Nutrient mixture inhibits in vitro and in vivo growth of human acute promyelocytic leukemia HL-60 cells

Nutrient mixture inhibits in vitro and in vivo growth of human acute promyelocytic leukemia HL-60 cells

Aim: Untreated acute promyelocytic leukemia is the most malignant form of acute leukemias, with median survival of less than one month. We investigated in vitro and in vivo synergistic effects of a nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract, on acute promy...

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Дата:2011
Автори: Roomi, M.W., Roomi, N.W., Bhanap, B., Rath, M., Niedzwiecki, A.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2011
Назва видання:Experimental Oncology
Теми:
Original contributions
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/138674
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Nutrient mixture inhibits in vitro and in vivo growth of human acute promyelocytic leukemia HL-60 cells / M.W. Roomi, N.W. Roomi, B. Bhanap, M. Rath, A. Niedzwiecki // Experimental Oncology. — 2011. — Т. 33, № 4. — С. 212-215. — Бібліогр.: 17 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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spelling irk-123456789-1386742018-06-20T03:08:59Z Nutrient mixture inhibits in vitro and in vivo growth of human acute promyelocytic leukemia HL-60 cells Roomi, M.W. Roomi, N.W. Bhanap, B. Rath, M. Niedzwiecki, A. Original contributions Aim: Untreated acute promyelocytic leukemia is the most malignant form of acute leukemias, with median survival of less than one month. We investigated in vitro and in vivo synergistic effects of a nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract, on acute promyelocytic leukemia HL-60 cells. Methods: In vitro, the HL-60 cells were cultured and exposed to NM at doses 0–1000 μg/ml. Cell viability was assessed by Trypan blue dye exclusion test, matrix metalloproteinases (MMP) expression by gelatinase zymography, invasion through Matrigel and apoptosis by live green Poly Caspase Detection Kit. In vivo studies were carried out in athymic nude mice subcutaneously inoculated with HL-60 cells. Results: In vitro, NM exhibited a dose dependent reduction in cells viability. Zymography revealed matrix MMP-2 and phorbol 12-myristate 13-acetate induced MMP-9 expression. NM inhibited expression of both MMP in a dose dependent manner. Similar step-wise reduction in the Matrigel invasion by HL-60 cells was also observed by this combination with incremental doses. Gradually increasing doses of NM induced significant apoptosis in HL-60 cells. In vivo, NM inhibited tumor growth by 50%. Conclusion: The results indicate that NM significantly suppresses tumor growth, decreases cell viability, inhibits MMP expression, Matrigel invasion and induces apoptosis in HL-60 cells. 2011 Article Nutrient mixture inhibits in vitro and in vivo growth of human acute promyelocytic leukemia HL-60 cells / M.W. Roomi, N.W. Roomi, B. Bhanap, M. Rath, A. Niedzwiecki // Experimental Oncology. — 2011. — Т. 33, № 4. — С. 212-215. — Бібліогр.: 17 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138674 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Roomi, M.W.
Roomi, N.W.
Bhanap, B.
Rath, M.
Niedzwiecki, A.
Nutrient mixture inhibits in vitro and in vivo growth of human acute promyelocytic leukemia HL-60 cells
Experimental Oncology
description Aim: Untreated acute promyelocytic leukemia is the most malignant form of acute leukemias, with median survival of less than one month. We investigated in vitro and in vivo synergistic effects of a nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract, on acute promyelocytic leukemia HL-60 cells. Methods: In vitro, the HL-60 cells were cultured and exposed to NM at doses 0–1000 μg/ml. Cell viability was assessed by Trypan blue dye exclusion test, matrix metalloproteinases (MMP) expression by gelatinase zymography, invasion through Matrigel and apoptosis by live green Poly Caspase Detection Kit. In vivo studies were carried out in athymic nude mice subcutaneously inoculated with HL-60 cells. Results: In vitro, NM exhibited a dose dependent reduction in cells viability. Zymography revealed matrix MMP-2 and phorbol 12-myristate 13-acetate induced MMP-9 expression. NM inhibited expression of both MMP in a dose dependent manner. Similar step-wise reduction in the Matrigel invasion by HL-60 cells was also observed by this combination with incremental doses. Gradually increasing doses of NM induced significant apoptosis in HL-60 cells. In vivo, NM inhibited tumor growth by 50%. Conclusion: The results indicate that NM significantly suppresses tumor growth, decreases cell viability, inhibits MMP expression, Matrigel invasion and induces apoptosis in HL-60 cells.
format Article
author Roomi, M.W.
Roomi, N.W.
Bhanap, B.
Rath, M.
Niedzwiecki, A.
author_facet Roomi, M.W.
Roomi, N.W.
Bhanap, B.
Rath, M.
Niedzwiecki, A.
author_sort Roomi, M.W.
title Nutrient mixture inhibits in vitro and in vivo growth of human acute promyelocytic leukemia HL-60 cells
title_short Nutrient mixture inhibits in vitro and in vivo growth of human acute promyelocytic leukemia HL-60 cells
title_full Nutrient mixture inhibits in vitro and in vivo growth of human acute promyelocytic leukemia HL-60 cells
title_fullStr Nutrient mixture inhibits in vitro and in vivo growth of human acute promyelocytic leukemia HL-60 cells
title_full_unstemmed Nutrient mixture inhibits in vitro and in vivo growth of human acute promyelocytic leukemia HL-60 cells
title_sort nutrient mixture inhibits in vitro and in vivo growth of human acute promyelocytic leukemia hl-60 cells
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2011
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/138674
citation_txt Nutrient mixture inhibits in vitro and in vivo growth of human acute promyelocytic leukemia HL-60 cells / M.W. Roomi, N.W. Roomi, B. Bhanap, M. Rath, A. Niedzwiecki // Experimental Oncology. — 2011. — Т. 33, № 4. — С. 212-215. — Бібліогр.: 17 назв. — англ.
series Experimental Oncology
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AT rathm nutrientmixtureinhibitsinvitroandinvivogrowthofhumanacutepromyelocyticleukemiahl60cells
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first_indexed 2023-10-18T21:18:58Z
last_indexed 2023-10-18T21:18:58Z
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