O₂·- and NO-associated mechanisms of selective action of redox-active cobalt complexes on tumor tissue

• Дата: 2007
• Автори: Ganusevich, I.I., Burlaka, A.P., Sidorik, E.P., Levitin, I.Ya, Sigan, A.L., Osinsky, S.P.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2007
• Назва видання: Experimental Oncology
• Теми: Original contributions
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/138971
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: O₂·- and NO-associated mechanisms of selective action of redox-active cobalt complexes on tumor tissue / I.I. Ganusevich, A.P. Burlaka, E.P. Sidorik, I.Ya. Levitin, A.L. Sigan, S.P. Osinsky // Experimental Oncology. — 2007. — Т. 29, № 3. — С. 203–206. — Бібліогр.: 28 назв. — англ.

Aim: To study the influence of redox-active cobalt(III) complex with tetradentate Schiff base and nicotinamide as an axial ligand on the rate of superoxide radical-anions generation and levels of NO in tumor and normal tissues of Lewis lung carcinoma bearing mice as well as activity of matrix metalloproteinases 2 and 9 (MMPs) in tumor. Methods: The superoxide radical-anions formation and NO level in tissues were assessed by EPR method with the use of 1-hydroxy-2,2,6,6-tetramethyl-4-oxopiperidin and diethyldithiocarbomate spin traps, respectively. MMPs activities were determined by zymography in polyacrylamide gel. Results: It was observed that the rate of superoxide radical-anions generation was selectively increased in tumor tissue (by a factor of 6–7) accompanied with the decrease of NO level (by a factor of 2) due to tested complex administration. Activities of MMPs in tumor were significantly decreased. Conclusion: It is supposed that the one of mechanisms of detected earlier antimetastatic effect of complex is based on its ability to induce the formation of high level of superoxide radical-anions selectively in the tumor tissue that results in the damage of its regulatory functions, in particular alteration in the regulation of NO-synthase, decrease of NO generation as well as activities of MMPs.