Effect of anticancer drugs on production of transforming growth factor and expression of P53 and BCL-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma

Effect of anticancer drugs on production of transforming growth factor and expression of P53 and BCL-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma

Aim: To compare the capability of methotrexate, cisplatin, doxorubicine and vincristine to induce production of the transforming growth factor b1 (TGF- b1) in two cell lines — MCF-7 and T47D — of human breast carcinoma, as well as to study sensitivity of these cells to TGF- b1 and mentioned anticanc...

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Дата:2008
Автори: Stoika, R.S., Yakymovych, I.A., Kashchak, N.I., Boyko, M.M., Korynevska, A.V., Klyuchyvska, O.Yu., Shafranska, G.I., Yakymovych, M.Ya., Zhylchuk, V.Ye., Kudryavets, Yu.Y., Vorontsova, A.L.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2008
Назва видання:Experimental Oncology
Теми:
Uncategorized
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/139225
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Effect of anticancer drugs on production of transforming growth factor and expression of P53 and BCL-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma / R.S. Stoika, I.A. Yakymovych, N.I. Kashchak, M.M. Boyko, А.V. Korynevska, О.Yu. Klyuchyvska, G.I. Shafranska, M.Ya. Yakymovych, V.Ye. Zhylchuk, Yu.Y. Kudryavets, A.L. Vorontsova // Experimental Oncology. — 2008. — Т. 30, № 1. — С. 35–41. — Бібліогр.: 20 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Uncategorized
Uncategorized
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Uncategorized
Stoika, R.S.
Yakymovych, I.A.
Kashchak, N.I.
Boyko, M.M.
Korynevska, A.V.
Klyuchyvska, O.Yu.
Shafranska, G.I.
Yakymovych, M.Ya.
Zhylchuk, V.Ye.
Kudryavets, Yu.Y.
Vorontsova, A.L.
Effect of anticancer drugs on production of transforming growth factor and expression of P53 and BCL-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma
Experimental Oncology
description Aim: To compare the capability of methotrexate, cisplatin, doxorubicine and vincristine to induce production of the transforming growth factor b1 (TGF- b1) in two cell lines — MCF-7 and T47D — of human breast carcinoma, as well as to study sensitivity of these cells to TGF- b1 and mentioned anticancer drugs. Materials and Methods: ELISA for detection of TGF- b content in conditioned culture media and Western-blot analysis of the proapoptotic p53 and antiapoptotic Bcl-2 proteins were applied. Results: t47d cells showing higher resistance to growth inhibiting effect of TGF- b1 were also refractory to cisplatin. There was no difference between MCF-7 and T47D cells in their sensitivity to methotrexate and doxorubicine, although T47D cells were more sensitive to vincristine. It was found that methotrexate and vincristine did not affect TGF- b1 production, while doxorubicine used at a dose of 1–100 ug/ml, significantly induced TGF- b1 production in both cell lines. p53 expression in T47D cells was higher than in MCF-7 cells where only doxorubicin induced strongly p53 expression. It should be noted, that Bcl-2 was better expressed in MCF-7 cells, while it was almost undetectable in T47D cells. Conclusion: In cells of human mammary carcinoma of MCF-7 and T47D lines doxorubicine, unlike vincristine and methotrexate, in dose depending manner induces production of TGF- b1. TGF- b1 production in carcinoma cells was associated with doxorubicine-mediated p53 expression in MCF-7 cells or high basal level of p53 in T47D cells. The cells of MCF-7 line were more sensitive to growth inhibition by exogenous TGF- b1 and to cisplatine action than T47D cells, but there was no difference between these cell lines in sensitivity to other anticancer drugs.
format Article
author Stoika, R.S.
Yakymovych, I.A.
Kashchak, N.I.
Boyko, M.M.
Korynevska, A.V.
Klyuchyvska, O.Yu.
Shafranska, G.I.
Yakymovych, M.Ya.
Zhylchuk, V.Ye.
Kudryavets, Yu.Y.
Vorontsova, A.L.
author_facet Stoika, R.S.
Yakymovych, I.A.
Kashchak, N.I.
Boyko, M.M.
Korynevska, A.V.
Klyuchyvska, O.Yu.
Shafranska, G.I.
Yakymovych, M.Ya.
Zhylchuk, V.Ye.
Kudryavets, Yu.Y.
Vorontsova, A.L.
author_sort Stoika, R.S.
title Effect of anticancer drugs on production of transforming growth factor and expression of P53 and BCL-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma
title_short Effect of anticancer drugs on production of transforming growth factor and expression of P53 and BCL-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma
title_full Effect of anticancer drugs on production of transforming growth factor and expression of P53 and BCL-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma
title_fullStr Effect of anticancer drugs on production of transforming growth factor and expression of P53 and BCL-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma
title_full_unstemmed Effect of anticancer drugs on production of transforming growth factor and expression of P53 and BCL-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma
title_sort effect of anticancer drugs on production of transforming growth factor and expression of p53 and bcl-2 proteins by mcf-7 and t47d cell lines of human breast carcinoma
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2008
