Serum factors that suppress cytotoxic effect of methotrexate
To study the phenomenon that human erythroid leukemia K-562 cells are more sensitive to cytotoxic effect of antimetabolites when cultured in a serum-free medium than in a conventional medium containing fetal calf serum (FCS). Methods: Cytotoxic effects of methotrexate, azaserine and 5-fluorouracil w...
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| Дата: | 2009 |
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| Автори: | , , , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2009
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| Назва видання: | Experimental Oncology |
| Теми: | |
| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/138204 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Serum factors that suppress cytotoxic effect of methotrexate / T. Kojima, Y. Hashimoto, Y. Inamura, T. Koide, H. Nagata, N. Ito, K. Suzumura // Experimental Oncology. — 2009. — Т. 31, № 4. — С. 209-213. — Бібліогр.: 8 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| id |
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irk-123456789-1382042018-06-19T03:03:56Z Serum factors that suppress cytotoxic effect of methotrexate Kojima, T. Hashimoto, Y. Inamura, Y. Koide, T. Nagata, H. Ito, N. Suzumura, K. Original contributions To study the phenomenon that human erythroid leukemia K-562 cells are more sensitive to cytotoxic effect of antimetabolites when cultured in a serum-free medium than in a conventional medium containing fetal calf serum (FCS). Methods: Cytotoxic effects of methotrexate, azaserine and 5-fluorouracil were estimated by accessing the lactate dehydrogenase (LDH) activity of viable tumor cells. Proteins of FCS were separated using two-dimensional electrophoresis followed by mass spectrometry analysis. Results: Addition of 10% FCS attenuated anti-tumor activity of methotrexate and azaserine against K-562 cells compared with serum-free medium. Such an activity of FCS was different for each serum lot. Comparison of the proteins in active serum lot with those in not active one using two-dimensional electrophoresis showed that in the active serum there were proteins 150 kDa, which were absent in the not active serum lot. Mass spectrometry indicated that all those proteins had the amino acid sequence of albumin. Sera of one healthy volunteer and two patients with thyroid cancer also attenuated the activity of the agent. Conclusion: Several lots of FCS and human serum demonstrated the ability to attenuate the cytotoxic effect of methotrexate in vitro, possibly due to the formation of albumin dimers/MTX complexes. 2009 Article Serum factors that suppress cytotoxic effect of methotrexate / T. Kojima, Y. Hashimoto, Y. Inamura, T. Koide, H. Nagata, N. Ito, K. Suzumura // Experimental Oncology. — 2009. — Т. 31, № 4. — С. 209-213. — Бібліогр.: 8 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138204 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Kojima, T. Hashimoto, Y. Inamura, Y. Koide, T. Nagata, H. Ito, N. Suzumura, K. Serum factors that suppress cytotoxic effect of methotrexate Experimental Oncology |
| description |
To study the phenomenon that human erythroid leukemia K-562 cells are more sensitive to cytotoxic effect of antimetabolites when cultured in a serum-free medium than in a conventional medium containing fetal calf serum (FCS). Methods: Cytotoxic effects of methotrexate, azaserine and 5-fluorouracil were estimated by accessing the lactate dehydrogenase (LDH) activity of viable tumor cells. Proteins of FCS were separated using two-dimensional electrophoresis followed by mass spectrometry analysis. Results: Addition of 10% FCS attenuated anti-tumor activity of methotrexate and azaserine against K-562 cells compared with serum-free medium. Such an activity of FCS was different for each serum lot. Comparison of the proteins in active serum lot with those in not active one using two-dimensional electrophoresis showed that in the active serum there were proteins 150 kDa, which were absent in the not active serum lot. Mass spectrometry indicated that all those proteins had the amino acid sequence of albumin. Sera of one healthy volunteer and two patients with thyroid cancer also attenuated the activity of the agent. Conclusion: Several lots of FCS and human serum demonstrated the ability to attenuate the cytotoxic effect of methotrexate in vitro, possibly due to the formation of albumin dimers/MTX complexes. |
| format |
Article |
| author |
Kojima, T. Hashimoto, Y. Inamura, Y. Koide, T. Nagata, H. Ito, N. Suzumura, K. |
| author_facet |
Kojima, T. Hashimoto, Y. Inamura, Y. Koide, T. Nagata, H. Ito, N. Suzumura, K. |
| author_sort |
Kojima, T. |
| title |
Serum factors that suppress cytotoxic effect of methotrexate |
| title_short |
Serum factors that suppress cytotoxic effect of methotrexate |
| title_full |
Serum factors that suppress cytotoxic effect of methotrexate |
| title_fullStr |
Serum factors that suppress cytotoxic effect of methotrexate |
| title_full_unstemmed |
Serum factors that suppress cytotoxic effect of methotrexate |
| title_sort |
serum factors that suppress cytotoxic effect of methotrexate |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2009 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/138204 |
| citation_txt |
Serum factors that suppress cytotoxic effect of methotrexate / T. Kojima, Y. Hashimoto, Y. Inamura, T. Koide, H. Nagata, N. Ito, K. Suzumura // Experimental Oncology. — 2009. — Т. 31, № 4. — С. 209-213. — Бібліогр.: 8 назв. — англ. |
| series |
Experimental Oncology |
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