Nitric oxide coordinates development of genomic instability in realization of combined effect with ionizing radiation
The aim of this study was to investigate the ability of environmental nitrogen oxides or natural nitric oxide (NO) donors to modify free radicals balance and development of genomic instability alone or in combination with ionizing radiation. Methods: Genotoxicity and cytogenetic abnormalities were...
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| Дата: | 2013 |
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| Автори: | , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2013
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/139134 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Nitric oxide coordinates development of genomic instability in realization of combined effect with ionizing radiation / V.M. Mikhailenko, E.A. Diomina, I.I. Muzalov, B.I. Gerashchenko // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 58-64. — Бібліогр.: 42 назв. — англ. |
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irk-123456789-1391342018-06-20T03:04:45Z Nitric oxide coordinates development of genomic instability in realization of combined effect with ionizing radiation Mikhailenko, V.M. Diomina, E.A. Muzalov, I.I. Gerashchenko, B.I. Original contributions The aim of this study was to investigate the ability of environmental nitrogen oxides or natural nitric oxide (NO) donors to modify free radicals balance and development of genomic instability alone or in combination with ionizing radiation. Methods: Genotoxicity and cytogenetic abnormalities were assessed in vitro in peripheral blood lymphocytes (PBL) isolated from healthy humans or in vivo in rats PBL. Human PBL were treated with physiologically relevant NO donor — S-Nitrosoglutathione and X-ray irradiation. The inhalation treatment of animals with NO was carried out in chamber with purified gaseous NO mixed inside with air. Levels of S-Nitrosohemoglobin and methemoglobin in the blood were assessed with electron paramagnetic resonance. The total level of reactive oxygen and nitrogen species in PBL was determined fluorometrically, and serum levels of reactive oxygen species was determined by spectrophotometric assay. DNA damages were assessed by alkaline single-cell gel electrophoresis. The frequency of chromosomal aberrations in human PBL measured with the conventional cytogenetic assay in metaphase cells on short-term (52 h) and long-term (72 h) cultures. Results: Environmental nitrogen oxides or release of NO from stable complexes with biomolecules (such as S-Nitrosothiols) intensified generation of free radicals, DNA damage and development of genomic instability alone or in combination with ionizing radiation. Treatment of PBL by S-Nitrosoglutathione caused prevalent induction of chromatid type but irradiation — chromosome aberrations. The dose dependence of chromatid-type aberrations observed in human PBL after combined influence of S-Nitrosoglutathione and ionizing radiation indicates a crucial role of NO in the formation of chromosomal instability. Conclusion: NO can deregulate free radicals balance resulted in genotoxic effect, posttranslational modification of repair enzymes and thus coordinated development of genomic instability and increase of cancer risk. 2013 Article Nitric oxide coordinates development of genomic instability in realization of combined effect with ionizing radiation / V.M. Mikhailenko, E.A. Diomina, I.I. Muzalov, B.I. Gerashchenko // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 58-64. — Бібліогр.: 42 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/139134 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Mikhailenko, V.M. Diomina, E.A. Muzalov, I.I. Gerashchenko, B.I. Nitric oxide coordinates development of genomic instability in realization of combined effect with ionizing radiation Experimental Oncology |
| description |
The aim of this study was to investigate the ability of environmental nitrogen oxides or natural nitric oxide (NO) donors to modify free radicals balance and development of genomic instability alone or in combination with ionizing radiation. Methods: Genotoxicity and cytogenetic abnormalities were assessed in vitro in peripheral blood lymphocytes (PBL) isolated from healthy humans or in vivo in rats PBL. Human PBL were treated with physiologically relevant NO donor — S-Nitrosoglutathione and X-ray irradiation. The inhalation treatment of animals with NO was carried out in chamber with purified gaseous NO mixed inside with air. Levels of S-Nitrosohemoglobin and methemoglobin in the blood were assessed with electron paramagnetic resonance. The total level of reactive oxygen and nitrogen species in PBL was determined fluorometrically, and serum levels of reactive oxygen species was determined by spectrophotometric assay. DNA damages were assessed by alkaline single-cell gel electrophoresis. The frequency of chromosomal aberrations in human PBL measured with the conventional cytogenetic assay in metaphase cells on short-term (52 h) and long-term (72 h) cultures. Results: Environmental nitrogen oxides or release of NO from stable complexes with biomolecules (such as S-Nitrosothiols) intensified generation of free radicals, DNA damage and development of genomic instability alone or in combination with ionizing radiation. Treatment of PBL by S-Nitrosoglutathione caused prevalent induction of chromatid type but irradiation — chromosome aberrations. The dose dependence of chromatid-type aberrations observed in human PBL after combined influence of S-Nitrosoglutathione and ionizing radiation indicates a crucial role of NO in the formation of chromosomal instability. Conclusion: NO can deregulate free radicals balance resulted in genotoxic effect, posttranslational modification of repair enzymes and thus coordinated development of genomic instability and increase of cancer risk. |
| format |
Article |
| author |
Mikhailenko, V.M. Diomina, E.A. Muzalov, I.I. Gerashchenko, B.I. |
| author_facet |
Mikhailenko, V.M. Diomina, E.A. Muzalov, I.I. Gerashchenko, B.I. |
| author_sort |
Mikhailenko, V.M. |
| title |
Nitric oxide coordinates development of genomic instability in realization of combined effect with ionizing radiation |
| title_short |
Nitric oxide coordinates development of genomic instability in realization of combined effect with ionizing radiation |
| title_full |
Nitric oxide coordinates development of genomic instability in realization of combined effect with ionizing radiation |
| title_fullStr |
Nitric oxide coordinates development of genomic instability in realization of combined effect with ionizing radiation |
| title_full_unstemmed |
Nitric oxide coordinates development of genomic instability in realization of combined effect with ionizing radiation |
| title_sort |
nitric oxide coordinates development of genomic instability in realization of combined effect with ionizing radiation |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2013 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/139134 |
| citation_txt |
Nitric oxide coordinates development of genomic instability in realization of combined effect with ionizing radiation / V.M. Mikhailenko, E.A. Diomina, I.I. Muzalov, B.I. Gerashchenko // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 58-64. — Бібліогр.: 42 назв. — англ. |
| series |
Experimental Oncology |
| work_keys_str_mv |
AT mikhailenkovm nitricoxidecoordinatesdevelopmentofgenomicinstabilityinrealizationofcombinedeffectwithionizingradiation AT diominaea nitricoxidecoordinatesdevelopmentofgenomicinstabilityinrealizationofcombinedeffectwithionizingradiation AT muzalovii nitricoxidecoordinatesdevelopmentofgenomicinstabilityinrealizationofcombinedeffectwithionizingradiation AT gerashchenkobi nitricoxidecoordinatesdevelopmentofgenomicinstabilityinrealizationofcombinedeffectwithionizingradiation |
| first_indexed |
2023-10-18T21:19:38Z |
| last_indexed |
2023-10-18T21:19:38Z |
| _version_ |
1796152516347428864 |