Experimental study of the efficacy of combined use of cancer vaccine and interferon

• Дата: 2007
• Автори: Potebnya, G.P., Kudryavets, Yu.Y., Lisovenko, G.S., Cheremshenko, N.L., Voeykova, I.M., Trokhimenko, N.V., Symchich, T.V., Evstrateyva, L.M.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2007
• Назва видання: Experimental Oncology
• Теми: Original contributions
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/138575
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Experimental study of the efficacy of combined use of cancer vaccine and interferon / G.P. Potebnya, Yu.Y. Kudryavets, G.S. Lisovenko, N.L. Cheremshenko, I.M. Voeykova, N.V. Trokhimenko, T.V. Symchich, L.M. Evstrateyva // Experimental Oncology. — 2007. — Т. 29, № 2. — С. 102–105. — Бібліогр.: 23 назв. — англ.

Aim: To study in in vivo model the efficacy of combined scheme of administration of cancer vaccine (CV) and interferon (IFN). Materials and Methods: Lewis lung carcinoma (LLC) was transplanted to male C57Bl mice. For treatment, CV prepared from LLC cells with the use of cytotoxic lectins of B. subtilis B-7025, and preparation of murine IFN-alpha were used. Therapeutic effect was evaluated by measurement of tumor volume and analysis of average life span (ALS) of treated animals. Immunologic study included determination of antitumor cytotoxicity of T-lymphocytes (CTL) and natural killer (NK) cells by radiometric method, functional activity of peritoneal macrophages (MP) — by colorimetric test with nitroazole blue, and evaluation of titers of tumor necrosis factor (TNF) and interleukins-1 and -2 (IL-1, 2). Results: It has been shown that the use of IFN preparation significantly elevated efficacy of vaccine therapy of solid form of LLC: duration of latent period of tumor growth elevated by 25%, ALS — by 28%, index of tumor growth inhibition — by 35–40%. Upon combined use of CV and IFN, significant activation of the cells — effectors of nonspecific immune defense (MP), and specific one (CTL) was observed. Conclusion: The obtained results evidence on perspectiveness of the development of combined schemes of administration of CV and IFN for elevation of the efficacy of vaccine therapy.