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Efficacy of different immunotherapy approaches toward treatment of doxorubicin-resistant and doxorubicinsensitive transplantable rhabdomyosarcoma

Efficacy of different immunotherapy approaches toward treatment of doxorubicin-resistant and doxorubicinsensitive transplantable rhabdomyosarcoma

Aim: To evaluate the efficacy of different variants of immunotherapy, namely, adoptive LAK-therapy, vaccine therapy and their combination in vivo using transplantable murine MC-rhabdomyosarcoma resistant and sensitive to doxorubicin (Dox). Materials and Methods: The study was carried out on BALB/c m...

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Main Authors: Belova, O.B., Vinnichuk, U.D., Shlakhovenko, V.A., Berezhnaya, N.M.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2007
Series:Experimental Oncology
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Original contributions
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/139015
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spelling irk-123456789-1390152018-06-20T03:07:46Z Efficacy of different immunotherapy approaches toward treatment of doxorubicin-resistant and doxorubicinsensitive transplantable rhabdomyosarcoma Belova, O.B. Vinnichuk, U.D. Shlakhovenko, V.A. Berezhnaya, N.M. Original contributions Aim: To evaluate the efficacy of different variants of immunotherapy, namely, adoptive LAK-therapy, vaccine therapy and their combination in vivo using transplantable murine MC-rhabdomyosarcoma resistant and sensitive to doxorubicin (Dox). Materials and Methods: The study was carried out on BALB/c mice bearing Dox-sensitive and Dox-resistant transplantable murine MC-rhabdomyosarcoma. LAK-therapy (using lymphocytes from lymph nodes of syngenic mice) was performed starting from day 7 after tumor cell transplantation for 5 days; LAK (3 x 106 cells in 0.2 ml medium) were injected in the region of tumor. The vaccine prepared on the base of tumor cell glycopeptides was administered intraperitoneally at the volume of 0.2 ml before or after tumor transplantation. Efficacy of immunotherapy was evaluated by tumor growth inhibition and life span of animals. Results: By the indexes of tumor growth inhibition and average life span, for animals bearing Dox-sensitive tumors vaccine therapy was the most effective, whilst adoptive LAK-therapy was the most effective for mice bearing Dox-resistant tumors. All applied variants of therapy — adoptive LAK-therapy, vaccine therapy and their combination were effective for treatment of mice bearing Dox-sensitive and Dox-resistant transplantable murine MC-rhabdomyosarcoma. Conclusion: The obtained data demonstrated that Dox-sensitive and Dox-resistant tumors differ by the sensitivity to different types of immunotherapy. Цель: изучить влияние различных видов иммунотерапии, а именно адоптивной ЛАК-терапии, вакцинотерапии, а также их комбинации, дать оценку их эффективности в условиях перевивной МХ-рабдомиосаркомы мышей, резистентной и чувствительной к доксорубицину. Материалы и методы: исследования проведены на мышах линии BALB/c с чувствительной и резистентной к доксорубицину перевивной МХ-рабдомиосаркомой. ЛАК-терапию (лимфоцитами лимфатических узлов сингенных мышей) проводили начиная с 7 сут после перевивки опухолевых клеток на протяжении 5 дней; ЛАК вводили в область опухоли в количестве 3 млн в 0,2 мл среды. Вакцину, полученную на основе гликопептидов опухолевых клеток, вводили мышам интраперитонеально в объеме 0,2 мл по двум схемам: до перевивки и после перевивки опухоли. Влияние иммунотерапии оценивали по проценту торможения роста опухоли и выживаемости животных. Результаты: данные проведенной иммунотерапии свидетельствуют, что у мышей с чувствительной к доксорубицину опухолью наиболее эффективна вакцинотерапия, а при резистентной опухоли — адоптивная ЛАК-терапия, что подтверждалось наибольшей протяженностью жизни и наиболее выраженным торможением роста опухоли у животных этой группы. Кроме того, сравнительный анализ результатов применения ЛАК-терапии, вакцинотерапии и их сочетания показал, что все ее виды эффективны у мышей как с чувствительной, так и резистентной к доксорубицину МХ-рабдомиосаркомой. Выводы: полученные данные свидетельствуют о том, что резистентные и чувствительные к доксорубицину опухоли отличаются чувствительностью к различным видам иммунотерапии, что, по всей вероятности, объясняется различными механизмами их действия. 2007 Article Efficacy of different immunotherapy approaches toward treatment of doxorubicin-resistant and doxorubicinsensitive transplantable rhabdomyosarcoma / O.B. Belova, U.D. Vinnichuk, V.A. Shlakhovenko, N.M. Berezhnaya // Experimental Oncology. — 2007. — Т. 29, № 4. — С. 272–276. — Бібліогр.: 37 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/139015 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Belova, O.B.
