Tightly-bound to DNA proteins in rat experimental hepatomas and normal liver cells
Proteins tightly bound to DNA (TBP) comprise a group of proteins that remain bound to DNA even after harsh deproteinization procedures. The amount of these proteins is 20–100 µg for mg of DNA depending on eukaryotic source. This experimental paper examines the possibility to use some TBP for clinica...
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| Дата: | 2011 |
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| Автори: | , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2011
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/138657 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Tightly-bound to DNA proteins in rat experimental hepatomas and normal liver cells / D. Labeikyte, V. Borutinskaite, N. Legzdins, N. Sjakste // Experimental Oncology. — 2011. — Т. 33, № 3. — С. 121-125. — Бібліогр.: 30 назв. — англ. |
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irk-123456789-1386572018-06-20T03:08:36Z Tightly-bound to DNA proteins in rat experimental hepatomas and normal liver cells Labeikyte, D. Borutinskaite, V. Legzdins, N. Sjakste, N. Original contributions Proteins tightly bound to DNA (TBP) comprise a group of proteins that remain bound to DNA even after harsh deproteinization procedures. The amount of these proteins is 20–100 µg for mg of DNA depending on eukaryotic source. This experimental paper examines the possibility to use some TBP for clinical biomarker discovery, e.g. for identification of prognostic and diagnostic cancer markers. The main aim of this study was to designate differences between tightly DNA binding protein patterns extracted from rat liver and rat experimental hepatomas (Zajdela ascites hepatoma and hepatoma G-27) and to evaluate possibility that some of these proteins may be used as biomarkers for cell cancer transformation. Methods: We used proteomics aproach as a tool for comparison of pattern of TBP from rat experimental hepatomas and normal liver cells. Combination of 2DE fractionation with mass spectrometry (MALDI TOF-MS) suitable for parallel profiling of complex TBP mixtures. Results: Intriguingly 2DE protein maps of TBP from rat liver and rat experimental hepatomas (Zajdela acites hepatoma and hepatoma G-27) were quite different. We identified 9 proteins, some of them shared in all TBP patterns. Among identified tightly bound to DNA proteins there were three proteins considered as nuclear matrix proteins (lamin B1, scaffold attachment factor B1, heterogeneous nuclear ribonucleoprotein). Also we identified DNA repair protein RAD50, coiled-coil domain-containing protein 41, structural maintenance of chromosomes protein1A and some ATP –dependent RNA helicases indicating that TBP are of interest with respect to their potential involvement in the topological organization and/or function of genomic DNA. Conclusions: We suppose that proteomic approach for TBP identification may be promising in development of biomarkers, also obtained results may be valuable for further understanding TBP functions in genome. 2011 Article Tightly-bound to DNA proteins in rat experimental hepatomas and normal liver cells / D. Labeikyte, V. Borutinskaite, N. Legzdins, N. Sjakste // Experimental Oncology. — 2011. — Т. 33, № 3. — С. 121-125. — Бібліогр.: 30 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138657 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Labeikyte, D. Borutinskaite, V. Legzdins, N. Sjakste, N. Tightly-bound to DNA proteins in rat experimental hepatomas and normal liver cells Experimental Oncology |
| description |
Proteins tightly bound to DNA (TBP) comprise a group of proteins that remain bound to DNA even after harsh deproteinization procedures. The amount of these proteins is 20–100 µg for mg of DNA depending on eukaryotic source. This experimental paper examines the possibility to use some TBP for clinical biomarker discovery, e.g. for identification of prognostic and diagnostic cancer markers. The main aim of this study was to designate differences between tightly DNA binding protein patterns extracted from rat liver and rat experimental hepatomas (Zajdela ascites hepatoma and hepatoma G-27) and to evaluate possibility that some of these proteins may be used as biomarkers for cell cancer transformation. Methods: We used proteomics aproach as a tool for comparison of pattern of TBP from rat experimental hepatomas and normal liver cells. Combination of 2DE fractionation with mass spectrometry (MALDI TOF-MS) suitable for parallel profiling of complex TBP mixtures. Results: Intriguingly 2DE protein maps of TBP from rat liver and rat experimental hepatomas (Zajdela acites hepatoma and hepatoma G-27) were quite different. We identified 9 proteins, some of them shared in all TBP patterns. Among identified tightly bound to DNA proteins there were three proteins considered as nuclear matrix proteins (lamin B1, scaffold attachment factor B1, heterogeneous nuclear ribonucleoprotein). Also we identified DNA repair protein RAD50, coiled-coil domain-containing protein 41, structural maintenance of chromosomes protein1A and some ATP –dependent RNA helicases indicating that TBP are of interest with respect to their potential involvement in the topological organization and/or function of genomic DNA. Conclusions: We suppose that proteomic approach for TBP identification may be promising in development of biomarkers, also obtained results may be valuable for further understanding TBP functions in genome. |
| format |
Article |
| author |
Labeikyte, D. Borutinskaite, V. Legzdins, N. Sjakste, N. |
| author_facet |
Labeikyte, D. Borutinskaite, V. Legzdins, N. Sjakste, N. |
| author_sort |
Labeikyte, D. |
| title |
Tightly-bound to DNA proteins in rat experimental hepatomas and normal liver cells |
| title_short |
Tightly-bound to DNA proteins in rat experimental hepatomas and normal liver cells |
| title_full |
Tightly-bound to DNA proteins in rat experimental hepatomas and normal liver cells |
| title_fullStr |
Tightly-bound to DNA proteins in rat experimental hepatomas and normal liver cells |
| title_full_unstemmed |
Tightly-bound to DNA proteins in rat experimental hepatomas and normal liver cells |
| title_sort |
tightly-bound to dna proteins in rat experimental hepatomas and normal liver cells |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2011 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/138657 |
| citation_txt |
Tightly-bound to DNA proteins in rat experimental hepatomas and normal liver cells / D. Labeikyte, V. Borutinskaite, N. Legzdins, N. Sjakste // Experimental Oncology. — 2011. — Т. 33, № 3. — С. 121-125. — Бібліогр.: 30 назв. — англ. |
| series |
Experimental Oncology |
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2023-10-18T21:18:52Z |
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2023-10-18T21:18:52Z |
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