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Methanolic extract of Teucrium Polium L potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines

Methanolic extract of Teucrium Polium L potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines

A growing body of evidence indicates that specific phytochemicals can increase an efficacy of cancer chemotherapy. Aim: To evaluate promoting effect of the methanolic extract of Teucrium Polium (Me-TP) on cytotoxic and apoptotic activity of anticancer drugs of vinblastine, vincristine and doxorubici...

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Main Author: Rajabalian, S.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2008
Series:Experimental Oncology
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Online Access:http://dspace.nbuv.gov.ua/handle/123456789/139206
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spelling irk-123456789-1392062018-06-20T03:12:51Z Methanolic extract of Teucrium Polium L potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines Rajabalian, S. Uncategorized A growing body of evidence indicates that specific phytochemicals can increase an efficacy of cancer chemotherapy. Aim: To evaluate promoting effect of the methanolic extract of Teucrium Polium (Me-TP) on cytotoxic and apoptotic activity of anticancer drugs of vinblastine, vincristine and doxorubicin in vitro. Methods: Skmel-3, Saos-2, SW480, MCF-7, KB, EJ and A431 cell lines were used. Cytotoxicity was determined by MTT assay and colony formation assay. The nature of interactions was analyzed by combination index (CI) method. Apoptotic cells were assessed by DAPI staining. Results: The vincristine/Me-TP, vinblastine/Me-TP and doxorubicin/Me-TP combinations showed a strong synergistic effect in the cell growth inhibition (0.13 < CI < 0.36). Similar results were observed by colony formation assay. Furthermore, the combinations of vincristine/Me-TP and vinblastine/Me-TP resulted in a massive apoptosis (> 80%) compared to effect of individual drugs (0–3%). At the additional experiments, Me-TP reduced marginally to significantly the cytotoxic effects of vincristine and vinblastine toward the human fibroblasts (p < 0.05 to 0.001). Conclusions: The present study suggests that Me-TP has the potential to be an effective and safe chemosensitizer agent for cancer therapy. Исследования последних лет показали, что некоторые фитопрепараты могут усиливать эффект химиотерапии. Цель: оценить in vitro влияние метанольного экстракта Teucrium Polium (Me-TP) на цитотоксичность и апоптоз, индуцируемые противоопухолевыми препаратами винбластином, винкристином и доксорубицином. Методы: в работе использованы клеточные линии Skmel-3, Saos-2, SW480, MCF-7, KB, EJ и A431. Цитотоксичность определяли с помощью MTT теста и реакции колониеобразования. Характер воздействия препаратов изучали с помощью метода комбинированного индекса (CI поптотические клетки выявляли окрашиванием DAPI. Результаты: для комбинаций винкристин/Me-TP, винбластин/Me-TP и доксорубицин/Me-TP было выявлено сильное синергичное воздействие на торможение клеточного роста (0,13 < CI < 0,36). Аналогичные результаты получены при определении способности клеток к колониеобразованию. Более того, комбинации винкристин/Me-TP и винбластин/Me-TP вызывали выраженный апоптоз клеток (> 80%) по сравнению с химиопрепаратами без Me-TP (0–3%). Показано также, что Me-TP снижал цитотоксическое воздействие (от минимальной до значительной степени) винкристина и винбластина на фибробласты человека (p < 0,05 до 0,001). Выводы: Me-TP можно рассматривать как возможный эффективный и безопасный химиосенсибилизирующий препарат для химиотерапии. 2008 Article Methanolic extract of Teucrium Polium L potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines / S. Rajabalian // Experimental Oncology. — 2008. — Т. 30, № 2. — С. 133–138. — Бібліогр.: 30 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/139206 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Uncategorized
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Uncategorized
Rajabalian, S.
Methanolic extract of Teucrium Polium L potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines
Experimental Oncology
description A growing body of evidence indicates that specific phytochemicals can increase an efficacy of cancer chemotherapy. Aim: To evaluate promoting effect of the methanolic extract of Teucrium Polium (Me-TP) on cytotoxic and apoptotic activity of anticancer drugs of vinblastine, vincristine and doxorubicin in vitro. Methods: Skmel-3, Saos-2, SW480, MCF-7, KB, EJ and A431 cell lines were used. Cytotoxicity was determined by MTT assay and colony formation assay. The nature of interactions was analyzed by combination index (CI) method. Apoptotic cells were assessed by DAPI staining. Results: The vincristine/Me-TP, vinblastine/Me-TP and doxorubicin/Me-TP combinations showed a strong synergistic effect in the cell growth inhibition (0.13 < CI < 0.36). Similar results were observed by colony formation assay. Furthermore, the combinations of vincristine/Me-TP and vinblastine/Me-TP resulted in a massive apoptosis (> 80%) compared to effect of individual drugs (0–3%). At the additional experiments, Me-TP reduced marginally to significantly the cytotoxic effects of vincristine and vinblastine toward the human fibroblasts (p < 0.05 to 0.001). Conclusions: The present study suggests that Me-TP has the potential to be an effective and safe chemosensitizer agent for cancer therapy.
format Article
author Rajabalian, S.
author_facet Rajabalian, S.
author_sort Rajabalian, S.
title Methanolic extract of Teucrium Polium L potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines
title_short Methanolic extract of Teucrium Polium L potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines
title_full Methanolic extract of Teucrium Polium L potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines
title_fullStr Methanolic extract of Teucrium Polium L potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines
title_full_unstemmed Methanolic extract of Teucrium Polium L potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines
title_sort methanolic extract of teucrium polium l potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2008
topic_facet Uncategorized
url http://dspace.nbuv.gov.ua/handle/123456789/139206
citation_txt Methanolic extract of Teucrium Polium L potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines / S. Rajabalian // Experimental Oncology. — 2008. — Т. 30, № 2. — С. 133–138. — Бібліогр.: 30 назв. — англ.
series Experimental Oncology
work_keys_str_mv AT rajabalians methanolicextractofteucriumpoliumlpotentiatesthecytotoxicandapoptoticeffectsofanticancerdrugsofvincristinevinblastineanddoxorubicinagainstapanelofcancerouscelllines
first_indexed 2023-10-18T21:19:57Z
last_indexed 2023-10-18T21:19:57Z
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