Paclitaxel-octreotide conjugates inhibit growth of human non-small cell lung cancer cells in vitro
Aim: To evaluate the effects of paclitaxel-octreotide conjugates on the growth of cultured non-small cell lung cancer cells. Methods: RT-PCR was performed to detect mRNA for the subtypes of the human somatostatin receptor (SSTR) using specific primers. MTT-based cytotoxicity assay was used to evalua...
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| Дата: | 2007 |
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| Автори: | , , , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2007
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/138959 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Paclitaxel-octreotide conjugates inhibit growth of human non-small cell lung cancer cells in vitro / M.L. Sun, J.M. Wei, X.W. Wang, L. Li, P. Wang, M. Li, C.H. Yi // Experimental Oncology. — 2007. — Т. 29, № 3. — С. 186–191. — Бібліогр.: 32 назв. — англ. |
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irk-123456789-1389592018-06-20T03:06:04Z Paclitaxel-octreotide conjugates inhibit growth of human non-small cell lung cancer cells in vitro Sun, M.L. Wei, J.M. Wang, X.M. Li, L. Wang, P. Li, M. Yi, C.H. Original contributions Aim: To evaluate the effects of paclitaxel-octreotide conjugates on the growth of cultured non-small cell lung cancer cells. Methods: RT-PCR was performed to detect mRNA for the subtypes of the human somatostatin receptor (SSTR) using specific primers. MTT-based cytotoxicity assay was used to evaluate the cell viability after treatment with paclitaxel and the conjugates. Cell cycle perturbations were determined using a Fluorescence-Activated Cell Sorter. Results: Non-small cell lung cancer A549 and Calu-6 cells expressed mRNA for SSTR2 and SSTR5. Paclitaxel and the conjugates effectively inhibited the growth of A549 and Calu-6 cells in a concentration- and time-dependent manner. In SSTR-negative fibroblasts, the conjugates were less cytotoxic than paclitaxel. The conjugates and paclitaxel could induce the increase of G2/M phase ratio in A549 cells. Conclusion: The paclitaxel-octreotide conjugates can be used as selective-targeted chemotherapeutic agents for treating non-small cell lung cancer. Цель: оценить эффект конъюгатов паклитаксела-октреотида на рост культивированных клеток немелкоклеточного рака легкого человека. Методы: для определения мРНК подтипов рецептора соматостатина человека (SSTR) применяли ОT-ПЦР. Анализ цитотоксичности в МТТ-тесте применяли для оценки выживаемости клеток после их инкубации с паклитакселом и конъюгатами. Нарушения клеточного цикла определяли с применением FACS — клеточного сортера. Результаты: установлено, что клеточные линии немелкоклеточного рака легкого A549 и Calu-6 экспрессируют SSTR2 и SSTR5 мРНК. Отмечено эффективное дозо- и времязависимое угнетение роста клеток A549 и Calu-6 паклитакселом и конъюгатами. Для SSTR-негативных фибробластов конъюгаты менее цитотоксичны, чем паклитаксел. Конъюгаты и паклитаксел могут индуцировать повышение соотношения фаз G2 /M в клетках A549. Выводы: конъюгаты паклитаксел-октреотида могут быть использованы как селективные химиотерапевтические агенты для воздействия на немелкоклеточный рак легкого. 2007 Article Paclitaxel-octreotide conjugates inhibit growth of human non-small cell lung cancer cells in vitro / M.L. Sun, J.M. Wei, X.W. Wang, L. Li, P. Wang, M. Li, C.H. Yi // Experimental Oncology. — 2007. — Т. 29, № 3. — С. 186–191. — Бібліогр.: 32 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/138959 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Sun, M.L. Wei, J.M. Wang, X.M. Li, L. Wang, P. Li, M. Yi, C.H. Paclitaxel-octreotide conjugates inhibit growth of human non-small cell lung cancer cells in vitro Experimental Oncology |
| description |
Aim: To evaluate the effects of paclitaxel-octreotide conjugates on the growth of cultured non-small cell lung cancer cells. Methods: RT-PCR was performed to detect mRNA for the subtypes of the human somatostatin receptor (SSTR) using specific primers. MTT-based cytotoxicity assay was used to evaluate the cell viability after treatment with paclitaxel and the conjugates. Cell cycle perturbations were determined using a Fluorescence-Activated Cell Sorter. Results: Non-small cell lung cancer A549 and Calu-6 cells expressed mRNA for SSTR2 and SSTR5. Paclitaxel and the conjugates effectively inhibited the growth of A549 and Calu-6 cells in a concentration- and time-dependent manner. In SSTR-negative fibroblasts, the conjugates were less cytotoxic than paclitaxel. The conjugates and paclitaxel could induce the increase of G2/M phase ratio in A549 cells. Conclusion: The paclitaxel-octreotide conjugates can be used as selective-targeted chemotherapeutic agents for treating non-small cell lung cancer. |
| format |
Article |
| author |
Sun, M.L. Wei, J.M. Wang, X.M. Li, L. Wang, P. Li, M. Yi, C.H. |
| author_facet |
Sun, M.L. Wei, J.M. Wang, X.M. Li, L. Wang, P. Li, M. Yi, C.H. |
| author_sort |
Sun, M.L. |
| title |
Paclitaxel-octreotide conjugates inhibit growth of human non-small cell lung cancer cells in vitro |
| title_short |
Paclitaxel-octreotide conjugates inhibit growth of human non-small cell lung cancer cells in vitro |
| title_full |
Paclitaxel-octreotide conjugates inhibit growth of human non-small cell lung cancer cells in vitro |
| title_fullStr |
Paclitaxel-octreotide conjugates inhibit growth of human non-small cell lung cancer cells in vitro |
| title_full_unstemmed |
Paclitaxel-octreotide conjugates inhibit growth of human non-small cell lung cancer cells in vitro |
| title_sort |
paclitaxel-octreotide conjugates inhibit growth of human non-small cell lung cancer cells in vitro |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2007 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/138959 |
| citation_txt |
Paclitaxel-octreotide conjugates inhibit growth of human non-small cell lung cancer cells in vitro / M.L. Sun, J.M. Wei, X.W. Wang, L. Li, P. Wang, M. Li, C.H. Yi // Experimental Oncology. — 2007. — Т. 29, № 3. — С. 186–191. — Бібліогр.: 32 назв. — англ. |
| series |
Experimental Oncology |
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2023-10-18T21:19:21Z |
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