In vitro modification of cisplatin cytotoxicity with magnetic fluid
Aim: To study cytotoxicity of cisplatin conjugated with magnetic fluid (nanocomposite) upon exposure to magnetic field on sensitive and resistant to cisplatin MCF-7 human breast cancer cells. Methods: Cytotoxic activity was evaluated by MTT-test, intracellular iron accumulation was analyzed cytochem...
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| Дата: | 2013 |
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| Автори: | , , , , , |
| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2013
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| Назва видання: | Experimental Oncology |
| Теми: | |
| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/139099 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | In vitro modification of cisplatin cytotoxicity with magnetic fluid / V.F. Chekhun, O.V. Yurchenko, L.A. Naleskina, D.V. Demash, N.Yu. Lukianova, Yu.V. Lozovska // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 15-19. — Бібліогр.: 19 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| id |
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irk-123456789-1390992018-06-20T03:12:18Z In vitro modification of cisplatin cytotoxicity with magnetic fluid Chekhun, V.F. Yurchenko, O.V. Naleskina, L.A. Demash, D.V. Lukianova, N.Yu. Lozovska, Yu.V. Original contributions Aim: To study cytotoxicity of cisplatin conjugated with magnetic fluid (nanocomposite) upon exposure to magnetic field on sensitive and resistant to cisplatin MCF-7 human breast cancer cells. Methods: Cytotoxic activity was evaluated by MTT-test, intracellular iron accumulation was analyzed cytochemically, genotoxicity was studied by micronucleus test and DNA comet assay, ultrastructure was studied by electron microscopy techniques. Results: Nanocomposite of cisplatin was more toxic to MCF-7/S and MCF-7/CP cells compared to cisplatin in conventional pharmaceutical form. In nanocomposite-treated cells we observed more expressed signs of dystrophy (especially following application of magnetic field) and drastic alterations of nuclei ultrastructure with significant accumulation of iron nanoparticle clusters. The potent toxic action of nanocomposite is confirmed by electron microscopy and by marked genotoxicity, especially against MCF-7/CP cells. Conclusion: The enhancement of cyto- and genotoxicity of cisplatin nanocomposite combined with magnetic field in comparison with effect of convetntional cisplatin alone was demonstrated. 2013 Article In vitro modification of cisplatin cytotoxicity with magnetic fluid / V.F. Chekhun, O.V. Yurchenko, L.A. Naleskina, D.V. Demash, N.Yu. Lukianova, Yu.V. Lozovska // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 15-19. — Бібліогр.: 19 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/139099 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Chekhun, V.F. Yurchenko, O.V. Naleskina, L.A. Demash, D.V. Lukianova, N.Yu. Lozovska, Yu.V. In vitro modification of cisplatin cytotoxicity with magnetic fluid Experimental Oncology |
| description |
Aim: To study cytotoxicity of cisplatin conjugated with magnetic fluid (nanocomposite) upon exposure to magnetic field on sensitive and resistant to cisplatin MCF-7 human breast cancer cells. Methods: Cytotoxic activity was evaluated by MTT-test, intracellular iron accumulation was analyzed cytochemically, genotoxicity was studied by micronucleus test and DNA comet assay, ultrastructure was studied by electron microscopy techniques. Results: Nanocomposite of cisplatin was more toxic to MCF-7/S and MCF-7/CP cells compared to cisplatin in conventional pharmaceutical form. In nanocomposite-treated cells we observed more expressed signs of dystrophy (especially following application of magnetic field) and drastic alterations of nuclei ultrastructure with significant accumulation of iron nanoparticle clusters. The potent toxic action of nanocomposite is confirmed by electron microscopy and by marked genotoxicity, especially against MCF-7/CP cells. Conclusion: The enhancement of cyto- and genotoxicity of cisplatin nanocomposite combined with magnetic field in comparison with effect of convetntional cisplatin alone was demonstrated. |
| format |
Article |
| author |
Chekhun, V.F. Yurchenko, O.V. Naleskina, L.A. Demash, D.V. Lukianova, N.Yu. Lozovska, Yu.V. |
| author_facet |
Chekhun, V.F. Yurchenko, O.V. Naleskina, L.A. Demash, D.V. Lukianova, N.Yu. Lozovska, Yu.V. |
| author_sort |
Chekhun, V.F. |
| title |
In vitro modification of cisplatin cytotoxicity with magnetic fluid |
| title_short |
In vitro modification of cisplatin cytotoxicity with magnetic fluid |
| title_full |
In vitro modification of cisplatin cytotoxicity with magnetic fluid |
| title_fullStr |
In vitro modification of cisplatin cytotoxicity with magnetic fluid |
| title_full_unstemmed |
In vitro modification of cisplatin cytotoxicity with magnetic fluid |
| title_sort |
in vitro modification of cisplatin cytotoxicity with magnetic fluid |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2013 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/139099 |
| citation_txt |
In vitro modification of cisplatin cytotoxicity with magnetic fluid / V.F. Chekhun, O.V. Yurchenko, L.A. Naleskina, D.V. Demash, N.Yu. Lukianova, Yu.V. Lozovska // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 15-19. — Бібліогр.: 19 назв. — англ. |
| series |
Experimental Oncology |
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| first_indexed |
2023-10-18T21:19:37Z |
| last_indexed |
2023-10-18T21:19:37Z |
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