Effect of anticancer drugs on production of transforming growth factor and expression of P53 and BCL-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma

• Дата: 2008
• Автори: Stoika, R.S., Yakymovych, I.A., Kashchak, N.I., Boyko, M.M., Korynevska, A.V., Klyuchyvska, O.Yu., Shafranska, G.I., Yakymovych, M.Ya., Zhylchuk, V.Ye., Kudryavets, Yu.Y., Vorontsova, A.L.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2008
• Назва видання: Experimental Oncology
• Теми: Uncategorized
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/139225
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Effect of anticancer drugs on production of transforming growth factor and expression of P53 and BCL-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma / R.S. Stoika, I.A. Yakymovych, N.I. Kashchak, M.M. Boyko, А.V. Korynevska, О.Yu. Klyuchyvska, G.I. Shafranska, M.Ya. Yakymovych, V.Ye. Zhylchuk, Yu.Y. Kudryavets, A.L. Vorontsova // Experimental Oncology. — 2008. — Т. 30, № 1. — С. 35–41. — Бібліогр.: 20 назв. — англ.

Aim: To compare the capability of methotrexate, cisplatin, doxorubicine and vincristine to induce production of the transforming growth factor b1 (TGF- b1) in two cell lines — MCF-7 and T47D — of human breast carcinoma, as well as to study sensitivity of these cells to TGF- b1 and mentioned anticancer drugs. Materials and Methods: ELISA for detection of TGF- b content in conditioned culture media and Western-blot analysis of the proapoptotic p53 and antiapoptotic Bcl-2 proteins were applied. Results: t47d cells showing higher resistance to growth inhibiting effect of TGF- b1 were also refractory to cisplatin. There was no difference between MCF-7 and T47D cells in their sensitivity to methotrexate and doxorubicine, although T47D cells were more sensitive to vincristine. It was found that methotrexate and vincristine did not affect TGF- b1 production, while doxorubicine used at a dose of 1–100 ug/ml, significantly induced TGF- b1 production in both cell lines. p53 expression in T47D cells was higher than in MCF-7 cells where only doxorubicin induced strongly p53 expression. It should be noted, that Bcl-2 was better expressed in MCF-7 cells, while it was almost undetectable in T47D cells. Conclusion: In cells of human mammary carcinoma of MCF-7 and T47D lines doxorubicine, unlike vincristine and methotrexate, in dose depending manner induces production of TGF- b1. TGF- b1 production in carcinoma cells was associated with doxorubicine-mediated p53 expression in MCF-7 cells or high basal level of p53 in T47D cells. The cells of MCF-7 line were more sensitive to growth inhibition by exogenous TGF- b1 and to cisplatine action than T47D cells, but there was no difference between these cell lines in sensitivity to other anticancer drugs.