Effects of IL-6, IL-10 and TGF-β on the expression of survivin and apoptosisin nasopharyngeal carcinoma TW01 cells
The aim of this study is to investigate whether IL-6, IL-10 and TGF-β are able to confer resistance to apoptosis in nasopharyngeal carcinoma cells by upregulating the expression of survivin. Methods: The human nasopharyngeal carcinoma cell line TW01 (WHO NPC Type I) was cultured in DMEM-F12 Ham medi...
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| Дата: | 2012 |
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| Автори: | , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2012
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/138694 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Effects of IL-6, IL-10 and TGF-β on the expression of survivin and apoptosisin nasopharyngeal carcinoma TW01 cells / Y.W. Poh, S.Y. Gan, E.L. Tan // Experimental Oncology. — 2012. — Т. 34, № 2. — С. 85-89. — Бібліогр.: 40 назв. — англ. |
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irk-123456789-1386942018-06-20T03:08:41Z Effects of IL-6, IL-10 and TGF-β on the expression of survivin and apoptosisin nasopharyngeal carcinoma TW01 cells Poh, Y.W. Gan, S.Y. Tan, E.L. Original contributions The aim of this study is to investigate whether IL-6, IL-10 and TGF-β are able to confer resistance to apoptosis in nasopharyngeal carcinoma cells by upregulating the expression of survivin. Methods: The human nasopharyngeal carcinoma cell line TW01 (WHO NPC Type I) was cultured in DMEM-F12 Ham medium containing 10% FBS in a humidified atmosphere of 5% CO2 and 37◦C and treated with different concentrations of IL-6, IL-10 and TGF-β. Survivin mRNA expression was measured by real-time quantitative PCR and Western blot. Apoptosis was determined based on the assay for caspase-3 activity. Results: Of all the cytokines tested, only TGF-β (10 pg/mL) induced the over-expression of survivin at a significant level and this correlated with resistance to apoptosis (p ≤ 0.05). To confirm if survivin is responsible for resistance to apoptosis, YM155 which is a survivin inhibitor was used and the results showed that YM155 abrogated the protective effect of TGF-β. Interestingly, IL-10 did not significantly alter the expression of survivin. Conclusions: We conclude that TGF-β up-regulates the expression of survivin leading to the resistance to apoptosis in NPC TW01 cells. 2012 Article Effects of IL-6, IL-10 and TGF-β on the expression of survivin and apoptosisin nasopharyngeal carcinoma TW01 cells / Y.W. Poh, S.Y. Gan, E.L. Tan // Experimental Oncology. — 2012. — Т. 34, № 2. — С. 85-89. — Бібліогр.: 40 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138694 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Poh, Y.W. Gan, S.Y. Tan, E.L. Effects of IL-6, IL-10 and TGF-β on the expression of survivin and apoptosisin nasopharyngeal carcinoma TW01 cells Experimental Oncology |
| description |
The aim of this study is to investigate whether IL-6, IL-10 and TGF-β are able to confer resistance to apoptosis in nasopharyngeal carcinoma cells by upregulating the expression of survivin. Methods: The human nasopharyngeal carcinoma cell line TW01 (WHO NPC Type I) was cultured in DMEM-F12 Ham medium containing 10% FBS in a humidified atmosphere of 5% CO2 and 37◦C and treated with different concentrations of IL-6, IL-10 and TGF-β. Survivin mRNA expression was measured by real-time quantitative PCR and Western blot. Apoptosis was determined based on the assay for caspase-3 activity. Results: Of all the cytokines tested, only TGF-β (10 pg/mL) induced the over-expression of survivin at a significant level and this correlated with resistance to apoptosis (p ≤ 0.05). To confirm if survivin is responsible for resistance to apoptosis, YM155 which is a survivin inhibitor was used and the results showed that YM155 abrogated the protective effect of TGF-β. Interestingly, IL-10 did not significantly alter the expression of survivin. Conclusions: We conclude that TGF-β up-regulates the expression of survivin leading to the resistance to apoptosis in NPC TW01 cells. |
| format |
Article |
| author |
Poh, Y.W. Gan, S.Y. Tan, E.L. |
| author_facet |
Poh, Y.W. Gan, S.Y. Tan, E.L. |
| author_sort |
Poh, Y.W. |
| title |
Effects of IL-6, IL-10 and TGF-β on the expression of survivin and apoptosisin nasopharyngeal carcinoma TW01 cells |
| title_short |
Effects of IL-6, IL-10 and TGF-β on the expression of survivin and apoptosisin nasopharyngeal carcinoma TW01 cells |
| title_full |
Effects of IL-6, IL-10 and TGF-β on the expression of survivin and apoptosisin nasopharyngeal carcinoma TW01 cells |
| title_fullStr |
Effects of IL-6, IL-10 and TGF-β on the expression of survivin and apoptosisin nasopharyngeal carcinoma TW01 cells |
| title_full_unstemmed |
Effects of IL-6, IL-10 and TGF-β on the expression of survivin and apoptosisin nasopharyngeal carcinoma TW01 cells |
| title_sort |
effects of il-6, il-10 and tgf-β on the expression of survivin and apoptosisin nasopharyngeal carcinoma tw01 cells |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2012 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/138694 |
| citation_txt |
Effects of IL-6, IL-10 and TGF-β on the expression of survivin and apoptosisin nasopharyngeal carcinoma TW01 cells / Y.W. Poh, S.Y. Gan, E.L. Tan // Experimental Oncology. — 2012. — Т. 34, № 2. — С. 85-89. — Бібліогр.: 40 назв. — англ. |
| series |
Experimental Oncology |
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| first_indexed |
2023-10-18T21:19:16Z |
| last_indexed |
2023-10-18T21:19:16Z |
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