Experimental study of the efficacy of combined use of cancer vaccine and interferon
Aim: To study in in vivo model the efficacy of combined scheme of administration of cancer vaccine (CV) and interferon (IFN). Materials and Methods: Lewis lung carcinoma (LLC) was transplanted to male C57Bl mice. For treatment, CV prepared from LLC cells with the use of cytotoxic lectins of B. subti...
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Дата: | 2007 |
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Автори: | , , , , , , , |
Формат: | Стаття |
Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2007
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Назва видання: | Experimental Oncology |
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Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/138575 |
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Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
Цитувати: | Experimental study of the efficacy of combined use of cancer vaccine and interferon / G.P. Potebnya, Yu.Y. Kudryavets, G.S. Lisovenko, N.L. Cheremshenko, I.M. Voeykova, N.V. Trokhimenko, T.V. Symchich, L.M. Evstrateyva // Experimental Oncology. — 2007. — Т. 29, № 2. — С. 102–105. — Бібліогр.: 23 назв. — англ. |
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irk-123456789-1385752018-06-20T03:03:28Z Experimental study of the efficacy of combined use of cancer vaccine and interferon Potebnya, G.P. Kudryavets, Yu.Y. Lisovenko, G.S. Cheremshenko, N.L. Voeykova, I.M. Trokhimenko, N.V. Symchich, T.V. Evstrateyva, L.M. Original contributions Aim: To study in in vivo model the efficacy of combined scheme of administration of cancer vaccine (CV) and interferon (IFN). Materials and Methods: Lewis lung carcinoma (LLC) was transplanted to male C57Bl mice. For treatment, CV prepared from LLC cells with the use of cytotoxic lectins of B. subtilis B-7025, and preparation of murine IFN-alpha were used. Therapeutic effect was evaluated by measurement of tumor volume and analysis of average life span (ALS) of treated animals. Immunologic study included determination of antitumor cytotoxicity of T-lymphocytes (CTL) and natural killer (NK) cells by radiometric method, functional activity of peritoneal macrophages (MP) — by colorimetric test with nitroazole blue, and evaluation of titers of tumor necrosis factor (TNF) and interleukins-1 and -2 (IL-1, 2). Results: It has been shown that the use of IFN preparation significantly elevated efficacy of vaccine therapy of solid form of LLC: duration of latent period of tumor growth elevated by 25%, ALS — by 28%, index of tumor growth inhibition — by 35–40%. Upon combined use of CV and IFN, significant activation of the cells — effectors of nonspecific immune defense (MP), and specific one (CTL) was observed. Conclusion: The obtained results evidence on perspectiveness of the development of combined schemes of administration of CV and IFN for elevation of the efficacy of vaccine therapy. Цель: исследовать в эксперименте эффективность комбинированной схемы введения противоопухолевой вакцины (ПВ) и интерферона (ИФН). Материалы и методы: карциному легкого Льюис (КЛЛ) трансплантировали мышам-самцам C57Bl. Для лечения использовали ПВ, приготовленную из клеток КЛЛ с помощью цитотоксических лектинов B. subtilis B-7025, и препарат мышиного ИФН. Терапевтический эффект оценивали путем измерения объема солидной опухоли и анализа средней продолжительности жизни опытных животных. Иммунологическое исследование включало определение противоопухолевой цитотоксичности Т-лимфоцитов (ЦТЛ) и природных киллерных клеток (ПКК) радиометрическим методом; функциональной активности перитонеальных макрофагов (Мф) в колориметрическом НСТ-тесте; определение титров фактора некроза опухоли (ФНО), интерлейкинов-1 и -2. Результаты: показано, что использование препарата ИФН существенно повышает эффективность вакцинотерапии солидной формы модельной КЛЛ: на 25% повышается продолжительность латентного периода, на 28% — средняя продолжительность жизни мышей, на 35–40% — индекс торможения опухолевого роста. При комбинированном применении ПВ и ИФН отмечают существенную активацию клеток-эффекторов как неспецифической (Мф), так и специфической (ЦТЛ) иммунной защиты. Выводы: полученные результаты свидетельствуют о перспективности разработки комбинированных схем введения ПВ с ИФН, позволяющих повысить эффективность вакцинотерапии. 