In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1
Long-term survival of patients with hepatocellular carcinoma (HCC), a common cancer worldwide, remains poor, due to metastasis and recurrence. Aim: To investigate the effect of a novel nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract on human HCC cell line Sk-He...
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| Дата: | 2010 |
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| Автори: | , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2010
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/138604 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 / M.W. Roomi, N.W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2010. — Т. 32, № 2. — С. 84-91. — Бібліогр.: 25 назв. — англ. |
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irk-123456789-1386042018-06-20T03:07:19Z In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 Roomi, M.W. Roomi, N.W. Kalinovsky, T. Niedzwiecki, A. Rath, M. Original contributions Long-term survival of patients with hepatocellular carcinoma (HCC), a common cancer worldwide, remains poor, due to metastasis and recurrence. Aim: To investigate the effect of a novel nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract on human HCC cell line Sk-Hep-1 In vivo and In vitro. Methods: After one week of isolation, 5–6 week old male athymic nude mice were inoculated with 3 x 106 SK-Hep-1 cells subcutaneously and randomly divided into two groups; group A was fed a regular diet and group B a regular diet supplemented with 0.5% NM. Four weeks later, the mice were sacrificed and their tumors were excised, weighed and processed for histology. We also tested the effect of NM In vitro on SK-Hep-1 cells, measuring cell proliferation by MTT assay, invasion through Matrigel, apoptosis by green caspase detection kit, MMP secretion by zymography, and morphology by H&E staining. Results: NM inhibited tumor weight and burden of SK-Hep-1 xenografts by 42% and 33% respectively. In vitro, NM exhibited 33% toxicity over the control at 500 and 1000 μg/ml concentration. Zymography demonstrated MMP-2 and MMP-9 secretion which was inhibited by NM in a dose dependent fashion, with virtual total inhibition at 1000 μg/ml. Invasion through Matrigel was inhibited at 100, 500 and 1000 μg/ml by 53%, 83% and 100% respectively. NM induced slight apoptosis at 100 μg/ml, and profound apoptosis at 500 μg/ml and 1000 μg/ml concentration. Conclusions: These results suggest that NM has therapeutic potential in treatment of HCC. 2010 Article In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 / M.W. Roomi, N.W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2010. — Т. 32, № 2. — С. 84-91. — Бібліогр.: 25 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138604 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Roomi, M.W. Roomi, N.W. Kalinovsky, T. Niedzwiecki, A. Rath, M. In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 Experimental Oncology |
| description |
Long-term survival of patients with hepatocellular carcinoma (HCC), a common cancer worldwide, remains poor, due to metastasis and recurrence. Aim: To investigate the effect of a novel nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract on human HCC cell line Sk-Hep-1 In vivo and In vitro. Methods: After one week of isolation, 5–6 week old male athymic nude mice were inoculated with 3 x 106 SK-Hep-1 cells subcutaneously and randomly divided into two groups; group A was fed a regular diet and group B a regular diet supplemented with 0.5% NM. Four weeks later, the mice were sacrificed and their tumors were excised, weighed and processed for histology. We also tested the effect of NM In vitro on SK-Hep-1 cells, measuring cell proliferation by MTT assay, invasion through Matrigel, apoptosis by green caspase detection kit, MMP secretion by zymography, and morphology by H&E staining. Results: NM inhibited tumor weight and burden of SK-Hep-1 xenografts by 42% and 33% respectively. In vitro, NM exhibited 33% toxicity over the control at 500 and 1000 μg/ml concentration. Zymography demonstrated MMP-2 and MMP-9 secretion which was inhibited by NM in a dose dependent fashion, with virtual total inhibition at 1000 μg/ml. Invasion through Matrigel was inhibited at 100, 500 and 1000 μg/ml by 53%, 83% and 100% respectively. NM induced slight apoptosis at 100 μg/ml, and profound apoptosis at 500 μg/ml and 1000 μg/ml concentration. Conclusions: These results suggest that NM has therapeutic potential in treatment of HCC. |
| format |
Article |
| author |
Roomi, M.W. Roomi, N.W. Kalinovsky, T. Niedzwiecki, A. Rath, M. |
| author_facet |
Roomi, M.W. Roomi, N.W. Kalinovsky, T. Niedzwiecki, A. Rath, M. |
| author_sort |
Roomi, M.W. |
| title |
In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 |
| title_short |
In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 |
| title_full |
In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 |
| title_fullStr |
In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 |
| title_full_unstemmed |
In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 |
| title_sort |
in vivo and in vitro effect of a nutrient mixture on human hepatocarcinoma cell line sk-hep-1 |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2010 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/138604 |
| citation_txt |
In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 / M.W. Roomi, N.W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2010. — Т. 32, № 2. — С. 84-91. — Бібліогр.: 25 назв. — англ. |
| series |
Experimental Oncology |
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