TGF-β1 expression by glioma C6 cells in vitro
The aim of the work was to study the impact of fetal rat brain cell supernatant (FRBCS) on the expression of transforming growth factor β1 (TGF-β1) and p53 in C6 cells of rat glioma in vitro. Materials and Methods: FRBCS was obtained from suspensions of fetal rat brain cells on the 14th (E14) day of...
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| Дата: | 2017 |
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| Автори: | , , , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2017
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/138583 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | TGF-β1 expression by glioma C6 cells in vitro / L.D. Liubich, L.M. Kovalevska, M.I. Lisyany, V.M. Semenova, T.A. Malysheva, L.P. Stayno, V.V. Vaslovych // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 258–263. — Бібліогр.: 40 назв. — англ. |
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irk-123456789-1385832018-06-20T03:07:53Z TGF-β1 expression by glioma C6 cells in vitro Liubich, L.D. Kovalevska, L.M. Lisyany, M.I. Semenova, V.M. Malysheva, T.A. Stayno, L.P. Vaslovych, V.V. Original contributions The aim of the work was to study the impact of fetal rat brain cell supernatant (FRBCS) on the expression of transforming growth factor β1 (TGF-β1) and p53 in C6 cells of rat glioma in vitro. Materials and Methods: FRBCS was obtained from suspensions of fetal rat brain cells on the 14th (E14) day of gestation. C6 glioma cells were cultured for 48 h in the presence of FRBCS or FRBCS + anti-TGF-β1 monoclonal antibody. Immunocytochemical staining for TGF-β1 and p53 was performed. Results: The proportion of TGF-β1-immunopositive tumor cells in C6 glioma cultures was statistically significantly higher than in the control cell cultures of normal fetal rat brain. FRBCS reduced the proportion of TGF-β1-immunopositive tumor cells and increased the proportion of p53-immunopositive cells in C6 glioma cultures. In cells cultured with FRBCS + anti-TGF-β1 monoclonal antibody, the above effects of FRBCS were abrogated. Conclusion: The obtained results suggest that TGF-β1 seems to be responsible for decrease in TGF-β1 expression and increase in p53 expression in C6 glioma cells treated with FRBCS. 2017 Article TGF-β1 expression by glioma C6 cells in vitro / L.D. Liubich, L.M. Kovalevska, M.I. Lisyany, V.M. Semenova, T.A. Malysheva, L.P. Stayno, V.V. Vaslovych // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 258–263. — Бібліогр.: 40 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138583 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Liubich, L.D. Kovalevska, L.M. Lisyany, M.I. Semenova, V.M. Malysheva, T.A. Stayno, L.P. Vaslovych, V.V. TGF-β1 expression by glioma C6 cells in vitro Experimental Oncology |
| description |
The aim of the work was to study the impact of fetal rat brain cell supernatant (FRBCS) on the expression of transforming growth factor β1 (TGF-β1) and p53 in C6 cells of rat glioma in vitro. Materials and Methods: FRBCS was obtained from suspensions of fetal rat brain cells on the 14th (E14) day of gestation. C6 glioma cells were cultured for 48 h in the presence of FRBCS or FRBCS + anti-TGF-β1 monoclonal antibody. Immunocytochemical staining for TGF-β1 and p53 was performed. Results: The proportion of TGF-β1-immunopositive tumor cells in C6 glioma cultures was statistically significantly higher than in the control cell cultures of normal fetal rat brain. FRBCS reduced the proportion of TGF-β1-immunopositive tumor cells and increased the proportion of p53-immunopositive cells in C6 glioma cultures. In cells cultured with FRBCS + anti-TGF-β1 monoclonal antibody, the above effects of FRBCS were abrogated. Conclusion: The obtained results suggest that TGF-β1 seems to be responsible for decrease in TGF-β1 expression and increase in p53 expression in C6 glioma cells treated with FRBCS. |
| format |
Article |
| author |
Liubich, L.D. Kovalevska, L.M. Lisyany, M.I. Semenova, V.M. Malysheva, T.A. Stayno, L.P. Vaslovych, V.V. |
| author_facet |
Liubich, L.D. Kovalevska, L.M. Lisyany, M.I. Semenova, V.M. Malysheva, T.A. Stayno, L.P. Vaslovych, V.V. |
| author_sort |
Liubich, L.D. |
| title |
TGF-β1 expression by glioma C6 cells in vitro |
| title_short |
TGF-β1 expression by glioma C6 cells in vitro |
| title_full |
TGF-β1 expression by glioma C6 cells in vitro |
| title_fullStr |
TGF-β1 expression by glioma C6 cells in vitro |
| title_full_unstemmed |
TGF-β1 expression by glioma C6 cells in vitro |
| title_sort |
tgf-β1 expression by glioma c6 cells in vitro |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2017 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/138583 |
| citation_txt |
TGF-β1 expression by glioma C6 cells in vitro / L.D. Liubich, L.M. Kovalevska, M.I. Lisyany, V.M. Semenova, T.A. Malysheva, L.P. Stayno, V.V. Vaslovych // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 258–263. — Бібліогр.: 40 назв. — англ. |
| series |
Experimental Oncology |
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