DNA damage in tumor cells and peripheral blood lymphocytes of endometrial cancer patients assessed by the comet assay

DNA damage in tumor cells and peripheral blood lymphocytes of endometrial cancer patients assessed by the comet assay

To date, genome instability is considered to be a common feature not only of tumor cells, but also of non-malignant cells of cancer patients, including peripheral blood lymphocytes (PBLs). The issue of the association between genome instability in tumor cells and PBLs, as well as of its relationship...

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Дата:2017
Автори: Buchynska, L.G., Brieieva, O.V., Lurchenko, N.P., Protsenko, V.V., Nespryadko, S.V.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2017
Назва видання:Experimental Oncology
Теми:
Original contributions
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/138546
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:DNA damage in tumor cells and peripheral blood lymphocytes of endometrial cancer patients assessed by the comet assay / L.G. Buchynska, O.V. Brieieva, N.P. Iurchenko, V.V. Protsenko, S.V. Nespryadko // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 299–303. — Бібліогр.: 35 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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spelling irk-123456789-1385462018-06-20T03:04:27Z DNA damage in tumor cells and peripheral blood lymphocytes of endometrial cancer patients assessed by the comet assay Buchynska, L.G. Brieieva, O.V. Lurchenko, N.P. Protsenko, V.V. Nespryadko, S.V. Original contributions To date, genome instability is considered to be a common feature not only of tumor cells, but also of non-malignant cells of cancer patients, including peripheral blood lymphocytes (PBLs). The issue of the association between genome instability in tumor cells and PBLs, as well as of its relationship with tumor progression remains poorly understood. Aim: To evaluate the level DNA damage in tumor cells and PBLs of endometrial cancer (EC) patients with regard to clinical and morphological characteristics of the patients. Materials and Methods: DNA damage was assessed in 106 PBLs samples and 42 samples of tumor cell suspension from EC patients by comet assay. PBLs from 30 healthy women were used as control. The level of DNA damage was expressed as the percentage of DNA in the comet tails (% tail DNA). Results: It was revealed that the amount of DNA damage in PBLs of EC patients was 2.2 times higher in comparison with that of healthy donors (8.3 ± 0.7 and 3.7 ± 0.4% tail DNA, respectively) (p < 0.05). In this study, no association between the levels of DNA damage in endometrial tumor cells and PBLs was observed (r = 0.11; p > 0.05). The amounts of DNA damage both in tumor cells and PBLs were not related to the degree of tumor differentiation as well as the depth of myometrial invasion, but depended on the body mass index (BMI) of EC patients: high level of lesions was observed in patients with elevated BMI values. Furthermore, the level of DNA damage in tumor cells was associated to familial aggregation of cancer and was significantly higher in endometrial cells from patients with family history of cancer vs that from EC patients with sporadic tumors (32.3 ± 2.9 and 22.8 ± 1.8% tail DNA, respectively) (p < 0.05). It was also found that for women who had high level of DNA damage in PBLs, the risk of EC was greater (odds ratio value of 3.5) compared to those with low level of such lesions. Conclusion: Genome instability that appears as an increased level of DNA damage in tumor cells and PBLs of EC patients is associated with BMI and family history of cancer and can reflect a predisposition to cancer. 2017 Article DNA damage in tumor cells and peripheral blood lymphocytes of endometrial cancer patients assessed by the comet assay / L.G. Buchynska, O.V. Brieieva, N.P. Iurchenko, V.V. Protsenko, S.V. Nespryadko // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 299–303. — Бібліогр.: 35 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138546 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Buchynska, L.G.
Brieieva, O.V.
Lurchenko, N.P.
Protsenko, V.V.
Nespryadko, S.V.
DNA damage in tumor cells and peripheral blood lymphocytes of endometrial cancer patients assessed by the comet assay
Experimental Oncology
description To date, genome instability is considered to be a common feature not only of tumor cells, but also of non-malignant cells of cancer patients, including peripheral blood lymphocytes (PBLs). The issue of the association between genome instability in tumor cells and PBLs, as well as of its relationship with tumor progression remains poorly understood. Aim: To evaluate the level DNA damage in tumor cells and PBLs of endometrial cancer (EC) patients with regard to clinical and morphological characteristics of the patients. Materials and Methods: DNA damage was assessed in 106 PBLs samples and 42 samples of tumor cell suspension from EC patients by comet assay. PBLs from 30 healthy women were used as control. The level of DNA damage was expressed as the percentage of DNA in the comet tails (% tail DNA). Results: It was revealed that the amount of DNA damage in PBLs of EC patients was 2.2 times higher in comparison with that of healthy donors (8.3 ± 0.7 and 3.7 ± 0.4% tail DNA, respectively) (p < 0.05). In this study, no association between the levels of DNA damage in endometrial tumor cells and PBLs was observed (r = 0.11; p > 0.05). The amounts of DNA damage both in tumor cells and PBLs were not related to the degree of tumor differentiation as well as the depth of myometrial invasion, but depended on the body mass index (BMI) of EC patients: high level of lesions was observed in patients with elevated BMI values. Furthermore, the level of DNA damage in tumor cells was associated to familial aggregation of cancer and was significantly higher in endometrial cells from patients with family history of cancer vs that from EC patients with sporadic tumors (32.3 ± 2.9 and 22.8 ± 1.8% tail DNA, respectively) (p < 0.05). It was also found that for women who had high level of DNA damage in PBLs, the risk of EC was greater (odds ratio value of 3.5) compared to those with low level of such lesions. Conclusion: Genome instability that appears as an increased level of DNA damage in tumor cells and PBLs of EC patients is associated with BMI and family history of cancer and can reflect a predisposition to cancer.
format Article
author Buchynska, L.G.
Brieieva, O.V.
Lurchenko, N.P.
Protsenko, V.V.
Nespryadko, S.V.
author_facet Buchynska, L.G.
Brieieva, O.V.
Lurchenko, N.P.
Protsenko, V.V.
Nespryadko, S.V.
author_sort Buchynska, L.G.
title DNA damage in tumor cells and peripheral blood lymphocytes of endometrial cancer patients assessed by the comet assay
title_short DNA damage in tumor cells and peripheral blood lymphocytes of endometrial cancer patients assessed by the comet assay
title_full DNA damage in tumor cells and peripheral blood lymphocytes of endometrial cancer patients assessed by the comet assay
title_fullStr DNA damage in tumor cells and peripheral blood lymphocytes of endometrial cancer patients assessed by the comet assay
title_full_unstemmed DNA damage in tumor cells and peripheral blood lymphocytes of endometrial cancer patients assessed by the comet assay
title_sort dna damage in tumor cells and peripheral blood lymphocytes of endometrial cancer patients assessed by the comet assay
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2017
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/138546
citation_txt DNA damage in tumor cells and peripheral blood lymphocytes of endometrial cancer patients assessed by the comet assay / L.G. Buchynska, O.V. Brieieva, N.P. Iurchenko, V.V. Protsenko, S.V. Nespryadko // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 299–303. — Бібліогр.: 35 назв. — англ.
series Experimental Oncology
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first_indexed 2023-10-18T21:18:24Z
last_indexed 2023-10-18T21:18:24Z
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