Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications

Aim: The purpose of this work was to obtain, multiply and characterize the adult neural crest-derived multipotent stem cells from human hair follicle for their further clinical use. Materials and Methods: Adult neural crest-derived multipotent stem cells were obtained from human hair follicle by exp...

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Дата:2017
Автори: Vasyliev, R.G., Rodnichenko, A.E., Gubar, O.S., Zlatska, A.V., Gordiienko, I.M., Novikova, S.N., Zubov, D.O.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2017
Назва видання:Experimental Oncology
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Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/138539
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications / R.G. Vasyliev, A.E. Rodnichenko, O.S. Gubar, A.V. Zlatska, I.M. Gordiienko, S.N. Novikova, D.O. Zubov // Experimental Oncology. — 2017 — Т. 39, № 3. — С. 171–180. — Бібліогр.: 29 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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spelling irk-123456789-1385392018-06-20T03:04:42Z Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications Vasyliev, R.G. Rodnichenko, A.E. Gubar, O.S. Zlatska, A.V. Gordiienko, I.M. Novikova, S.N. Zubov, D.O. Original contributions Aim: The purpose of this work was to obtain, multiply and characterize the adult neural crest-derived multipotent stem cells from human hair follicle for their further clinical use. Materials and Methods: Adult neural crest-derived multipotent stem cells were obtained from human hair follicle by explant method and were expanded at large-scale up to a clinically significant number. The resulted cell cultures were examined by flow cytometry and immunocytochemical analysis. Their clonogenic potential, ability to self-renewal and directed multilineage differentiation were also investigated. Results: Cell cultures were obtained from explants of adult human hair follicles. Resulted cells according to morphological, phenotypic and functional criteria satisfied the definition of neural crest-derived multipotent stem cells. They had the phenotype Sox2⁺Sox10⁺Nestin⁺CD73⁺CD90⁺CD105⁺CD140a⁺CD 140b⁺CD146⁺CD166⁺CD271⁺CD349⁺ CD34⁻CD45⁻CD56⁻HLA⁻DR⁻, showed high clonogenic potential, ability to self-renewal and directed differentiation into the main derivatives of the neural crest: neurons, Schwann cells, adipocytes and osteoblasts. Conclusion: The possibility of a large-scale expansion of adult neural crest-derived multipotent stem cells up to 40–200·106 cells from minimal number of hair follicles with retention of their phenotype and functional properties are the significant step towards their translation into the clinical practice. 2017 Article Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications / R.G. Vasyliev, A.E. Rodnichenko, O.S. Gubar, A.V. Zlatska, I.M. Gordiienko, S.N. Novikova, D.O. Zubov // Experimental Oncology. — 2017 — Т. 39, № 3. — С. 171–180. — Бібліогр.: 29 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138539 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Vasyliev, R.G.
Rodnichenko, A.E.
Gubar, O.S.
Zlatska, A.V.
Gordiienko, I.M.
Novikova, S.N.
Zubov, D.O.
Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications
Experimental Oncology
description Aim: The purpose of this work was to obtain, multiply and characterize the adult neural crest-derived multipotent stem cells from human hair follicle for their further clinical use. Materials and Methods: Adult neural crest-derived multipotent stem cells were obtained from human hair follicle by explant method and were expanded at large-scale up to a clinically significant number. The resulted cell cultures were examined by flow cytometry and immunocytochemical analysis. Their clonogenic potential, ability to self-renewal and directed multilineage differentiation were also investigated. Results: Cell cultures were obtained from explants of adult human hair follicles. Resulted cells according to morphological, phenotypic and functional criteria satisfied the definition of neural crest-derived multipotent stem cells. They had the phenotype Sox2⁺Sox10⁺Nestin⁺CD73⁺CD90⁺CD105⁺CD140a⁺CD 140b⁺CD146⁺CD166⁺CD271⁺CD349⁺ CD34⁻CD45⁻CD56⁻HLA⁻DR⁻, showed high clonogenic potential, ability to self-renewal and directed differentiation into the main derivatives of the neural crest: neurons, Schwann cells, adipocytes and osteoblasts. Conclusion: The possibility of a large-scale expansion of adult neural crest-derived multipotent stem cells up to 40–200·106 cells from minimal number of hair follicles with retention of their phenotype and functional properties are the significant step towards their translation into the clinical practice.
format Article
author Vasyliev, R.G.
Rodnichenko, A.E.
Gubar, O.S.
Zlatska, A.V.
Gordiienko, I.M.
Novikova, S.N.
Zubov, D.O.
author_facet Vasyliev, R.G.
Rodnichenko, A.E.
Gubar, O.S.
Zlatska, A.V.
Gordiienko, I.M.
Novikova, S.N.
Zubov, D.O.
author_sort Vasyliev, R.G.
title Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications
title_short Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications
title_full Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications
title_fullStr Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications
title_full_unstemmed Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications
title_sort large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2017
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/138539
citation_txt Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications / R.G. Vasyliev, A.E. Rodnichenko, O.S. Gubar, A.V. Zlatska, I.M. Gordiienko, S.N. Novikova, D.O. Zubov // Experimental Oncology. — 2017 — Т. 39, № 3. — С. 171–180. — Бібліогр.: 29 назв. — англ.
series Experimental Oncology
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first_indexed 2023-10-18T21:17:56Z
last_indexed 2023-10-18T21:17:56Z
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