Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice

Aim: Our main objective was to determine the effect of ascorbate supplementation in mice unable to synthesize ascorbic acid (gulo KO) when challenged with murine B16FO cancer cells. Methods: Gulo KO female mice 36–40 weeks of age were deprived of or maintained on ascorbate in food and water for 4 we...

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Дата:2011
Автори: Cha, J., Roomi, M.W., Ivanov, V., Kalinovsky, T., Niedzwiecki, A., Rath, M.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2011
Назва видання:Experimental Oncology
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Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/138671
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice / J. Cha, M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2011. — Т. 33, № 4. — С. 226-230. — Бібліогр.: 20 назв. — англ.

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spelling irk-123456789-1386712018-06-20T03:10:38Z Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice Cha, J. Roomi, M.W. Ivanov, V. Kalinovsky, T. Niedzwiecki, A. Rath, M. Original contributions Aim: Our main objective was to determine the effect of ascorbate supplementation in mice unable to synthesize ascorbic acid (gulo KO) when challenged with murine B16FO cancer cells. Methods: Gulo KO female mice 36–40 weeks of age were deprived of or maintained on ascorbate in food and water for 4 weeks prior to subcutaneous injection of 2.5×106 B16FO murine melanoma cells in the right flank of mice. A control group of wild type mice were also injected with the melanoma cells and maintained on a regular murine diet. Mice were continued on their respective diets for another 2 weeks after injection. The mice were then sacrificed, blood was drawn and their tumors were measured, excised and processed for histology. Results: Mean weight of animals decreased significantly (30%, p < 0.0001) in the ascorbate-restricted group but increased slightly, but insignificantly, in the ascorbate-supplemented group. The mean tumor weight in ascorbate supplemented mice was significantly reduced (by 64%, p = 0.004) compared to tumor weight in ascorbate-deprived gulo mice. The mean tumor weight of wild type mice did not differ significantly from the ascorbate-supplemented mice. Gulo KO mice supplemented with ascorbate developed smaller tumors with more collagen encapsulation and fibrous capsule interdigitation, while gulo KO mice deprived of ascorbate hosted large tumors with poorly defined borders, showing more necrosis and mitosis. Ascorbate supplementation of gulo KO mice resulted in profoundly decreased serum inflammatory cytokine IL-6 (90% decrease, p = 0.04) and IL-1β (62% decrease) compared to the levels in gulo KO mice deprived of ascorbate. Conclusion: Ascorbate supplementation modulated tumor growth and inflammatory cytokine secretion as well as enhanced encapsulation of tumors in scorbutic mice. 2011 Article Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice / J. Cha, M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2011. — Т. 33, № 4. — С. 226-230. — Бібліогр.: 20 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138671 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Cha, J.
Roomi, M.W.
Ivanov, V.
Kalinovsky, T.
Niedzwiecki, A.
Rath, M.
Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice
Experimental Oncology
description Aim: Our main objective was to determine the effect of ascorbate supplementation in mice unable to synthesize ascorbic acid (gulo KO) when challenged with murine B16FO cancer cells. Methods: Gulo KO female mice 36–40 weeks of age were deprived of or maintained on ascorbate in food and water for 4 weeks prior to subcutaneous injection of 2.5×106 B16FO murine melanoma cells in the right flank of mice. A control group of wild type mice were also injected with the melanoma cells and maintained on a regular murine diet. Mice were continued on their respective diets for another 2 weeks after injection. The mice were then sacrificed, blood was drawn and their tumors were measured, excised and processed for histology. Results: Mean weight of animals decreased significantly (30%, p < 0.0001) in the ascorbate-restricted group but increased slightly, but insignificantly, in the ascorbate-supplemented group. The mean tumor weight in ascorbate supplemented mice was significantly reduced (by 64%, p = 0.004) compared to tumor weight in ascorbate-deprived gulo mice. The mean tumor weight of wild type mice did not differ significantly from the ascorbate-supplemented mice. Gulo KO mice supplemented with ascorbate developed smaller tumors with more collagen encapsulation and fibrous capsule interdigitation, while gulo KO mice deprived of ascorbate hosted large tumors with poorly defined borders, showing more necrosis and mitosis. Ascorbate supplementation of gulo KO mice resulted in profoundly decreased serum inflammatory cytokine IL-6 (90% decrease, p = 0.04) and IL-1β (62% decrease) compared to the levels in gulo KO mice deprived of ascorbate. Conclusion: Ascorbate supplementation modulated tumor growth and inflammatory cytokine secretion as well as enhanced encapsulation of tumors in scorbutic mice.
format Article
author Cha, J.
Roomi, M.W.
Ivanov, V.
Kalinovsky, T.
Niedzwiecki, A.
Rath, M.
author_facet Cha, J.
Roomi, M.W.
Ivanov, V.
Kalinovsky, T.
Niedzwiecki, A.
Rath, M.
author_sort Cha, J.
title Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice
title_short Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice
title_full Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice
title_fullStr Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice
title_full_unstemmed Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice
title_sort ascorbate depletion increases growth and metastasis of melanoma cells in vitamin c deficient mice
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2011
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/138671
citation_txt Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice / J. Cha, M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2011. — Т. 33, № 4. — С. 226-230. — Бібліогр.: 20 назв. — англ.
series Experimental Oncology
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