Differential expression of 16 genes coding for cell cycle – and apoptosis-related proteins in vitamin D-induced differentiation of HL-60 cells

• Дата: 2007
• Автори: Kanli, A., Savli, H.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2007
• Назва видання: Experimental Oncology
• Теми: Short communications
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/139000
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Differential expression of 16 genes coding for cell cycle – and apoptosis-related proteins in vitamin D-induced differentiation of HL-60 cells / A. Kanli, H. Savli // Experimental Oncology. — 2007. — Т. 29, № 4. — С. 314–316. — Бібліогр.: 19 назв. — англ.

Objective: The aim of the study was to analyze expression of several genes related to cell cycle regulation and apoptosis in realization of 1alpha,25-dihydroxyvitamin D3 (1,25(OH)2D3)-induced differentiation of HL-60 cells. Methods: The cultured HL-60 cells were treated with 1,25(OH)2D3. Quantitative real-time PCR was used for analyzing the changes in expression of 16 genes (Bcl-2, Bcl-xL, Mcl-1, Bik, caspase 6, caspase 7, cytochrome c, TNFR1, Myc, TGF-beta, JNK1, p38MAPK, p21, p27, Cdk2, cyclin E) at early phases of cell differentiation of HL-60 cells induced by 1,25(OH)2D3. Results: Among investigated genes, Bik and Myc gene expression was down-regulated at 48 h time points. JNK1 gene was markedly up-regulated and caspase-6 and cyclin E genes were down-regulated at 18 h time point. Conclusion: These findings suggest that there are no distinct apoptotic signals at early phases of cell differentiation. It is speculated that changes in the expression of the genes involved in vitamin D-induced apoptosis of HL-60 cells could be better visualized after the terminal stages of cell differentiation.