossypol, not only by means of cell death and apoptosis, but also by modulation of angiogenic molecules released from OVCAR-3 cells. Further studies with endothelial cells should be done to verify the anti-angiogenic effect of gossypol enantiomers in cancer treatment. 2009 Article The effect of racemic gossypol and AT-101 on angiogenic profile of OVCAR-3 cells: a preliminary molecular framework for gossypol enantiomers / U. Varol, B. Karaca, D. Tunali, M. Degirmenci, Y. Cirak, D.U. Purcu, S. Uzunolgu, C. Sezgin, B. Karabulut, U.A. Sanli, R. Uslu // Experimental Oncology. — 2009. — Т. 31, № 4. — С. 220-225. — Бібліогр.: 34 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138141 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Varol, U.
Karaca, B.
Tunali, D.
Degirmenci, M.
Cirak, Y.
Purcu, D.U.
Uzunoglu, S.
Sezgin, C.
Karabulut, B.
Sanli, U.A.
Uslu, R.
The effect of racemic gossypol and AT-101 on angiogenic profile of OVCAR-3 cells: a preliminary molecular framework for gossypol enantiomers
Experimental Oncology
description To compare the effect of racemic gossypol with its (–)/(–) enantiomer (AT-101) on expression profiles of angiogenic molecules by mRNA levels in human ovarian cancer cell line OVCAR-3. Methods: Cell viability assay (2,3-bis (2-methoxy-4-nitro-5- sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide) was used to detect cytotoxicity of gossypol enantiomers. DNA fragmentation by an enzyme-linked immunosorbent (ELISA) assay was used to evaluate the rate of apoptosis. The mRNA expression levels of angiogenic molecules were investigated by Human Angiogenesis RT2 ProfilerTM PCR Array (SuperArray, Frederick, MD). Results: Both racemic form and AT-101 resulted in a significant cytotoxicity and induced apoptosis. This effect was observed in a dose- and time dependent manner. However, AT-101 was much more potent. In addition, the treatment of 10 μM of racemic gossypol alone and 3 μM of AT-101 alone resulted in significant down-regulation (≥ 3 fold) in mRNA levels of some pivotal angiogenic molecules in OVCAR-3, but altered gene profiles were different by the treatment of each enantiomer. Conclusion: The efficacy of two gossypol enantiomers in OVCAR-3 cells showed distinction. AT-101 was much more potent than racemic gossypol, not only by means of cell death and apoptosis, but also by modulation of angiogenic molecules released from OVCAR-3 cells. Further studies with endothelial cells should be done to verify the anti-angiogenic effect of gossypol enantiomers in cancer treatment.
format Article
author Varol, U.
Karaca, B.
Tunali, D.
Degirmenci, M.
Cirak, Y.
Purcu, D.U.
Uzunoglu, S.
Sezgin, C.
Karabulut, B.
Sanli, U.A.
Uslu, R.
author_facet Varol, U.
Karaca, B.
Tunali, D.
Degirmenci, M.
Cirak, Y.
Purcu, D.U.
Uzunoglu, S.
Sezgin, C.
Karabulut, B.
Sanli, U.A.
Uslu, R.
author_sort Varol, U.
title The effect of racemic gossypol and AT-101 on angiogenic profile of OVCAR-3 cells: a preliminary molecular framework for gossypol enantiomers
title_short The effect of racemic gossypol and AT-101 on angiogenic profile of OVCAR-3 cells: a preliminary molecular framework for gossypol enantiomers
title_full The effect of racemic gossypol and AT-101 on angiogenic profile of OVCAR-3 cells: a preliminary molecular framework for gossypol enantiomers
title_fullStr The effect of racemic gossypol and AT-101 on angiogenic profile of OVCAR-3 cells: a preliminary molecular framework for gossypol enantiomers
title_full_unstemmed The effect of racemic gossypol and AT-101 on angiogenic profile of OVCAR-3 cells: a preliminary molecular framework for gossypol enantiomers
title_sort effect of racemic gossypol and at-101 on angiogenic profile of ovcar-3 cells: a preliminary molecular framework for gossypol enantiomers
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2009
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/138141
citation_txt The effect of racemic gossypol and AT-101 on angiogenic profile of OVCAR-3 cells: a preliminary molecular framework for gossypol enantiomers / U. Varol, B. Karaca, D. Tunali, M. Degirmenci, Y. Cirak, D.U. Purcu, S. Uzunolgu, C. Sezgin, B. Karabulut, U.A. Sanli, R. Uslu // Experimental Oncology. — 2009. — Т. 31, № 4. — С. 220-225. — Бібліогр.: 34 назв. — англ.
series Experimental Oncology
work_keys_str_mv AT varolu theeffectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT karacab theeffectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT tunalid theeffectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT degirmencim theeffectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT ciraky theeffectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT purcudu theeffectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT uzunoglus theeffectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT sezginc theeffectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT karabulutb theeffectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT sanliua theeffectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT uslur theeffectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT varolu effectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT karacab effectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT tunalid effectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT degirmencim effectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT ciraky effectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT purcudu effectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT uzunoglus effectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT sezginc effectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT karabulutb effectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT sanliua effectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
AT uslur effectofracemicgossypolandat101onangiogenicprofileofovcar3cellsapreliminarymolecularframeworkforgossypolenantiomers
first_indexed 2023-10-18T21:17:32Z
last_indexed 2023-10-18T21:17:32Z
_version_ 1796152424045477888

Схожі ресурси