Immunocytochemical study of BCR and bcr-abl localization in K562 cells
Aim: To obtain polyclonal antibodies against recombinant proteins recognizing Bcr domain and fusion region of Bcr-Abl and analyze the patterns of intracellular distribution of Bcr and Bcr-Abl proteins in K562 cells of chronic myelogenous leukemia. Methods: The coding sequences of DН and РН domains o...
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| Дата: | 2010 |
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| Автори: | , , , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2010
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/138603 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Immunocytochemical study of BCR and bcr-abl localization in K562 cells / G.D. Telegeev, A.N. Dubrovska, V.A. Nadgorna, M.V. Dybkov, M.P. Zavelevich, S.S. Maliuta, D.F. Gluzman // Experimental Oncology. — 2010. — Т. 32, № 2. — С. 81-83. — Бібліогр.: 13 назв. — англ. |
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irk-123456789-1386032018-06-20T03:07:14Z Immunocytochemical study of BCR and bcr-abl localization in K562 cells Telegeev, G.D. Dubrovska, A.N. Nadgorna, V.A. Dybkov, M.V. Zavelevich, M.P. Maliuta, S.S. Gluzman, D.F. Original contributions Aim: To obtain polyclonal antibodies against recombinant proteins recognizing Bcr domain and fusion region of Bcr-Abl and analyze the patterns of intracellular distribution of Bcr and Bcr-Abl proteins in K562 cells of chronic myelogenous leukemia. Methods: The coding sequences of DН and РН domains of Bcr-Abl were cloned, and the recombinant proteins were expressed in E. coli. The rabbit polyclonal antibodies were produced and used for immunocytochemical study of Bcr and Bcr-Abl localization in K562 cells. Results: The gene constructs containing sequences coding for DН and РН domains of Bcr-Abl have been obtained. The antibodies with relative specificity to corresponding recombinant proteins differ by the patterns of their intracellular reactivity with Bcr- and Bcr-Abl related structures. While Bcr protein is located predominantly perinuclearly, antibody against hybrid Bcr-Abl protein is reacted with the structures in cell periphery, namely on cell membranes. Conclusion: Antibodies against DН and РН domains of Bcr-Abl react with proteins located differently in chronic myelogenous leukemia cells. The difference in intracellular localization of Bcr and Bcr-Abl may be attributable to the different domains interacting with different multiprotein complexes. 2010 Article Immunocytochemical study of BCR and bcr-abl localization in K562 cells / G.D. Telegeev, A.N. Dubrovska, V.A. Nadgorna, M.V. Dybkov, M.P. Zavelevich, S.S. Maliuta, D.F. Gluzman // Experimental Oncology. — 2010. — Т. 32, № 2. — С. 81-83. — Бібліогр.: 13 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138603 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Telegeev, G.D. Dubrovska, A.N. Nadgorna, V.A. Dybkov, M.V. Zavelevich, M.P. Maliuta, S.S. Gluzman, D.F. Immunocytochemical study of BCR and bcr-abl localization in K562 cells Experimental Oncology |
| description |
Aim: To obtain polyclonal antibodies against recombinant proteins recognizing Bcr domain and fusion region of Bcr-Abl and analyze the patterns of intracellular distribution of Bcr and Bcr-Abl proteins in K562 cells of chronic myelogenous leukemia. Methods: The coding sequences of DН and РН domains of Bcr-Abl were cloned, and the recombinant proteins were expressed in E. coli. The rabbit polyclonal antibodies were produced and used for immunocytochemical study of Bcr and Bcr-Abl localization in K562 cells. Results: The gene constructs containing sequences coding for DН and РН domains of Bcr-Abl have been obtained. The antibodies with relative specificity to corresponding recombinant proteins differ by the patterns of their intracellular reactivity with Bcr- and Bcr-Abl related structures. While Bcr protein is located predominantly perinuclearly, antibody against hybrid Bcr-Abl protein is reacted with the structures in cell periphery, namely on cell membranes. Conclusion: Antibodies against DН and РН domains of Bcr-Abl react with proteins located differently in chronic myelogenous leukemia cells. The difference in intracellular localization of Bcr and Bcr-Abl may be attributable to the different domains interacting with different multiprotein complexes. |
| format |
Article |
| author |
Telegeev, G.D. Dubrovska, A.N. Nadgorna, V.A. Dybkov, M.V. Zavelevich, M.P. Maliuta, S.S. Gluzman, D.F. |
| author_facet |
Telegeev, G.D. Dubrovska, A.N. Nadgorna, V.A. Dybkov, M.V. Zavelevich, M.P. Maliuta, S.S. Gluzman, D.F. |
| author_sort |
Telegeev, G.D. |
| title |
Immunocytochemical study of BCR and bcr-abl localization in K562 cells |
| title_short |
Immunocytochemical study of BCR and bcr-abl localization in K562 cells |
| title_full |
Immunocytochemical study of BCR and bcr-abl localization in K562 cells |
| title_fullStr |
Immunocytochemical study of BCR and bcr-abl localization in K562 cells |
| title_full_unstemmed |
Immunocytochemical study of BCR and bcr-abl localization in K562 cells |
| title_sort |
immunocytochemical study of bcr and bcr-abl localization in k562 cells |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2010 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/138603 |
| citation_txt |
Immunocytochemical study of BCR and bcr-abl localization in K562 cells / G.D. Telegeev, A.N. Dubrovska, V.A. Nadgorna, M.V. Dybkov, M.P. Zavelevich, S.S. Maliuta, D.F. Gluzman // Experimental Oncology. — 2010. — Т. 32, № 2. — С. 81-83. — Бібліогр.: 13 назв. — англ. |
| series |
Experimental Oncology |
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| first_indexed |
2023-10-18T21:18:27Z |
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2023-10-18T21:18:27Z |
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