Expression of human beta-defensins-1, 2 and 4 mRNA in human lung tumor tissue: a pilot study

• Дата: 2008
• Автори: Shestakova, T., Zhuravel, E., Bolgova, L., Alekseenko, O., Soldatkina, M., Pogrebnoy, P.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2008
• Назва видання: Experimental Oncology
• Теми: Short communications
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/139222
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Expression of human beta-defensins-1, 2 and 4 mRNA in human lung tumor tissue: a pilot study / T. Shestakova, E. Zhuravel, L. Bolgova, O. Alekseenko, M. Soldatkina, P. Pogrebnoy // Experimental Oncology. — 2008. — Т. 30, № 2. — С. 153–156. — Бібліогр.: 19 назв. — англ.

To analyze the patterns of human beta-defensin-1, 2, 4 (hBDs) expression in human lung tumors. Materials and Methods: Tissue samples of surgically resected human lung tumors (squamous cell carcinoma (SCC), n = 10; adenocarcinoma (AC), n = 10) paired with conditionally normal tissue samples were analized for expression of hBD-1, 2, 4 mRNA by semiquantitive RT-PCR. Results: In a number of studied lung cancer tissue samples, overexpression of defensin mRNA was registered: hBD-1 mRNA (50% of SCC and 60% AC), hBD-2 mRNA (60% of SCC and 50% of AC) or hBD-4 (40% of SCC and 20% AC). No correlation was detected between the levels of hBD-1, hBD-2 and hBD-4 mRNA and histological type, differentiation grade of the tumor, and the stage of the disease, as well as the content of hBD-2 peptide in blood serum of lung cancer patients. Conclusion: Human beta-defensins-1 and -2 are often up-regulated in human lung tumors.