Expression of membrane-anchored matrix metalloproteinase inhibitor reversion inducing cysteine rich protein with kazal motifs in murine cell lines

Expression of membrane-anchored matrix metalloproteinase inhibitor reversion inducing cysteine rich protein with kazal motifs in murine cell lines

Aim: It has been demonstrated that the endogenous matrix metalloproteinases (MMPs) inhibitor reversion inducing cysteine rich protein with Kazal motifs (RECK) is a reliable prognostic marker for detecting several types of tumors. However, the RECK expressions in most of the normal and neoplastic tis...

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Видавець:Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Дата:2007
Автори: Takagi, S., Hoshino, Y., Osaki, T., Okumura, M., Fuginaga, T.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2007
Назва видання:Experimental Oncology
Теми:
Original contributions
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/138567
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Цитувати:Expression of membrane-anchored matrix metalloproteinase inhibitor reversion inducing cysteine rich protein with kazal motifs in murine cell lines / S. Takagi, Y. Hoshino, T. Osaki, M. Okumura, T. Fuginaga // Experimental Oncology. — 2007. — Т. 29, № 1. — С. 30–34. — Бібліогр.: 31 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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spelling irk-123456789-1385672018-06-20T03:05:19Z Expression of membrane-anchored matrix metalloproteinase inhibitor reversion inducing cysteine rich protein with kazal motifs in murine cell lines Takagi, S. Hoshino, Y. Osaki, T. Okumura, M. Fuginaga, T. Original contributions Aim: It has been demonstrated that the endogenous matrix metalloproteinases (MMPs) inhibitor reversion inducing cysteine rich protein with Kazal motifs (RECK) is a reliable prognostic marker for detecting several types of tumors. However, the RECK expressions in most of the normal and neoplastic tissues were extremely low, and to measure its expression is quite complicated. The purpose of the present study is to establish an easy method to quantify murine RECK mRNA expression for use in future experimental studies. Subsequently, in order to verify the reliability of the established quantification technique, we examined the change in RECK expression and gelatinase secretion in tumor cells when stimulated by the extracellular matrix. Methods: Several murine tumor cells were used in the present study. The real-time polymerase chain reaction (PCR) method and measurement conditions for murine RECK mRNA were studied using these tumor cells. Gelatinase activities were also examined by gelatin zymography. Results: Murine RECK mRNA expression was accurately quantified using real-time PCR. Among the tumor cells used in the study, osteosarcoma cells showed significantly higher RECK mRNA expression than the others. The RECK expression in the osteosarcoma cells was down-regulated by contact with matrigel-coated culture flasks due to increased secretion of gelatinases. Conclusion: The real-time PCR method employed in our study is useful to quantify RECK expression. Показано, что эндогенный ингибитор матриксных протеиназ (MMП) RECK может служить надежным прогностическим маркером для некоторых типов опухолей, однако его экспрессия в большинстве нормальных и неопластических тканей крайне низкая, поэтому возникают сложности, связанные с детекцией таковой. Цель работы — разработка количественного метода определения экспрессии мРНК для использования в экспериментальных исследованиях. Для дальнейшего подтверждения надежности разработанного метода исследованы изменения экспрессии RECK и секреции желатиназ в опухолевых клетках при стимуляции внеклеточным матриксом. Методы: в работе использовали несколько линий опухолевых клеток мыши, в которых экспрессию мРНК RECK анализировали методом ПЦР в режиме реального времени, активность желатиназ — методом зимографии. Результаты: экспрессию мРНК RECK количественно оценили методом ПЦР в режиме реального времени, причем среди исследованных клеточных линий наиболее высокий уровень экспрессии RECK выявили в клетках остеосаркомы. Экспрессия RECK в клетках остеосаркомы подавлялась при контакте с культуральным пластиком, обработанным матригелем, вследствие повышения секреции желатиназ. Выводы: для количественной оценки экспрессии мРНК RECK может быть использован метод ПЦР в режиме реального времени. 2007 Article Expression of membrane-anchored matrix metalloproteinase inhibitor reversion inducing cysteine rich protein with kazal motifs in murine cell lines / S. Takagi, Y. Hoshino, T. Osaki, M. Okumura, T. Fuginaga // Experimental Oncology. — 2007. — Т. 29, № 1. — С. 30–34. — Бібліогр.: 31 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138567 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Takagi, S.
Hoshino, Y.
Osaki, T.
Okumura, M.
Fuginaga, T.
Expression of membrane-anchored matrix metalloproteinase inhibitor reversion inducing cysteine rich protein with kazal motifs in murine cell lines
Experimental Oncology
description Aim: It has been demonstrated that the endogenous matrix metalloproteinases (MMPs) inhibitor reversion inducing cysteine rich protein with Kazal motifs (RECK) is a reliable prognostic marker for detecting several types of tumors. However, the RECK expressions in most of the normal and neoplastic tissues were extremely low, and to measure its expression is quite complicated. The purpose of the present study is to establish an easy method to quantify murine RECK mRNA expression for use in future experimental studies. Subsequently, in order to verify the reliability of the established quantification technique, we examined the change in RECK expression and gelatinase secretion in tumor cells when stimulated by the extracellular matrix. Methods: Several murine tumor cells were used in the present study. The real-time polymerase chain reaction (PCR) method and measurement conditions for murine RECK mRNA were studied using these tumor cells. Gelatinase activities were also examined by gelatin zymography. Results: Murine RECK mRNA expression was accurately quantified using real-time PCR. Among the tumor cells used in the study, osteosarcoma cells showed significantly higher RECK mRNA expression than the others. The RECK expression in the osteosarcoma cells was down-regulated by contact with matrigel-coated culture flasks due to increased secretion of gelatinases. Conclusion: The real-time PCR method employed in our study is useful to quantify RECK expression.
format Article
author Takagi, S.
Hoshino, Y.
Osaki, T.
Okumura, M.
Fuginaga, T.
author_facet Takagi, S.
Hoshino, Y.
Osaki, T.
Okumura, M.
Fuginaga, T.
author_sort Takagi, S.
title Expression of membrane-anchored matrix metalloproteinase inhibitor reversion inducing cysteine rich protein with kazal motifs in murine cell lines
title_short Expression of membrane-anchored matrix metalloproteinase inhibitor reversion inducing cysteine rich protein with kazal motifs in murine cell lines
title_full Expression of membrane-anchored matrix metalloproteinase inhibitor reversion inducing cysteine rich protein with kazal motifs in murine cell lines
title_fullStr Expression of membrane-anchored matrix metalloproteinase inhibitor reversion inducing cysteine rich protein with kazal motifs in murine cell lines
title_full_unstemmed Expression of membrane-anchored matrix metalloproteinase inhibitor reversion inducing cysteine rich protein with kazal motifs in murine cell lines
title_sort expression of membrane-anchored matrix metalloproteinase inhibitor reversion inducing cysteine rich protein with kazal motifs in murine cell lines
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2007
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/138567
citation_txt Expression of membrane-anchored matrix metalloproteinase inhibitor reversion inducing cysteine rich protein with kazal motifs in murine cell lines / S. Takagi, Y. Hoshino, T. Osaki, M. Okumura, T. Fuginaga // Experimental Oncology. — 2007. — Т. 29, № 1. — С. 30–34. — Бібліогр.: 31 назв. — англ.
series Experimental Oncology
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first_indexed 2023-10-18T21:18:25Z
last_indexed 2023-10-18T21:18:25Z
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