Vinnichuk, U.D.
Shlakhovenko, V.A.
Berezhnaya, N.M.
Efficacy of different immunotherapy approaches toward treatment of doxorubicin-resistant and doxorubicinsensitive transplantable rhabdomyosarcoma
Experimental Oncology
description Aim: To evaluate the efficacy of different variants of immunotherapy, namely, adoptive LAK-therapy, vaccine therapy and their combination in vivo using transplantable murine MC-rhabdomyosarcoma resistant and sensitive to doxorubicin (Dox). Materials and Methods: The study was carried out on BALB/c mice bearing Dox-sensitive and Dox-resistant transplantable murine MC-rhabdomyosarcoma. LAK-therapy (using lymphocytes from lymph nodes of syngenic mice) was performed starting from day 7 after tumor cell transplantation for 5 days; LAK (3 x 106 cells in 0.2 ml medium) were injected in the region of tumor. The vaccine prepared on the base of tumor cell glycopeptides was administered intraperitoneally at the volume of 0.2 ml before or after tumor transplantation. Efficacy of immunotherapy was evaluated by tumor growth inhibition and life span of animals. Results: By the indexes of tumor growth inhibition and average life span, for animals bearing Dox-sensitive tumors vaccine therapy was the most effective, whilst adoptive LAK-therapy was the most effective for mice bearing Dox-resistant tumors. All applied variants of therapy — adoptive LAK-therapy, vaccine therapy and their combination were effective for treatment of mice bearing Dox-sensitive and Dox-resistant transplantable murine MC-rhabdomyosarcoma. Conclusion: The obtained data demonstrated that Dox-sensitive and Dox-resistant tumors differ by the sensitivity to different types of immunotherapy.
format Article
author Belova, O.B.
Vinnichuk, U.D.
Shlakhovenko, V.A.
Berezhnaya, N.M.
author_facet Belova, O.B.
Vinnichuk, U.D.
Shlakhovenko, V.A.
Berezhnaya, N.M.
author_sort Belova, O.B.
title Efficacy of different immunotherapy approaches toward treatment of doxorubicin-resistant and doxorubicinsensitive transplantable rhabdomyosarcoma
title_short Efficacy of different immunotherapy approaches toward treatment of doxorubicin-resistant and doxorubicinsensitive transplantable rhabdomyosarcoma
title_full Efficacy of different immunotherapy approaches toward treatment of doxorubicin-resistant and doxorubicinsensitive transplantable rhabdomyosarcoma
title_fullStr Efficacy of different immunotherapy approaches toward treatment of doxorubicin-resistant and doxorubicinsensitive transplantable rhabdomyosarcoma
title_full_unstemmed Efficacy of different immunotherapy approaches toward treatment of doxorubicin-resistant and doxorubicinsensitive transplantable rhabdomyosarcoma
title_sort efficacy of different immunotherapy approaches toward treatment of doxorubicin-resistant and doxorubicinsensitive transplantable rhabdomyosarcoma
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2007
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/139015
citation_txt Efficacy of different immunotherapy approaches toward treatment of doxorubicin-resistant and doxorubicinsensitive transplantable rhabdomyosarcoma / O.B. Belova, U.D. Vinnichuk, V.A. Shlakhovenko, N.M. Berezhnaya // Experimental Oncology. — 2007. — Т. 29, № 4. — С. 272–276. — Бібліогр.: 37 назв. — англ.
series Experimental Oncology
work_keys_str_mv AT belovaob efficacyofdifferentimmunotherapyapproachestowardtreatmentofdoxorubicinresistantanddoxorubicinsensitivetransplantablerhabdomyosarcoma
AT vinnichukud efficacyofdifferentimmunotherapyapproachestowardtreatmentofdoxorubicinresistantanddoxorubicinsensitivetransplantablerhabdomyosarcoma
AT shlakhovenkova efficacyofdifferentimmunotherapyapproachestowardtreatmentofdoxorubicinresistantanddoxorubicinsensitivetransplantablerhabdomyosarcoma
AT berezhnayanm efficacyofdifferentimmunotherapyapproachestowardtreatmentofdoxorubicinresistantanddoxorubicinsensitivetransplantablerhabdomyosarcoma
first_indexed 2023-10-18T21:19:22Z
last_indexed 2023-10-18T21:19:22Z
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