Differential expression of 16 genes coding for cell cycle – and apoptosis-related proteins in vitamin D-induced differentiation of HL-60 cells
Objective: The aim of the study was to analyze expression of several genes related to cell cycle regulation and apoptosis in realization of 1alpha,25-dihydroxyvitamin D3 (1,25(OH)2D3)-induced differentiation of HL-60 cells. Methods: The cultured HL-60 cells were treated with 1,25(OH)2D3. Quantitativ...
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| Дата: | 2007 |
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| Автори: | , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2007
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/139000 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Differential expression of 16 genes coding for cell cycle – and apoptosis-related proteins in vitamin D-induced differentiation of HL-60 cells / A. Kanli, H. Savli // Experimental Oncology. — 2007. — Т. 29, № 4. — С. 314–316. — Бібліогр.: 19 назв. — англ. |
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irk-123456789-1390002018-06-20T03:04:59Z Differential expression of 16 genes coding for cell cycle – and apoptosis-related proteins in vitamin D-induced differentiation of HL-60 cells Kanli, A. Savli, H. Short communications Objective: The aim of the study was to analyze expression of several genes related to cell cycle regulation and apoptosis in realization of 1alpha,25-dihydroxyvitamin D3 (1,25(OH)2D3)-induced differentiation of HL-60 cells. Methods: The cultured HL-60 cells were treated with 1,25(OH)2D3. Quantitative real-time PCR was used for analyzing the changes in expression of 16 genes (Bcl-2, Bcl-xL, Mcl-1, Bik, caspase 6, caspase 7, cytochrome c, TNFR1, Myc, TGF-beta, JNK1, p38MAPK, p21, p27, Cdk2, cyclin E) at early phases of cell differentiation of HL-60 cells induced by 1,25(OH)2D3. Results: Among investigated genes, Bik and Myc gene expression was down-regulated at 48 h time points. JNK1 gene was markedly up-regulated and caspase-6 and cyclin E genes were down-regulated at 18 h time point. Conclusion: These findings suggest that there are no distinct apoptotic signals at early phases of cell differentiation. It is speculated that changes in the expression of the genes involved in vitamin D-induced apoptosis of HL-60 cells could be better visualized after the terminal stages of cell differentiation. Цель: проанализировать экспрессию ряда генов, участвующих в регуляции клеточного цикла и апоптоза в дифференцировке клеток HL-60 промиелоцитарного лейкоза человека, индуцированной 1альфа,25-дигидроксивитамином 3(1,25(OH 2 3). Методы: культивируемые клетки HL-60 инкуировали с 1,25(OH 2 D3. Количественную ПЦР в режиме реального времени использовали для анализа изменений экспрессии 16 генов (Bcl-2, Bc l-xL , l 1, k, каспаза 6, каспаза 7, цитохром с, TNFR1, Myc, TGF- beta, K 1, p38 MAPK, p21, p , Cdk2, иклин Е ) при индукции апоптоза и дифференцировки в клетках HL-60 под дествием 1,25(OH 2 D3. Результаты: среди исследованных генов отмечено снижение экспресси Bik и Myc через 48 ч. Экспрессия гена JNK1 повышена, а генов каспазы-6 и циклина Е снижена в точке 18 ч. Выводы: эти данные свидетельствуют об отсутствии четких сигналов экспрессии апоптоз-ассоциированных генов на ранних фазах клеточной дифференцировки. Предполагается, что изменения в экспрессии генов, участвующих в реализации апоптоза клеток HL-60, индуцированного витаминомD, могут быть выражены лишь на терминальных стадиях дифференцировки этих клеток. 2007 Article Differential expression of 16 genes coding for cell cycle – and apoptosis-related proteins in vitamin D-induced differentiation of HL-60 cells / A. Kanli, H. Savli // Experimental Oncology. — 2007. — Т. 29, № 4. — С. 314–316. — Бібліогр.: 19 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/139000 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Short communications Short communications |
| spellingShingle |
Short communications Short communications Kanli, A. Savli, H. Differential expression of 16 genes coding for cell cycle – and apoptosis-related proteins in vitamin D-induced differentiation of HL-60 cells Experimental Oncology |
| description |
Objective: The aim of the study was to analyze expression of several genes related to cell cycle regulation and apoptosis in realization of 1alpha,25-dihydroxyvitamin D3 (1,25(OH)2D3)-induced differentiation of HL-60 cells. Methods: The cultured HL-60 cells were treated with 1,25(OH)2D3. Quantitative real-time PCR was used for analyzing the changes in expression of 16 genes (Bcl-2, Bcl-xL, Mcl-1, Bik, caspase 6, caspase 7, cytochrome c, TNFR1, Myc, TGF-beta, JNK1, p38MAPK, p21, p27, Cdk2, cyclin E) at early phases of cell differentiation of HL-60 cells induced by 1,25(OH)2D3. Results: Among investigated genes, Bik and Myc gene expression was down-regulated at 48 h time points. JNK1 gene was markedly up-regulated and caspase-6 and cyclin E genes were down-regulated at 18 h time point. Conclusion: These findings suggest that there are no distinct apoptotic signals at early phases of cell differentiation. It is speculated that changes in the expression of the genes involved in vitamin D-induced apoptosis of HL-60 cells could be better visualized after the terminal stages of cell differentiation. |
| format |
Article |
| author |
Kanli, A. Savli, H. |
| author_facet |
Kanli, A. Savli, H. |
| author_sort |
Kanli, A. |
| title |
Differential expression of 16 genes coding for cell cycle – and apoptosis-related proteins in vitamin D-induced differentiation of HL-60 cells |
| title_short |
Differential expression of 16 genes coding for cell cycle – and apoptosis-related proteins in vitamin D-induced differentiation of HL-60 cells |
| title_full |
Differential expression of 16 genes coding for cell cycle – and apoptosis-related proteins in vitamin D-induced differentiation of HL-60 cells |
| title_fullStr |
Differential expression of 16 genes coding for cell cycle – and apoptosis-related proteins in vitamin D-induced differentiation of HL-60 cells |
| title_full_unstemmed |
Differential expression of 16 genes coding for cell cycle – and apoptosis-related proteins in vitamin D-induced differentiation of HL-60 cells |
| title_sort |
differential expression of 16 genes coding for cell cycle – and apoptosis-related proteins in vitamin d-induced differentiation of hl-60 cells |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2007 |
| topic_facet |
Short communications |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/139000 |
| citation_txt |
Differential expression of 16 genes coding for cell cycle – and apoptosis-related proteins in vitamin D-induced differentiation of HL-60 cells / A. Kanli, H. Savli // Experimental Oncology. — 2007. — Т. 29, № 4. — С. 314–316. — Бібліогр.: 19 назв. — англ. |
| series |
Experimental Oncology |
| work_keys_str_mv |
AT kanlia differentialexpressionof16genescodingforcellcycleandapoptosisrelatedproteinsinvitamindinduceddifferentiationofhl60cells AT savlih differentialexpressionof16genescodingforcellcycleandapoptosisrelatedproteinsinvitamindinduceddifferentiationofhl60cells |
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2023-10-18T21:19:33Z |
| last_indexed |
2023-10-18T21:19:33Z |
| _version_ |
1796152512737181696 |