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Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo

Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo

The aim of the work was the synthesis of gold nanoparticles (GNP) of different sizes and the estimation of their biological activity in vitro and in vivo. Materials and Methods: Water dispersions of gold nanoparticles of different sizes have been synthesized by Davis method and characterized by lase...

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Main Authors: Rieznichenko, L.S., Dybkova, S.M., Gruzina, T.G., Ulberg, Z.R., Todor, I.N., Lukyanova, N.Yu., Shpyleva, S.I., Chekhun, V.F.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2012
Series:Experimental Oncology
Subjects:
Original contributions
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/138708
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spelling irk-123456789-1387082018-06-20T03:06:13Z Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo Rieznichenko, L.S. Dybkova, S.M. Gruzina, T.G. Ulberg, Z.R. Todor, I.N. Lukyanova, N.Yu. Shpyleva, S.I. Chekhun, V.F. Original contributions The aim of the work was the synthesis of gold nanoparticles (GNP) of different sizes and the estimation of their biological activity in vitro and in vivo. Materials and Methods: Water dispersions of gold nanoparticles of different sizes have been synthesized by Davis method and characterized by laser-correlation spectroscopy and transmission electron microscopy methods. The GNP interaction with tumor cells has been visualized by confocal microscopy method. The enzyme activity was determined by standard biochemical methods. GNP distribution and content in organs and tissues have been determined via atomic-absorption spectrometry method; genotoxic influence has been estimated by “Comet-assay” method. Results: The GNP size-dependent accumulation in cultured U937 tumor cells and their ability to modulate U937 cell membrane Na+,K+-АТР-ase activity value has been revealed in vitro. Using in vivo model of Guerin carcinoma it has been shown that GNP possess high affinity to tumor cells. Conclusions: Our results indicate the perspectives of use of the synthesized GNP water dispersions for cancer diagnostics and treatment. It’s necessary to take into account a size-dependent biosafety level of nanoparticles. 2012 Article Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo / L.S. Rieznichenko, S.M. Dybkova, T.G. Gruzina, Z.R. Ulberg, I.N. Todor, N.Yu. Lukyanova, S.I. Shpyleva, V.F. Chekhun // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 25-28. — Бібліогр.: 34 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138708 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Rieznichenko, L.S.
Dybkova, S.M.
Gruzina, T.G.
Ulberg, Z.R.
Todor, I.N.
Lukyanova, N.Yu.
Shpyleva, S.I.
Chekhun, V.F.
Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo
Experimental Oncology
description The aim of the work was the synthesis of gold nanoparticles (GNP) of different sizes and the estimation of their biological activity in vitro and in vivo. Materials and Methods: Water dispersions of gold nanoparticles of different sizes have been synthesized by Davis method and characterized by laser-correlation spectroscopy and transmission electron microscopy methods. The GNP interaction with tumor cells has been visualized by confocal microscopy method. The enzyme activity was determined by standard biochemical methods. GNP distribution and content in organs and tissues have been determined via atomic-absorption spectrometry method; genotoxic influence has been estimated by “Comet-assay” method. Results: The GNP size-dependent accumulation in cultured U937 tumor cells and their ability to modulate U937 cell membrane Na+,K+-АТР-ase activity value has been revealed in vitro. Using in vivo model of Guerin carcinoma it has been shown that GNP possess high affinity to tumor cells. Conclusions: Our results indicate the perspectives of use of the synthesized GNP water dispersions for cancer diagnostics and treatment. It’s necessary to take into account a size-dependent biosafety level of nanoparticles.
format Article
author Rieznichenko, L.S.
Dybkova, S.M.
Gruzina, T.G.
Ulberg, Z.R.
Todor, I.N.
Lukyanova, N.Yu.
Shpyleva, S.I.
Chekhun, V.F.
author_facet Rieznichenko, L.S.
Dybkova, S.M.
Gruzina, T.G.
Ulberg, Z.R.
Todor, I.N.
Lukyanova, N.Yu.
Shpyleva, S.I.
Chekhun, V.F.
author_sort Rieznichenko, L.S.
title Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo
title_short Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo
title_full Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo
title_fullStr Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo
title_full_unstemmed Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo
title_sort gold nanoparticles synthesis and biological activity estimation in vitro and in vivo
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2012
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/138708
citation_txt Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo / L.S. Rieznichenko, S.M. Dybkova, T.G. Gruzina, Z.R. Ulberg, I.N. Todor, N.Yu. Lukyanova, S.I. Shpyleva, V.F. Chekhun // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 25-28. — Бібліогр.: 34 назв. — англ.
series Experimental Oncology
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first_indexed 2023-10-18T21:19:17Z
last_indexed 2023-10-18T21:19:17Z
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