2007 Article Experimental study of the efficacy of combined use of cancer vaccine and interferon / G.P. Potebnya, Yu.Y. Kudryavets, G.S. Lisovenko, N.L. Cheremshenko, I.M. Voeykova, N.V. Trokhimenko, T.V. Symchich, L.M. Evstrateyva // Experimental Oncology. — 2007. — Т. 29, № 2. — С. 102–105. — Бібліогр.: 23 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138575 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine |
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Original contributions Original contributions |
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Original contributions Original contributions Potebnya, G.P. Kudryavets, Yu.Y. Lisovenko, G.S. Cheremshenko, N.L. Voeykova, I.M. Trokhimenko, N.V. Symchich, T.V. Evstrateyva, L.M. Experimental study of the efficacy of combined use of cancer vaccine and interferon Experimental Oncology |
description |
Aim: To study in in vivo model the efficacy of combined scheme of administration of cancer vaccine (CV) and interferon (IFN). Materials and Methods: Lewis lung carcinoma (LLC) was transplanted to male C57Bl mice. For treatment, CV prepared from LLC cells with the use of cytotoxic lectins of B. subtilis B-7025, and preparation of murine IFN-alpha were used. Therapeutic effect was evaluated by measurement of tumor volume and analysis of average life span (ALS) of treated animals. Immunologic study included determination of antitumor cytotoxicity of T-lymphocytes (CTL) and natural killer (NK) cells by radiometric method, functional activity of peritoneal macrophages (MP) — by colorimetric test with nitroazole blue, and evaluation of titers of tumor necrosis factor (TNF) and interleukins-1 and -2 (IL-1, 2). Results: It has been shown that the use of IFN preparation significantly elevated efficacy of vaccine therapy of solid form of LLC: duration of latent period of tumor growth elevated by 25%, ALS — by 28%, index of tumor growth inhibition — by 35–40%. Upon combined use of CV and IFN, significant activation of the cells — effectors of nonspecific immune defense (MP), and specific one (CTL) was observed. Conclusion: The obtained results evidence on perspectiveness of the development of combined schemes of administration of CV and IFN for elevation of the efficacy of vaccine therapy. |
format |
Article |
author |
Potebnya, G.P. Kudryavets, Yu.Y. Lisovenko, G.S. Cheremshenko, N.L. Voeykova, I.M. Trokhimenko, N.V. Symchich, T.V. Evstrateyva, L.M. |
author_facet |
Potebnya, G.P. Kudryavets, Yu.Y. Lisovenko, G.S. Cheremshenko, N.L. Voeykova, I.M. Trokhimenko, N.V. Symchich, T.V. Evstrateyva, L.M. |
author_sort |
Potebnya, G.P. |
title |
Experimental study of the efficacy of combined use of cancer vaccine and interferon |
title_short |
Experimental study of the efficacy of combined use of cancer vaccine and interferon |
title_full |
Experimental study of the efficacy of combined use of cancer vaccine and interferon |
title_fullStr |
Experimental study of the efficacy of combined use of cancer vaccine and interferon |
title_full_unstemmed |
Experimental study of the efficacy of combined use of cancer vaccine and interferon |
title_sort |
experimental study of the efficacy of combined use of cancer vaccine and interferon |
publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
publishDate |
2007 |
topic_facet |
Original contributions |
url |
http://dspace.nbuv.gov.ua/handle/123456789/138575 |
citation_txt |
Experimental study of the efficacy of combined use of cancer vaccine and interferon / G.P. Potebnya, Yu.Y. Kudryavets, G.S. Lisovenko, N.L. Cheremshenko, I.M. Voeykova, N.V. Trokhimenko, T.V. Symchich, L.M. Evstrateyva // Experimental Oncology. — 2007. — Т. 29, № 2. — С. 102–105. — Бібліогр.: 23 назв. — англ. |
series |
Experimental Oncology |
work_keys_str_mv |
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first_indexed |
2023-10-18T21:19:12Z |
last_indexed |
2023-10-18T21:19:12Z |
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