Identification of new DNA markers of endometrial cancer in patients from the Ukrainian population

Identification of new DNA markers of endometrial cancer in patients from the Ukrainian population

Aim: To identify clinically significant molecular markers of endometrial cancer. Materials and Methods: Cancer and normal endometrial tissue samples from 20 patients of the Gynecology Clinic of Odessa State Medical University (Odessa, Ukraine) with confirmed endometrial cancer were compared for SSR...

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Дата:2007
Автори: Domenyuk, V.P., Litovkin, K.V., Verbitskaya, T.G., Dubinina, V.G., Bubnov, V.V.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2007
Назва видання:Experimental Oncology
Теми:
Original contributions
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/138574
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Identification of new DNA markers of endometrial cancer in patients from the Ukrainian population / V.P. Domenyuk, K.V. Litovkin, T.G. Verbitskaya, V.G. Dubinina, V.V. Bubnov // Experimental Oncology. — 2007. — Т. 29, № 2. — С. 152–155. — Бібліогр.: 15 назв. — англ.

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spelling irk-123456789-1385742020-10-11T00:35:43Z Identification of new DNA markers of endometrial cancer in patients from the Ukrainian population Domenyuk, V.P. Litovkin, K.V. Verbitskaya, T.G. Dubinina, V.G. Bubnov, V.V. Original contributions Aim: To identify clinically significant molecular markers of endometrial cancer. Materials and Methods: Cancer and normal endometrial tissue samples from 20 patients of the Gynecology Clinic of Odessa State Medical University (Odessa, Ukraine) with confirmed endometrial cancer were compared for SSR and ISSR polymorphisms. Identified polymorphic fragments from anonymous genome regions situated between microsatellite repeats underwent direct DNA sequencing; analysis of their homology to sequences from human genome database has been performed. Results: No significant variability for the microsatellite loci adjacent to the E2F1, BAX, TCF7L2, C-MYC, WNT1, FES, DCC, P27, THRA, APC, CYP19 and P53 genes was detected. Search for new molecular markers of endometrial cancer within anonymous DNA sequences located between microsatellite repeats revealed 100 bp and 174 bp polymorphic fragments. These fragments were detected correspondingly in 60% and 35% of patients. 100 bp fragment appeared to be homologous to a region within the NFKB gene, 174 bp fragment – to a sequence within the DDR1 gene. Conclusions: NFKB1 and DDR1 genes may be regarded as potential markers for some types of endometrial cancer. This is a first report about possible association of these genes with endometrial cancer. Цель: идентификация клинически значимых маркеров рака эндометрия. Материалы и Методы: проведен анализ SSR- и ISSR-полиморфизма в образцах опухолей и непораженной ткани эндометрия двадцати пациентов, получавших лечение в клинике гинекологии Одесского государственного медицинского университета (Одесса, Украина). Выполнено секвенирование выявленных полиморфных фрагментов ДНК, локализованных в анонимных участках генома между микросателлитными повторами (ISSR-полиморфизм), осуществлен анализ их гомологии с известными участками ДНК из базы данных генома человека. Результаты: не установлено значительной вариабельности микросателлитных повторов, соседствующих с генами E2F1, BAX, TCF7L2, C-MYC, WNT1, FES, DCC, P27, THRA, APC, CYP19 и P53. В процессе поиска новых маркеров рака эндометрия среди анонимных последовательностей ДНК, локализующихся между микросателлитными повторами, выявлены полиморфные фрагменты длиной 100 и 174 пн. Эти фрагменты присутствовали соответственно у 60 и 35% пациентов . Фрагмент длиной 100 пн оказался гомологичным участку гена NFKB1, а фрагмент длиной 174 пн – участку гена DDR1. Выводы: гены NFKB1 и DDR1 могут рассматриваться в качестве потенциальных маркеров некоторых типов рака эндометрия. Это первое сообщение о возможной ассоциации данных генов с опухолями эндометрия. 2007 Article Identification of new DNA markers of endometrial cancer in patients from the Ukrainian population / V.P. Domenyuk, K.V. Litovkin, T.G. Verbitskaya, V.G. Dubinina, V.V. Bubnov // Experimental Oncology. — 2007. — Т. 29, № 2. — С. 152–155. — Бібліогр.: 15 назв. — англ. 1812-9269 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138574 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Domenyuk, V.P.
Litovkin, K.V.
Verbitskaya, T.G.
Dubinina, V.G.
Bubnov, V.V.
Identification of new DNA markers of endometrial cancer in patients from the Ukrainian population
Experimental Oncology
description Aim: To identify clinically significant molecular markers of endometrial cancer. Materials and Methods: Cancer and normal endometrial tissue samples from 20 patients of the Gynecology Clinic of Odessa State Medical University (Odessa, Ukraine) with confirmed endometrial cancer were compared for SSR and ISSR polymorphisms. Identified polymorphic fragments from anonymous genome regions situated between microsatellite repeats underwent direct DNA sequencing; analysis of their homology to sequences from human genome database has been performed. Results: No significant variability for the microsatellite loci adjacent to the E2F1, BAX, TCF7L2, C-MYC, WNT1, FES, DCC, P27, THRA, APC, CYP19 and P53 genes was detected. Search for new molecular markers of endometrial cancer within anonymous DNA sequences located between microsatellite repeats revealed 100 bp and 174 bp polymorphic fragments. These fragments were detected correspondingly in 60% and 35% of patients. 100 bp fragment appeared to be homologous to a region within the NFKB gene, 174 bp fragment – to a sequence within the DDR1 gene. Conclusions: NFKB1 and DDR1 genes may be regarded as potential markers for some types of endometrial cancer. This is a first report about possible association of these genes with endometrial cancer.
format Article
author Domenyuk, V.P.
Litovkin, K.V.
Verbitskaya, T.G.
Dubinina, V.G.
Bubnov, V.V.
author_facet Domenyuk, V.P.
Litovkin, K.V.
Verbitskaya, T.G.
Dubinina, V.G.
Bubnov, V.V.
author_sort Domenyuk, V.P.
title Identification of new DNA markers of endometrial cancer in patients from the Ukrainian population
title_short Identification of new DNA markers of endometrial cancer in patients from the Ukrainian population
title_full Identification of new DNA markers of endometrial cancer in patients from the Ukrainian population
title_fullStr Identification of new DNA markers of endometrial cancer in patients from the Ukrainian population
title_full_unstemmed Identification of new DNA markers of endometrial cancer in patients from the Ukrainian population
title_sort identification of new dna markers of endometrial cancer in patients from the ukrainian population
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2007
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/138574
citation_txt Identification of new DNA markers of endometrial cancer in patients from the Ukrainian population / V.P. Domenyuk, K.V. Litovkin, T.G. Verbitskaya, V.G. Dubinina, V.V. Bubnov // Experimental Oncology. — 2007. — Т. 29, № 2. — С. 152–155. — Бібліогр.: 15 назв. — англ.
series Experimental Oncology
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AT dubininavg identificationofnewdnamarkersofendometrialcancerinpatientsfromtheukrainianpopulation
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first_indexed 2023-10-18T21:18:46Z
last_indexed 2023-10-18T21:18:46Z
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