Effect of silymarin on N-nitrosodiethylamine induced hepatocarcinogenesis in rats

• Видавець: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
• Дата: 2007
• Автори: Ramakrishnan, G., Augustine, T.A., Jagan, S., Vinodhkumar, R., Devaki, T.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2007
• Назва видання: Experimental Oncology
• Теми: Original contributions
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/138564
• Цитувати: Effect of silymarin on N-nitrosodiethylamine induced hepatocarcinogenesis in rats / G. Ramakrishnan, T.A. Augustine, S. Jagan, R. Vinodhkumar, T. Devaki // Experimental Oncology. — 2007. — Т. 29, № 1. — С. 39-44, . — Бібліогр.: 45 назв. — англ.

Aim: To study the effect of silymarin on the levels of tumor markers and MDA (malondialdehyde) – DNA adduct formation during N-nitrosodiethylamine induced hepatocellular carcinoma in male Wistar albino rats. Methods: The levels of AFP, CEA and activities of liver marker enzymes in serum, MDA-DNA immunohistochemistry were done according to standard procedures in the control and experimental groups of rats. Results: Hepatocellular carcinoma was evidenced from significant (p < 0.05) increases of alpha-fetoprotein, carcinoembryonic antigen, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, gamma-glutamyltransferase and 5?-nucleotidase in serum and increased MDA-DNA adducts were also observed in the tissue sections of hepatocellular carcinoma. Silymarin treatment significantly attenuated the alteration of these markers and decreased the levels of MDA-DNA adduct formation. Conclusion: Silymarin could be developed as a promising chemotherapeutic adjuvant for the treatment of liver cancer.