topic_facet Uncategorized
url http://dspace.nbuv.gov.ua/handle/123456789/139225
citation_txt Effect of anticancer drugs on production of transforming growth factor and expression of P53 and BCL-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma / R.S. Stoika, I.A. Yakymovych, N.I. Kashchak, M.M. Boyko, А.V. Korynevska, О.Yu. Klyuchyvska, G.I. Shafranska, M.Ya. Yakymovych, V.Ye. Zhylchuk, Yu.Y. Kudryavets, A.L. Vorontsova // Experimental Oncology. — 2008. — Т. 30, № 1. — С. 35–41. — Бібліогр.: 20 назв. — англ.
series Experimental Oncology
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spelling irk-123456789-1392252018-06-20T03:11:11Z Effect of anticancer drugs on production of transforming growth factor and expression of P53 and BCL-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma Stoika, R.S. Yakymovych, I.A. Kashchak, N.I. Boyko, M.M. Korynevska, A.V. Klyuchyvska, O.Yu. Shafranska, G.I. Yakymovych, M.Ya. Zhylchuk, V.Ye. Kudryavets, Yu.Y. Vorontsova, A.L. Uncategorized Aim: To compare the capability of methotrexate, cisplatin, doxorubicine and vincristine to induce production of the transforming growth factor b1 (TGF- b1) in two cell lines — MCF-7 and T47D — of human breast carcinoma, as well as to study sensitivity of these cells to TGF- b1 and mentioned anticancer drugs. Materials and Methods: ELISA for detection of TGF- b content in conditioned culture media and Western-blot analysis of the proapoptotic p53 and antiapoptotic Bcl-2 proteins were applied. Results: t47d cells showing higher resistance to growth inhibiting effect of TGF- b1 were also refractory to cisplatin. There was no difference between MCF-7 and T47D cells in their sensitivity to methotrexate and doxorubicine, although T47D cells were more sensitive to vincristine. It was found that methotrexate and vincristine did not affect TGF- b1 production, while doxorubicine used at a dose of 1–100 ug/ml, significantly induced TGF- b1 production in both cell lines. p53 expression in T47D cells was higher than in MCF-7 cells where only doxorubicin induced strongly p53 expression. It should be noted, that Bcl-2 was better expressed in MCF-7 cells, while it was almost undetectable in T47D cells. Conclusion: In cells of human mammary carcinoma of MCF-7 and T47D lines doxorubicine, unlike vincristine and methotrexate, in dose depending manner induces production of TGF- b1. TGF- b1 production in carcinoma cells was associated with doxorubicine-mediated p53 expression in MCF-7 cells or high basal level of p53 in T47D cells. The cells of MCF-7 line were more sensitive to growth inhibition by exogenous TGF- b1 and to cisplatine action than T47D cells, but there was no difference between these cell lines in sensitivity to other anticancer drugs. Цель: провести сравнительный анализ способности метотрексата, цисплатина, доксорубицина и винкристина индуцировать продукцию трансформирующего фактора роста (TФР)-β1 клетками рака молочной железы человека (линии MCF-7 и T47D), а также исследовать чувствительность этих клеток к TФР-β1 и указанным противоопухолевым препаратам. Материалы и методы: для определения содержания TФР-β1 в кондиционированной среде использовали иммуноферментный анализ. Уровень белков p53 и Bcl-2 оценивали методом иммуноблоттинга. Результаты: клетки t47d, которые характеризовались большей резистентностью к ростингибирующему действию TФР-β1 по сравнению с клетками MCF-7, оказались менее чувствительными к цисплатину. Не выявлено различий между чувствительностью клеток MCF-7 и T47D к действию метотрексата или доксорубицина, хотя клетки T47D были более чувствительными к винкристину. Метотрексат и винкристин не влияли на продукцию TФР-β1 клетками MCF-7 и T47D, тогда как доксорубицин в дозе 1–100 мкг/мл существенно усиливал продукцию TФР-β1 . Экспрессия белка p53 была более интенсивной в клетках T47D, по сравнению с клетками линии MCF-7, и лишь в случае действия доксорубицина на клетки линии MCF-7 было выявлено значительное повышение его уровня. При этом белок Bcl-2 был выявлен только в клетках MCF-7. Выводы: в клеточных линиях карциномы молочной железы человека (MCF-7 и T47D) доксорубицин, в отличие от винкристина и метотрексата, дозозависимо индуцирует продукцию ТФР-β1 . Индукция доксорубицином ТФР-β1 в клетках карцином ассоциирована с индукцией экспрессии р53 в клетках MCF-7 или повышенным базальным уровнем этого белка в клетках T47D. Клетки линии MCF-7, более чувствительные к ростингибирующему действию экзогенного ТФР-β1 , чем клетки линии T47D, отличаются и более выраженной чувствительностью к действию цисплатина, но не отличаются по чувствительности от других цитостатиков. 2008 Article Effect of anticancer drugs on production of transforming growth factor and expression of P53 and BCL-2 proteins by MCF-7 and T47D cell lines of human breast carcinoma / R.S. Stoika, I.A. Yakymovych, N.I. Kashchak, M.M. Boyko, А.V. Korynevska, О.Yu. Klyuchyvska, G.I. Shafranska, M.Ya. Yakymovych, V.Ye. Zhylchuk, Yu.Y. Kudryavets, A.L. Vorontsova // Experimental Oncology. — 2008. — Т. 30, № 1. — С. 35–41. — Бібліогр.: 20 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/139225 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України

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